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Citations for Merck :
951 - 1000 of 1102 citations for 4 4' Bi 7H benz de anthracene 7 7' dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Seeds were surface-sterilized by 70% ethanol and washed 4 times with sterilized ultra-pure water (Milli-Q water, Merck-Millipore) followed by stratification at 4 ºC for 2 d ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation 100 μL of 2X STOP buffer (NaCl 340 mM, EDTA 20 mM, EGTA 4 mM, 5% Digitonin, 100 μg/mL RNase A (Merck, R6513), 50 μg/mL Glycogen (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
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bioRxiv - Bioengineering 2023Quote: ... filtered through 0.45 µm syringe filters (for Cas9/sgRNA delivery experiments) and concentrated using 100kDa MWCO Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore) to volumes of 100-250 µL.
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bioRxiv - Neuroscience 2023Quote: ... rats were anesthetized and underwent transcardial perfusion with 100 mL of saline followed by 100 mL of 4% paraformaldehyde (Merck, Germany) at 72 h following ICH induction ...
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bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... femurs and tibias of C57BL/6J mice were extracted and crushed on a mortar in PBS supplemented with 4%FBS and 2 mM EDTA and filtered through 0.45μm strainers (Merck Millipore, Cat#SLHV033RB). For B cells ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at 4°C with primary antibodies against AVP neurophysin II (NP-II; MERCK, MABN845, PS41; 1:200); OXT NP-I (PS38 ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing BirA were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Biophysics 2024Quote: ... The isolated protein was concentrated using Amicon® Ultra centrifugal filter with a molecular cutoff 10 kDa (Amicon Ultra-4 Centrifugal filter unit 10K, Merck). The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250 ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Remaining chromatin extracts were incubated overnight at 4°C with 50 µL of prewashed anti-Flag M2 magnetic beads suspension (Millipore, Merck M8823) per sample ...
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bioRxiv - Plant Biology 2024Quote: ... 6xHis:SidK:3xFLAG or 6xHis:SidKF62A:3xFLAG protein were added to 1 ml of filtered extract to a final concentration of ∼2.5 μM and incubated in a rotatory mixer at 4°C with 25 μl of Anti-FLAG® M2 Magnetic Beads (Merck). 30 μl of the mixture was taken as input for Western blot analysis before adding magnetic beads ...
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies (synaptophysin Cat#MAB329, Merck Millipore; GluA2 Cat#13607, Cell Signaling; Psd95 Cat#MAB1598, Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... Elution sample was concentrated at 3,300 rpm for 5 minutes per round until it reached 500 uL with Amicon Ultra-4 30K centrifugal filter system (Merck Millipore). Size exclusion chromatography was performed with Superdex200 increase 10/300 column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Pan-Biotech) while the other retina was incubated for 4 h in the same medium supplemented with 100 µM leupeptin (Merck, L2884) and 20 mM NH4Cl (Merck).
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bioRxiv - Immunology 2024Quote: ... ankle tissues were soaked in 4% (v/v) paraformaldehyde phosphate buffer solution (Fujifilm Wako) overnight and then replaced with OSTEOSOFT (Merck Millipore) every three days over 11 days for decalcification ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was then collected and filtered with a 3 kDa filter (Amicon Ultra-4 centrifugal Filter 3 kDa MWCO, Merck UFC8003243). Five distinct preparations of ACS <3kDa were prepared from five astrocytes cultures and were then sent to the Protein Analysis Facility of the University of Lausanne for Mass Spectrometry analysis.
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Samples for VFA determination were further fixed by adding 1% v/v of 4 M sulfuric acid (H2SO4, Merck Millipore, Germany). The concentrations of VFAs and sulfate were determined using a 930 Compact IC Flex 1 with a Metrosep A supp 7 −250/4.0 column coupled with an 887 Professional UV/VIS detector (Metrohm ...
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bioRxiv - Microbiology 2024Quote: ... The dried lipid A samples were resuspended in 30 μl of chloroform/methanol mixture (4:1) for thin-layer chromatography (TLC) analysis using Silica Gel 60 F254 TLC plate (Merck Millipore) with the solvent system consisting of chloroform/pyridine/96% formic acid/water (50/50/14.6/4.6) ...
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bioRxiv - Cell Biology 2023Quote: ... The immunoreactive bands were detected by using enhanced chemiluminescence (ECL) reagent (Immobilon Crescendo Western HRP substrate; Merck Millipore, DE). Band densities were quantified by densitometry using Image LabTM Version 6.0.1 2017 (Bio-Rad Laboratories ...
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bioRxiv - Zoology 2024Quote: ... Fish were anesthetized with MS-222 (0.1 g l-1 of sea water) (Sigma-Aldrich; Merck group, Darmstadt, DE) before each sampling and injection procedure ...
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bioRxiv - Biophysics 2022Quote: ... bis-acrylamide (2% w/w) and ammonium persulfate (0.05% w/v) (all from Merck, Germany) in 10 mM Tris-buffer (pH 7.48) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Acetylthiocholine iodide (ATCh) and 5,5’-dithio-bis-2-nitrobenzoic acid (DTNB) were obtained from Merck (https://www.sigmaaldrich.com/united-kingdom.html ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).