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Citations for Merck :
151 - 200 of 525 citations for 6 6' Di O tert butyldimethylsilyl D lactal since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... One tablet of 10 mg of O-Phenylenediamine dihydrochloride (Merck) was dissolved in 25 ml of citrate buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Red Oil-O catalogue Number O0625 were purchased from Merck Life Science Sp ...
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bioRxiv - Molecular Biology 2023Quote: ... formalin-fixed micromasses were stained with 0.1% Safranin-O (Merck) for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the nucleus DNA stain 4′,6-diamidino-2-phenylindole (DAPI) (1μg/ml D9542-10MG, Merck Life Science UK Ltd ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviruses were added to cells together with 6 µg/ml of polybrene (TR-1003-G, Merck Milipore, Darmstadt, Germany) and incubated for 24 hours.
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bioRxiv - Neuroscience 2024Quote: ... Entry plasmid containing tax-6 coding DNA sequence was amplified with the KOD Hot Start DNA Polymerase (Novagen; Merck). Primers were designed to contain the desired deletion and phosphorylated in 5′ end (File S4) ...
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bioRxiv - Biochemistry 2022Quote: ... 4 % glycerol) freshly supplemented with 5µg/ml Poly(dI-dC) (Merck Life Science cat. P4929) and 0.5 mM DTT ...
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bioRxiv - Microbiology 2024Quote: ... to which 10 g/L of 1,3-di-n-butyl-2 thiourea (DBT) (Merck, 8.20423.0250) was added ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained for Oil-Red-O (Merck, Sigma-Aldrich) and quantified at 520nm as described previously (Gorwood ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-O-GlcNAc clone RL2 (abcam #ab2739 and Merck Millipore #MABS157), anti-O-GlcNAc clone HGAC85 (ThermoFisher #HGAC85) ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... anti-O-GlcNAc clone RL2 (Abcam #ab2739 and Merck Millipore #MABS157). Dilution of all primary antibodies was 1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 million cells were plated per well in a 6-well plate and cultured for 48h in the presence or absence of 200 nM smoothened agonist (Merck). RNA was extracted and cDNA was generated with Superscript II kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... were assessed by analytical gel filtration using an Akta FPLC UPC-900 equipped with a Superose 6 Increase 10/300 GL column (Merck). Typically ...
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Microbiology 2021Quote: ... NHS-ester staining: the gel was placed in a 6 well plate and incubated in 594 NHS-ester (Merck, 08741) diluted at 10 μg/mL in 3 ml PBS for 1 hour and 30 minutes at room temperature on a rocking platform ...
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bioRxiv - Immunology 2020Quote: ... The thorax and the skin incision were closed with 6/0 silk sutures (Lorca Marín) and buprenorphine (0.01 mg/kg, Buprex, Merck & Co. Inc) was given for pain relief ...
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bioRxiv - Neuroscience 2022Quote: ... Following counterstaining with 4’,6-diamidino-2-phenylindole (DAPI; Vectashield/Biozol) slices were mounted in Mowiol 4-88 (Merck Chemicals).
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bioRxiv - Cancer Biology 2024Quote: ... Only treatment naive patients that underwent anti-PD-1 monotherapy with either pembrolizumab (200 mg IV every 3 weeks or 400 mg IV every 6 weeks, Merck) or nivolumab (240 mg IV every 2 weeks or 480 mg IV every 4 weeks ...
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bioRxiv - Neuroscience 2024Quote: ... the myelinated slices were incubated 16 hours at 6 DIV in 0.5 mg/ml L-α-Lysophosphatidylcholine (LPC, 830071P, Avanti, Merck) added to fresh culture medium.
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bioRxiv - Microbiology 2023Quote: ... Supernatants (35 mL) were removed at day 6 and day 10 and filtered through a Miracloth (Merck Milipore, Carrigtwohill, Ireland) into a clean tube ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Immunology 2023Quote: ... WT mice were 7-8 week-old Mice (C57BL/6 WT or Nr4a3-Timer-GS) were injected with 10 mg/kg azoxymethane (Merck) via i.p ...
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bioRxiv - Microbiology 2023Quote: R47 and R32 wild type strains were inoculated and grown in 6-well plates as described in the RNA extraction section except that 1 ml 0.38 M NaOH (Merck, Germany) was added to the middle two wells to capture HCN in the surrounding ...
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bioRxiv - Microbiology 2023Quote: ... the pH of the TSB was adjusted with hydrochloric acid solution (1M and 6 M HCL; Merck KGaA, Darmstadt, Germany) immediately prior to the experiment ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Hereafter they were added to macrophages stimulated with or without C3 (cat. no.: CAS 80295-41-6, Merck KGaA, Germany) in a macrophage:C2C12 ratio of 1:3 for 4 hours at 37 deg C ...
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bioRxiv - Neuroscience 2024Quote: ... Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck) and 500 mM sodium chloride ...
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bioRxiv - Molecular Biology 2024Quote: ... The gradient used to elute the nucleosides started by a 5-min isocratic gradient composed with 100% bufferA (10 mM ammonium acetate, pH 6) and 0% buffer B (100% methanol – 106035, Merck) with a flow rate of 0.400 ml min−1⍰and was followed by the subsequent steps ...
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bioRxiv - Microbiology 2024Quote: ... One-centimeter length of colon was homogenized (50 mg/mL) in ice-cold 50mM potassium phosphate buffer (pH 6) containing 5% hexadecyl trimethyl ammonium bromide (Merck) and hydrogen peroxide (0.0005%) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by signal development for 6–24 h in a solution containing nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Merck). The samples were covered with glass coverslips using CC/Mount (Merck) ...
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bioRxiv - Biochemistry 2024Quote: ... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
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bioRxiv - Biochemistry 2024Quote: Peak fractions from size-exclusion chromatography were concentrated to 4-6 mg mL-1 using a 50 kDa MWCO spin column (Merck), followed by addition of 30 mM D-glucose ...
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bioRxiv - Biophysics 2024Quote: ... A stripe pattern was created on the quartz surface using soft contact mode with a photomask aligned by an MA-6 mask aligner and rinsing in a developer (AZ 300 MIF, Merck) for 60 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... Protonemata were sub-cultured every 7 days and regenerated every 6 weeks from gametophores by homogenization (OMNI International) in Milli-Q H2O (Merck). Gametophore-forming colonies were cultivated and analyzed on BCD medium (BCDA without ammonium-tartrate ...
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bioRxiv - Biochemistry 2021Quote: ... O-Linked N- Acetylglucosamine Antibody (ms, clone RL2, MABS157, Merck, 1:1000), AMDHD2 (ms ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained with 1% (w/v) toluidine blue O (Merck, Germany) in 1% Na2B4O7 for 20 seconds at 50°C ...
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bioRxiv - Immunology 2021Quote: ... The plates were developed using fast o-phenylenediamine Peroxidase Substrate (Merck, #P9187), the reaction was stopped using 1M HCl ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
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bioRxiv - Plant Biology 2021Quote: ... Each exudate sample was purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and host plants was assessed by extracting the compounds following [6] by immersing the samples for 5 min in 5 ml of hexane (99%, SupraSolv, Merck, Germany), followed by removal from hexane with entomological tweezers that were previously cleaned with hexane ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...