Labshake search
Citations for Roche :
1051 - 1100 of 7693 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
-
bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
-
bioRxiv - Biochemistry 2023Quote: ... α-Ape1 (1:5000) (Hamasaki et al., 2003), α-Prb1 (laboratory stock, 1:5000) and α-GFP (Roche, 11814460001, 1:1000), were used as primary antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
-
bioRxiv - Cancer Biology 2024Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
-
bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 mM NaF, 1 mM EDTA, 1 mM EGTA, and 1% Triton X-100, supplemented with Complete EDTA-free protease inhibitor [Roche]). Cell samples were rotated for 10 min at 4 °C to achieve lysis ...
-
bioRxiv - Developmental Biology 2023Quote: Spinal cords from wt and Sorcs2-/- mice were dissected and lysed on ice with TNE lysis buffer (Tris-base 10 mM, NaCl 150 mM, EDTA 1 mM, NP40 1%, cOmplete 1% (Roche), PhosSTOP 1% (Roche) ...
-
bioRxiv - Neuroscience 2023Quote: ... 1% (w/v) sodium deoxycholate and 1% (v/v) Triton X-100) with 1% (v/v) protease inhibitor cocktail (Roche). After homogenization ...
-
bioRxiv - Molecular Biology 2024Quote: ... the cell pellets were washed twice with 1 ml of ice-cold 1 × PBS (containing 1 × protease inhibitors (Roche 04693116001)) ...
-
bioRxiv - Genomics 2024Quote: ... The chromatin-bound beads were washed with 4 × 1 ml of wash buffer (50 mM Hepes-KOH pH 7.6, 250 mM LiCl, 1 mM EDTA, 1% NP-40, 0.7% Na-Deoxycholate, 1 × Roche cOmplete Mini) for 10 minutes on a rotating wheel ...
-
bioRxiv - Biochemistry 2024Quote: ... lysis buffer (80 mL per L of initial culture; 50 mM HEPES pH 8, 1 M NaCl, 20 mM imidazole, 1 mM TCEP, 10% glycerol + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
-
bioRxiv - Biochemistry 2024Quote: ... and the pellet was resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM DTT, 1× Benzonase, 1× protease inhibitor cocktail [Roche cOmplete]). The cells were lysed using a cell disruptor at 1.5 kbar ...
-
bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...
-
bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
-
bioRxiv - Developmental Biology 2021Quote: ... Probes were hybridized at 56°C overnight and subsequently detected by anti-DIG at 4°C overnight (1:1000; Roche 11-207-733-910) with secondary tyramide signal amplification for 2h at room temperature in the dark (AKOYA Biosciences ...
-
bioRxiv - Zoology 2024Quote: ... Samples were pre-incubated for 2 h in blocking solution (25% deactivated goat serum in PBT) and incubated overnight at 4°C in blocking solution with anti-DIG antibody conjugated to alkaline phosphatase (Roche Diagnostics, Germany; 1:2000). After several washes in PBT ...
-
bioRxiv - Cell Biology 2020Quote: ... 1% Triton-x-100 containing 1 EDTA-free protease inhibitor tablet (Roche) and 1% phosphatase inhibitor cocktails 1 and 2 (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 1 Complete Mini Protease Inhibitor Cocktail tablet (Roche) were added to 10 mL buffer ...
-
bioRxiv - Microbiology 2020Quote: ... anti-3xHA (1:2000, 12CA5, Roche or 1:4000, C29F4, Cell Signaling) anti-PfALD (1:1000 ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 % (v/v) NP40 and 1 x cOmplete Protease inhibitor cocktail (Roche)) by incubating on ice for 30 min ...
-
bioRxiv - Genomics 2021Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland) and eluted in Tris-Cl ...
-
bioRxiv - Immunology 2023Quote: ... 1% Triton X-100) containing 1×EDTA-Free Protease inhibitor cocktail (Roche) and 1 mM PMSF (Sigma-Aldrich) ...
-
bioRxiv - Genomics 2023Quote: ... 1 mM PMSF and 1×cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche) were added throughout the above procedures ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland). Genomic libraries were generated using Kapa Hyper library prep kit (Roche ...
-
bioRxiv - Genetics 2023Quote: ... 1 mM EDTA and 1 x cOmpleteTM mini protease inhibitor cocktail (Roche). PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mM EDTA and supplemented with 1 × complete protease inhibitor cocktail (Roche). DDM/CHS detergent (1%/0.1% ...
-
bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA ...
-
bioRxiv - Microbiology 2022Quote: ... 10U ml−1 DNase 1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
-
bioRxiv - Microbiology 2022Quote: ... DNase 1 10U mL−1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
-
bioRxiv - Microbiology 2022Quote: ... 10U mL−1 DNase 1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
-
bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
-
bioRxiv - Cancer Biology 2021Quote: ... tumor tissues up to 1 g were mechanically minced and digested in 2 ml RPMI1640 with 200 µg/ml Liberase TL (Roche by MilliporeSigma), 200 µg/ml Liberase DL (Roche) ...
-
bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
-
bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
-
bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The left lobe minced into small (~1-2 mm) sections and digested using 0.5 mg/mL collagenase D (Roche Diagnostics, Mannheim, Germany) and 50 U/mL DNase I (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed by addition of 250 μl lysis buffer (50 mM Tris/HCl, 500 mM NaCl, 2% w/v SDS, add freshly 1 mM DTT, 1x Roche inhibitor cocktail) followed by incubation on ice for 10 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
-
bioRxiv - Microbiology 2022Quote: ... was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sections were then treated with a blocking buffer (2 % sheep serum) for 15 min at room temperature (RT) and then incubated with anti-DIG-AP (Roche; 1:500) diluted in blocking buffer for 1 hour at RT ...
-
bioRxiv - Cell Biology 2024Quote: Human peripheral blood mononuclear cells isolated from buffy coats were activated in vitro for 1 week with 20 U/ml of IL-2 (Teceleukin; Roche, Nutley, NJ) and 5 μg/ml phytohemagglutinin (Roche ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were plated one day before transfection and were then incubated with a 2:1 ratio (μL:μg) of X-tremeGENE™ HP DNA Transfection Reagent (Roche, Ref: 6366236001) and plasmids for 20 min in Opti-MEM (GibcoTM ...
-
bioRxiv - Cell Biology 2021Quote: ... 1×proteinase inhibitors (Roche). Anti-cyclin D1 antibody (2 μg ...
-
bioRxiv - Genomics 2020Quote: ... 1× protease inhibitor (Roche), 0.4 U/μl RNase inhibitor (RNaseOUT ...