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Citations for Merck :
1001 - 1050 of 3054 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... frozen fresh tissue (∼20-45 mg) was placed into a 2 ml sterile microcentrifuge tube pre-loaded with ∼15-20 glass beads (Merck) while 200 μL of ice-cold methanol (Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2021Quote: ... 100 mM EDTA or 200 mM Imidazole) and concentrated up to 0.5 ml using Amicon Ultra-15 centrifugal filters with MWCO of 50 kDa (Merck, Germany). The sample was then applied to a size exclusion chromatography on a Superdex 200 increase 10 /300GL column equilibrated with wash buffer ...
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bioRxiv - Biophysics 2021Quote: ... The eluted sample was exchanged into 20 mM Tris buffer and concentrated to 5 ml by Amicon Ultra-15 3 kDa (Merck). The sample was purified by 320 ml of HiLoad Superdex with a flow rate of 1 ml/min using the FPLC systems.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Major peaks were desalted by RP-HPLC using an Ascentis C18 column (3 μm, 4.6mm ID x 15 cm, Sigma-Merck, Germany) using peak-based collection (slope) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... and eluted into PBS containing 250 mM imidazole and further concentrated and desalted with an Amicon Ultra-15 Centrifugal Filter Device (Merck). The Ni-NTA affinity chromatography purified eLACCO1 protein was further applied on Superdex200pg (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was neutralised immediately using 1.5 M Tris pH 8.6 and then buffer exchanged to PBS using a 15 ml 30 kDa MWCO centrifugal filter (Merck Millipore).
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bioRxiv - Biophysics 2020Quote: ... 50 μL of sample was injected onto a Discovery BIO Wide Pore C18 column (15 cm x 4.6 mm, 5 μm column with a guard column) (Supelco, Merck, UK) and eluted on a gradient of 95% water + 0.1% acetic acid and 5% acetonitrile + 0.1% acetic acid to 5% water + 0.1% acetic acid and 95% acetonitrile + 0.1% acetic acid at a 0.8 mL/min flow-rate over 40 mins ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing MbxA were pooled and concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore). Histidine was removed with a PD10 desalting column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The elution volume of 60-70 mL was collected and the solution was concentrated to a volume of 200 μL with an Amicon Ultra-15 PLHK Ultracel-PL Membrane 100 kDa (Merck) spin column ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Biophysics 2021Quote: ... and buffer exchanged into 50 mM trisaminomethane-hydrochloride (Tris-HCl) at pH 7.4 using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). The A90C variant was labelled with 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... for 30 min at room temperature – was diluted to ∼0.002 mM DTT by three rounds of dilution/concentration in an Amicon Ultra 15 mL 3K Centrifugal Filter (Merck Millipore). TEV protease cleavage was performed overnight at room temperature.
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Biochemistry 2021Quote: ... the samples were filtered with Sterile Syringe filter (0.22 μM) and concentrated with Millipore Amicon Ultra-15 10K (Merck Millipore) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... were mixed with 120 μL of the TBA-TCA reagent (0.038% thiobarbituric acid (Kemika, Croatia) in 15% trichloroacetic acid (Merck, USA). The samples were diluted with 70 μL of ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchanges were carried out by using 15 ml Amicon Ultra Centrifugal Units with 10,000 MWCO (Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... The pellet was subjected to three freeze-thaw cycles and incubated with SAN (Merck, 50 U per 15 cm dish) for 1 h at 37 C ...
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bioRxiv - Microbiology 2023Quote: ... The volume of concentrated viral fractions (>50 kDa) was further reduced using Amicon Ultra 15 ml Centrifugal Filters (Merck-Millipore) prior to phenol/chloroform extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Immunology 2023Quote: ... Fractions with CPC-activated PO activity were pooled and concentrated to 1.1 ml using AmiconTM Ultra-15 30K centrifugal units (Merck Millipore). Proteins were separated by Sephacryl S-300 column (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Molecular Biology 2024Quote: ... The upper aqueous phase was then transferred to a new tube and mixed with 240 μL isopropanol (catalog no. 15-2320, Merck), to precipitate the RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... was filtered through a 0.45 μm syringe filter and 50-60× concentrated using Amicon Ultra-15 100 kDa NMWCO columns (#UFC910024, Merck Millipore). The resulting concentrate was aliquoted and stored at -80°C until further use.
