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1 - 11 of 11 citations for BODIPY FL DHPE since 2020

• A cell-ECM mechanism for connecting the ipsilateral eye to the brain

  Jianmin Su, et al., bioRxiv - Neuroscience 2021

  Quote: ... or anti-Fl (1:2000, Roche) antibodies followed by fluorescent staining with Tyramide Signal Amplification system (PerkinElmer) ...
• Planarian dorsoventral Netrins control a muscle midline signaling center and regulate blastema formation

  Erik G. Schad, Christian P. Petersen, bioRxiv - Developmental Biology 2022

  Quote: ... and 1:2000 for anti-FL-(Roche), or 1:4000 for anti-dig-alkaline phosphatase used in colorimetric in situ hybridizations (Roche ...
• A cell-ECM mechanism for connecting the ipsilateral eye to the brain

  Jianmin Su, et al., bioRxiv - Neuroscience 2021

  Quote: ... riboprobes were synthesized using digoxigenin (Dig) or fluorescein (Fl)-labeled UTP (Roche) and the MAXIscript In Vitro Transcription kit (Ambion) ...
• Interleukin-15 Receptor α Chain Knockout NOD-SCID mice have reduced numbers of NK cells, develop thymic lymphomas and fail to engraft human hematopoietic cells

  Andrea Vecchione, et al., bioRxiv - Immunology 2021

  Quote: ... FL fragments were treated for 20 minutes at 37°C with 100 μg/mL of Liberase (Roche) to obtain a cell suspension ...
• Follicular helper- and peripheral helper-like T cells drive autoimmune disease in human immune system mice

  Andrea Vecchione, et al., bioRxiv - Immunology 2024

  Quote: ... FL fragments were treated for 20 minutes at 37°C with 100 μg/mL of Liberase (Roche) to obtain a cell suspension ...
• DNMT1 inhibition by pUG-fold quadruplex RNA

  Linnea I. Jansson-Fritzberg, et al., bioRxiv - Biochemistry 2022

  Quote: ... Crosslinked pellets were thawed and gently resuspend in 1 mL cold Farnham Lab (FL) buffer (5mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, 1X Protease Inhibitor Cocktail (Roche)) ...
• DNMT1 inhibition by pUG-fold quadruplex RNA

  Linnea I. Jansson-Fritzberg, et al., bioRxiv - Biochemistry 2022

  Quote: ... Supernatant was removed and nuclear pellet was washed once with 1 ml 1X FL buffer (5mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, 1X Protease Inhibitor Cocktail (Roche)) ...
• Multiple ancestral duplications of the red-sensitive opsin gene (LWS) in teleost fishes and convergent spectral shifts to green vision in gobies

  Fabio Cortesi, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... were synthesized from cDNA templates via T3 RNA polymerase mediated strand-specific RNA transcription using DIG/FL RNA labelling mix (Roche). Hybridised ...
• Probing the DCAF12 interactions with MAGEA3 and CCT5 C-terminal degrons

  Germanna Lima Righetto, et al., bioRxiv - Biochemistry 2023

  Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
• Dual midbrain and forebrain origins of thalamic inhibitory interneurons

  Polona Jager, et al., bioRxiv - Neuroscience 2020

  Quote: ... the sections were incubated for 2 hours at room temperature with an anti-FL antibody conjugated with HRP (1/500, Roche Diagnostics) followed by reaction with TSA biotin reagents (Perkin Elmer ...
• GPR158 in pyramidal neurons mediates social novelty behavior via modulating synaptic transmission in mice

  Shoupeng Wei, et al., bioRxiv - Neuroscience 2023

  Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...