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Citations for Roche :
51 - 100 of 6789 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The sonicated sample was layered over 3 ml of S3 solution (0.88 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) in a new Falcon tube and centrifuged at 3000 × g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended pellet was layered carefully over 3 ml of S2 solution (0.35 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) and centrifuged at 1430 × g for 5 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Colorimetric signals were generated using a development solution containing 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2020Quote: ... A 2ml Dounce homogenizer was used to individually homogenize each brain in a 1:1 solution of Lysis-R:2X-RIPA buffer solution with protease inhibitors (Roche cOmplete tablets #11836153001). Each sample was sonicated three times for 7 seconds and then centrifuged at 5000g for 5min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 5mM calcium chloride and 5mM magnesium-chloride) containing an enzyme cocktail [0.5% Collagenase P (Roche, Basel, Switzerland), 0.125% Hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Magnesium chloride was added to a final concentration of 5 mM and KAPA HiFi 2x ReadyMix (Roche) was used ...
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bioRxiv - Immunology 2020Quote: ... 10 mM NaF) containing 1% protease inhibitor cocktail solution (Roche). Cell lysates were clarified by centrifugation at 13000 rpm for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Tris pH 7.2 and 1% blocking solution (Roche) at 80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by treatment with a 1% blocking solution (Roche, Germany) prepared in tris-buffered saline (TBS ...
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bioRxiv - Bioengineering 2023Quote: ... Collagenase B solution 2 mg ml−1 B (Roche, Indiana) was used for HUVEC isolation (detailed protocol in 10) ...
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bioRxiv - Biophysics 2022Quote: ... The cells were incubated with 1 x blocking solution (Roche, Blocking reagent and TBST [1 x TBS containing 0.1% Tween 20] ...
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bioRxiv - Cell Biology 2024Quote: ... Hybridizing solution (70% formamide, 1 mg/mL blocking reagent (Roche), and 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... Hybridizing Solution (70% formamide, 1 mg/mL blocking reagent [Roche, Indianapolis ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of the culture was then treated with either 5 µl of MeOH or LMB dissolved in 7:3 MeOH:H2O solution (Roche) at a final concentration of 50 ng/mL for 45 min before imaging.
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Plant Biology 2020Quote: ... and resuspended in PBS buffer without calcium and magnesium and supplemented with Protector RNase inhibitor (0.2U/ul, Roche) and ultrapure BSA (0.1% ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Microbiology 2021Quote: ... incubating in 1% blocking solution with 1:10,000 α-Digoxigenin-AP Fab fragments (Roche) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: EVs were lysed using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche) and EV concentration was determined using Pierce™ MicroBCA protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... testis albuginea was peeled and added in RIPA buffer supplemented with 1mM phenyl methyl sulfonyl fluoride (PMSF) and PIC (Roche Diagnostics, 04693132001), the solution was sonicated transiently and then on the ice for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression signals were visualized using nitroblue tetrazolium/5-bromo-4-chloro-3-indolylphosphate solutions using a standard method (Roche). Whole-mount and sectioned specimens of Ciona were observed using an SZX12 stereo microscope (Olympus ...
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bioRxiv - Developmental Biology 2022Quote: ... Signal was revealed with 4 μL of NitroBlue Tetrazolium/5-Bromo-4-Chloro-3-Indolyl Phosphate (NBT/BCIP) Stock Solution (Roche)/ml of AP reaction buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% Tween 20 in water) and incubated in NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) solution (Roche).
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bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate; Roche) until the staining appeared (overnight or up to 48 h) ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.05% Tween 20) before exchange of the pre-incubation solution for hybridization buffer containing 3 ng/µl of digoxigenin-labeled (Roche) riboprobe ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated at 37oC in a staining solution containing nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) for 20-24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated for ∼72 hours at 37°C in staining solution containing nitro blue tetrazolium and 5- bromo-4-chloro-3-indolyl-phosphate (Roche). The slides were finally washed ...
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bioRxiv - Cell Biology 2021Quote: ... washed in cold PBS, and resuspended in 2ml of buffer (200 mM Tris/HCl pH7.5, 2mM magnesium acetate) with protease inhibitors (Roche). The cells were lysed using a cell homogenizer and a 10-μm ball bearing (isobiotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in 4 times the pellet volume of sucrose buffer (10 mM HEPES, pH 7.5, 250 mM sucrose, 2 mM magnesium acetate, 1X cOmplete EDTA-free protease inhibitor cocktail [Roche]) and lysed by douncing at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 10mM potassium acetate, 0.5mM magnesium acetate, 5mM DTT and one tablet of mini EDTA free protease inhibitor tab from Roche). The cells were transferred to a 1.5ml microcentrifuge tube and centrifuged at 1000 RCF at 4°C for 1min ...
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bioRxiv - Developmental Biology 2020Quote: ... at a concentration of 1:1000 in blocking solution (0.5% Roche Western Blocking Reagent and 5% inactivated horse serum in PBSTx ...
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bioRxiv - Immunology 2023Quote: ... with HBSS solution with 1 mg/ml collagenase A (11088785103; Roche) and 40 μg/ml DNase I (10104159001 ...
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bioRxiv - Biophysics 2024Quote: ... 1 µL of 25x cOmplete protease inhibitor solution (Roche, Millipore Sigma), and water to 25 µL ...
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bioRxiv - Bioengineering 2024Quote: ... enzyme solution was made using collagenase/dispase (1 mg/ml; Roche), elastase (3 U/ml ...
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bioRxiv - Cell Biology 2022Quote: EV preparations were lysed using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche) and EV concentration was determined using Pierce™ MicroBCA protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: EV preparations were lysed using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche) and EV concentration was determined using Pierce™ MicroBCA protein assay kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
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bioRxiv - Genomics 2020Quote: ... for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2, 70% formamide, 0.5% Roche 11096176001 blocking reagent). Hybridization was carried out for 2 hours at RT in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP; Roche, Switzerland) for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: 106 cells were washed with 3 mL of cold PBS and resuspended in 50 µL solution containing 100 µg/mL neuraminidase from Clostridium perfringens (Roche, #11585886001). Cells were incubated for 1 h at 37 °C and washed twice with PBS before incubation with LiLA.
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Bioengineering 2022Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Lactate dehydrogenase (LDH) assay kit (LDH Cytotoxicity Detection KitPLUS) were obtained from Roche (Germany). MG-63 cell line (National Cell Bank of Iran (NCBI) ...
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bioRxiv - Neuroscience 2021Quote: ... medium was replaced with E6 medium (DMEM/F-12 supplemented with 64 mg/L L-ascorbic acid 2-phosphate magnesium, 14 µg/L sodium selenium, 543 mg/L sodium bicarbonate, mg/L insulin [Roche, Penzberg ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in 30 mL lysis buffer (20mM HEPES pH 7.4, 100mM potassium acetate, 2mM magnesium acetate, 3mM DTT, and EDTA-free protease inhibitor (Roche, 11836170001)) ...