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bioRxiv - Neuroscience 2023Quote: ... Sections were caught and rinsed in 1x PBS (3 × 15 min) prior to incubation in blocking solution (5% NDS (Merck) in 0.3% PBS-Triton-X-100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peak fractions were pooled and concentrated to 5 μM (0.7 mg/mL) final concentration using an Amicon Ultra-15 (Merck Millipore) for further analysis.
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions were pooled and concentrated using a 30 kDa cut-off Amicon Ultra-15 concentrator (Merck, cat No. UFC903024) to a typical concentration of about 6 mg/ml ...
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bioRxiv - Biophysics 2022Quote: ... Fraction 4-6 of three different columns were pooled and concentrated to 0.5 mL using an Amicon Ultra-15 10K filter (Merck Millipore). This was then placed on top of the gradient.
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bioRxiv - Microbiology 2022Quote: ... ZIKV was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-100 membrane (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Physiology 2022Quote: ... They were then permeabilized with 0.02% Triton X-100 in PBS for 15 min and blocked with 5% bovine serum albumin (Sigma-Aldrich, Merck) in PBS for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK-293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Developmental Biology 2024Quote: ... of dialyzed supernatant of organoids which was ultra-filtered into approximately 12-24- fold concentration using AMICON ULTRA-15 10KDA ULTRACEL-PL MEMBRANE (Merck), 50 Units/kg rhFVIII ...
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bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
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bioRxiv - Genetics 2024Quote: ... Cells were washed with water thrice and released into YPD media containing 15 µg mL-1 nocodazole (Cat. No. M1404, Merck) for 90 min ...
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bioRxiv - Neuroscience 2024Quote: ... or (2) collected 3 days after transfection and enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Viral pellets were resuspended in sterile PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Virus particles were purified from the cell lysate and supernatant using ultracentrifugation with OptiPrep (Serumwerk Bernburg AG) and concentrated using ultrafiltration with Amicon Ultra-15 (UFC903024; Merck).
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bioRxiv - Molecular Biology 2024Quote: ... Purification of the labelled proteins was done by repeated filtration in a 3 kDa filter (Amicon Ultra-15 Centrifugal Filter Unit, Merck).
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bioRxiv - Molecular Biology 2024Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 30,000 molecular weight cut-off (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... Peak fractions from the HisTrap column were pooled and concentrated in Amicon Ultra-15 concentrators 50,000 molecular weight cut-off (Merck Millipore). The N-terminal MBP tag was removed by TEV cleavage as described above for MPP8 ...
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bioRxiv - Biochemistry 2024Quote: ... The first step used a HiPrep 16/60 Sephacryl S-500 HR column and the QtEnc-containing fractions were concentrated using Amicon Ultra-15 filters (100 kDa MWCO, Merck) before being applied to a Superose 6 Increase 10/300 GL column ...
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bioRxiv - Molecular Biology 2024Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was concentrated until obtaining 50 ml of each cell line using Ultra-15 Centrifugal Filter Concentrator (Merck, Germany) and filtrated with 0.2 µm filter to eliminate larger vesicles ...
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bioRxiv - Bioengineering 2024Quote: ... and the flow through from Q-HP column was concentrated in Ultra-15 centrifugal filters (Ultracel-30K, Merck Millipore UFC903024) to concentration of ∼10 mg ml-1 (UV280) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI, 1 µg/mL, Merck) in OWB-SDS at 4 °C overnight on a rolling mixer (30 r.p.m.) ...
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bioRxiv - Molecular Biology 2020Quote: ... A mixture (2:1 v/v) of (PFA 4% (Merck, 104005) in PBS 1X pH7.4):OCT (Leica Surgipath ...