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Citations for Roche :
401 - 450 of 2345 citations for 4 Chloro 2 fluoro 3' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: 12-16 hours old embryos were collected and lysed in homogenization buffer (25 mM Hepes pH 7.4; 150mM NaCl; 0.5mM EDTA pH 8.0; 1 mM DTT and 1 tablet of proteinase inhibitor cocktail; Roche 11836170001). The aqueous phase of the lysate was collected after 1 hour of centrifugation at 4°C and centrifuged again for 25 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... were homogenised with 1:4 weigth per volume diethylamine (DEA) buffer (50 mM NaCl, 0.2% diethylamine, pH 10, cOmplete Protease InhibitorsTM, Roche). After 60 min at 130000xg and 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitants were washed with acetone and dissolved in SDS-PAGE sample buffer containing 4 mM Pefabloc SC (11429876001, Roche). Equivalent protein amounts were subjected to immunoblotting analysis.
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was incubated at 4°C overnight with 20 μl anti-HA antibody coated beads (Roche, Rotkreuz, Switzerland) at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Cultured cells/tissues were lysed with 4% SDS in TEA buffer (50 mM Triethanolamine, 150 mM NaCl, pH=7.3) supplemented with protease inhibitor cocktail (Roche). Total 500μg protein was treated with 10mM TCEP for 30min and then incubated with 25mM NEM for 2 h at room temperature to block unmodified cysteine residues ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated skin was incubated overnight at 4 °C in a tube containing 10 mL of 1x Dispase (Roche) and 2x Pen/Strep (Sigma ...
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bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Plant Biology 2024Quote: ... the lysate was clarified by centrifugation at 12,000 x g and 4°C for 15 min and then filtered using a 0.45-µm syringe filter (Roche). The filtered lysate was passed over a gravity-flow column packed with 0.5 ml amylose resin (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: The procedure involves decapitation of adult flies (sieving, ∼6,000 heads), pulverization, homogenization (320 mM sucrose, 4 mM HEPES, protease inhibitors (complete, 11873580001, Roche)) and differential centrifugation (from low speed to higher speed ...
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bioRxiv - Immunology 2024Quote: ... beads and lysis buffer (20 mM Tris pH 7.4, 120 mM NaCl, 1 mM EDTA, 1% Triton-X-100, 0.5% sodium deoxycholate, 1× protease inhibitor cocktail [Roche])) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonadal tissues were washed three times in 0.2× SSC at 50 °C for 15 min each and transferred to 4× SSC containing 1% blocking reagent (Roche) for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: Skin samples collected from the ventral neck area of 4-5 mice were minced in Hank’s balanced solution (HBSS) and incubated at 4°C overnight with 1.5mg/ml dispase II (Roche; 25766800). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... for 8 min followed by post-coloration using Bluing reagent for 4 min at RT (Roche Diagnostic, Meylan, France). The slides were then dehydrated (ethanol and xylene ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then rinsed with PBS and lysed at 4°C in Buffer A (1% Triton X-100, 0.5% NaDOC and 1X cOmplete Protease Inhibitor Cocktail (Roche) in PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Washed three times for 30 minutes each in TNTT at 4 ℃ to remove methanol.Blocked with 1% blocking reagents (Roche) in TNTT buffer for 2 hours at 4 ℃ to prevent no specific binding ...
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bioRxiv - Microbiology 2024Quote: ... after which the cells were harvested by centrifugation and resuspended in in lysis buffer (PBS, 4% SDS, with Roche EDTA-Free Protease Inhibitor added as per manufacturer’s recommendation) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in cold extraction buffer (50 mM Tris-HCl pH 7.5 containing 1 mM EDTA, 4 mM DTT, cOmplete Protease Inhibitor Cocktail (Roche), and 1 mM PSMF ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and centrifuged at 4000 rpm for 1 min at 4°C and run on the LightCycler 480 II (Roche) using the steps outlined in Supplemental Table 3.
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bioRxiv - Cell Biology 2024Quote: ... followed by overnight incubation at 4°C with Anti-HA- Peroxidase (High Affinity, 50 mU/ml, Roche, ref: 12013819001) and HRP-conjugated anti-actin antibody (1:5000 dilution ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche)) and incubated for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets resuspended in LB1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2 supplemented with protease inhibitors [Roche]) for 5 minutes at 4 °C followed by centrifugation (1,000g ...
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bioRxiv - Synthetic Biology 2022Quote: ... Data from 2-3 technical replicates for all three biological replicates were analyzed in LightCycler® 96 software (Roche), Google Sheets ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer2 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche), 0.5 % IGEPAL CA-630 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of MNase buffer (0.3 M Sucrose, 85 mM Tris, 3 mM MgCl2, 2 mM CaCl2, 2.5U of micrococcal nuclease: Roche 10107921001) was added in to each tube (0.5 millions of cells per tube) ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell suspensions incubated for 10 min at 4°C in SDS lysis buffer containing protease inhibitors (Complete protease inhibitor cocktail, Roche). The remaining procedures were performed as described in previous reports (Carvajal-Gonzalez et al. ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein pellet was resuspended in 100ul of Lysis Buffer (150mM NaCl, 4%SDS, 50mM TrisHCl pH 8.0, 10mM EDTA, complete protease and phosphatase inhibitor cocktails-Roche diagnostics).
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bioRxiv - Genetics 2021Quote: ... and Tvrm323 mice (n = 4) eyes at one month of age were dissected in ice- cold PBS with proteinase inhibitor (Roche) and snap frozen in eppendorf tubes on dry ice.
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bioRxiv - Microbiology 2021Quote: ... and incubated with blocking buffer (4 % skim milk in 1 × PBS) followed by incubation with the primary antibody: (monoclonal anti-GFP mice 1:5000 (Roche), rabbit anti-GFP 1:10000 ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4°C with anti-Digoxigenin antibody coupled to alkaline phosphatase (Roche, 11207733910, used at 1/4000). The last day ...
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bioRxiv - Developmental Biology 2020Quote: ... in PBST supplemented with sheep serum and BSA before being incubated overnight at 4°C with an anti-digoxygenin antibody (Roche). After removal of the antibody ...
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bioRxiv - Developmental Biology 2020Quote: To prepare nuclear extracts all procedures were done at 4°C and buffers supplemented with 1x protease inhibitors (Roche, 4693132001), 15 μM MG-132 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Neuroscience 2022Quote: ... and age-matched controls lacking evident tau pathology were gently homogenized at 4°C in 10 mL of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Genomics 2020Quote: ... and a potential large inversion on chromosome 4 (Supplementary Table 11) were labeled with digoxigenin-11-dUTP (Roche, Indianapolis, IN), biotin-16-dUTP (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... centrifuged with 306 x g for 4 min and resuspended in PBS supplemented with EDTA-free protease inhibitor (Roche, 11873580001). Cell disruption was performed using a UP50H ultrasonic processor (Hielscher ...