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Citations for Roche :
651 - 700 of 7453 citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete protease inhibitors, Roche) and prepared for SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Microbiology 2021Quote: ... The PCR analysis was performed by mixing 5 μL of 1 μmol·L-1 of both primers and 2.5 μL of 0.25 ng·μL-1 template DNA with 12.5 μL 2× KAPA HiFi HotStart ReadyMix (Roche, Basel, Switzerland). The thermal program was 98°C for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... and the pellet was resuspended in 1-2 ml of lysis buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; protease inhibitor [Roche]), depending on the size of the cell pellet ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% NP40, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1mM Na3VO4, 4 μg/ml leupeptin, one Roche cOmplete™ protease inhibitor tablet ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete Protease Inhibitors, Roche) for 30 min on ice then spun down at 11,000 rpm for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked for 1 hour in blocking buffer consisting of 20% sheep serum and 2% blocking reagent (11096176001, Roche) in MABT ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hour and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were harvested by centrifugation and resuspended in 20 mL of lysis buffer (50 mM Tris pH 7.5, 300 mM NaCl, 2 mM MgCl2 and 1 mM DTT, 1X Roche EDTA-free Complete protease inhibitor cocktail) ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were prepared in lysis buffer (1% Triton, 1 mM DTT, 2 mM EDTA, 20 mM Tris pH 7.5 containing complete protease inhibitor, and PhosStop; Roche) from early (day 6-10 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.5% NP-40, 1 mM DTT, 1X protease inhibitor [EDTA-free complete Protease Inhibitor Cocktail, Roche, 11873580001] and 1:100 phosphatase inhibitor [Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... rat cortical neurons cultured as described above were lysed directly in buffer (50 mM HEPES pH 7.0, 2% SDS, 1 mM EDTA plus protease inhibitor mixture (PIC, Roche) and 20 mM MMTS (to block free thiols) ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... The clarified lysate was then diluted 3.75 times using dilution buffer (10 mM Tris–HCl, pH 7.4, 2 mM EDTA, 1 mM DTT, protease inhibitor cocktail tablet (cOmplete, Roche) and phosphatase inhibitor (phosSTOP ...
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bioRxiv - Immunology 2024Quote: Five female mosquitoes were collected in 250 μL of 2× RIPA buffer complemented with protease inhibitor (Complete 1×, Roche) in tubes containing ∼20 1-mm glass beads (BioSpec) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in 50% Hybridization Buffer (50% formamide, 2x SSC, 1 mg/ml Torula RNA, 0.05 mg/ml Heparin, 2% Roche blocking reagent ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in 200 μL lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, cOmplete Protease Inhibitor Cocktail, Roche), heated to 99 °C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was washed five times with 10 CV of Amylose Buffer (30 mM HEPES pH 7.6, 0.3 M NaCl, 0.1% CHAPS, 0.25 mM EDTA, 2 mM DTT, 10% glycerol, 1× Roche cOmplete protease inhibitors). Protein was eluted with Amylose Buffer supplemented with 20 mM maltose ...
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bioRxiv - Molecular Biology 2024Quote: ... SDS (0.2%), Triton X-100 (2%), sodium deoxycholate (1%), Tris HCl (100 mM, pH 8.0)) with protease inhibitor (Roche #11836145001), then incubated on ice for 30 m ...
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bioRxiv - Microbiology 2024Quote: ... Broncholalveolar lavage (BAL) fluids were obtained by intratracheal injection of 2 × 1 ml PBS supplemented with protease inhibitors (Roche). Serum was collected by intracardiac puncture and separated from whole blood in Z-Gel microtubes (Sarstedt) ...
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bioRxiv - Immunology 2024Quote: ... and incubated on ice for 1 hour and washed thrice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... and pellets were resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]). The cells were lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Microbiology 2022Quote: ... A 2-step qPCR was done using the LightCycler 480 (Roche, Anderlecht, Belgium). The activation cycle was at 95 °C for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... and 10 μl of 2× KAPA HiFi HotStart Ready Mix (KAPA Biosystems, USA). The following conditions were used ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichments were confirmed by qPCR with 2× SYBR FAST mastermix (KAPA Biosystems) using the CFX384 Real-Time System C1000 Touch Thermo Cycler (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM TCEP and an EDTA-free protease inhibitor cocktail tablet (cOmplete, Roche)) and lysed by sonication ...
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bioRxiv - Immunology 2021Quote: ... and 2 μl of 10 mg/ml of DNase (Roche, catalog no. 10104159001) in an Eppendorf tube ...
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bioRxiv - Neuroscience 2020Quote: ... Pellet was enzymatically digested in collagenase/liberase TL (2 U/mL) (Roche Diagnostics) for 1 h at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR reaction was performed on a Light Cycler 2 (Roche) using 10 μL SYBR Premix Ex Taq (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol) and supplemented with complete EDTA-free cocktail tablets (Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 mM TCEP (Tris(2-carboxyethyl)phosphine) supplemented with cOmplete protease inhibitors (Roche). Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% 2-Mercaptoethanol) with freshly added protease inhibitor and phosphatase inhibitor cocktail (Roche). Protein equivalent to 10μg was estimated by Bradford assay.
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bioRxiv - Neuroscience 2022Quote: ... followed by a 2 h incubation with Protein A Agarose beads (Roche, 11719408001) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 6 μl of 2× KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, Washington, USA), 1.4 μl of each primer (2.5 μM) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM EDTA) supplemented with EDTA-free cOmplete Protease Inhibitor Cocktail tablet (Roche) and immunoprecipitated using C9-agarose bead (Cube biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix, Roche) from the 2xStrep-tagged or 3xFLAG-tagged plasmids with oligonucleotide primers containing attB recombination sites and recombined into pDONR221 using BP clonase II (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 2 mM Dithiothreitol (DTT)) supplemented with protease inhibitor cocktail (Roche) and lysed by sonication on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellet was resuspended in 2 mL Red blood cell lysis buffer (Roche) with 5 µL DNAse (1 mg/mL) ...
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bioRxiv - Cell Biology 2022Quote: ... the 5-Bromo-2’-deoxy-uridine Labelling and Detection Kit II (Roche, UK) was used per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and supplemented with Complete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2020Quote: ... and whole transcription amplification (WTA) with KAPA HotStart HIFI 2× ReadyMix (Kapa Biosystems) for 21 cycles ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mM Na3VO4 and 0.1 U/ml Complete Protease Inhibitor Cocktail Tablets (Roche)) and incubated for 15 mins on ice ...
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bioRxiv - Plant Biology 2020Quote: ... with KAPA SYBR FAST qPCR Master Mix (2×) Kit (Kapa Biosystems, Wilmington, MA). Each primer pair was assessed for efficiency of amplification ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercapto-ethanol) supplemented with 5 μg/ml DNase I (Roche) and lysed using a homogenizer (Avestin Emulsiflex C5 ...
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bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) supplemented with a cocktail of protease inhibitors EDTA free (Roche). The lysate was clarified by centrifugation at 15000 rpm for 1h at 4°C ...