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Citations for Merck :
801 - 850 of 6557 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Genomics 2023Quote: ... 4 % DDT and 2 % clothianidin-impregnated papers prepared in acetone and Dow Corning 556 Silicone Fluid (BHD/Merck, Hesse, Germany). Flies overexpressing CYP6Z2 were also exposed to the primary product of hydrolysis of pyrethroids ...
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bioRxiv - Genomics 2024Quote: ... About 2 mL of supernatants were then transferred to ultrafiltration tubes (Amicon® Ultra-4 Centrifugal Filter Unit 10K, Merck). 2 mL of TE buffer was added ...
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bioRxiv - Cell Biology 2022Quote: ... the nuclei were visualized with Hoechst 33258 (Merck, B2883, 2 µg/ml in H2O at RT for 5 min).
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... whose composition is as plating media but with 5% FBS and 2 μM cytosine β-d-arabinofuranoside (Merck, C6645) to limit glial growth ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM L-glutamine and 1 mM sodium pyruvate (all Merck). The oxygen consumption rate (OCR ...
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bioRxiv - Neuroscience 2021Quote: ... 2′,7′-Dichlorofluorescin diacetate (DCFH-DA, Merck, Darmstadt, Germany, 1 mM) dissolved in PBS was added to each well and the plate was placed in the dark for 10 min at room temperature for cell uptake ...
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bioRxiv - Neuroscience 2020Quote: ... chicken anti-microtubule-associated protein 2 (MAP2; 1:2000; Chemicon/Merck), and guinea pig anti-vesicular glutamate transporter 1 (VGLUT1 ...
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bioRxiv - Neuroscience 2024Quote: ... combined with rabbit anti- MAP-2 (Merck Millipore, #AB5622, 1:800), followed by goat anti-mouse Dylight405 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2022Quote: ... and 4-aminopyridine (4-AP) (100 μM, Merck) was bath perfused to isolate direct monosynaptic inputs during photostimulation.
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Immunology 2024Quote: ... 2010) that recognizes a common epitope on MASP-1,-3 and MAP-1 followed by HRP-conjugated streptavidin (Merck, RPN1231). The plates were revealed using TMB One as the substrate (Kementec ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were concentrated 50-fold using Amicon Ultra-4 centrifugal filters with a molecular weight cut-off of 3 kDa (Merck-Millipore, Burlington, MA, USA). Samples were mixed with 3× Laemmli loading buffer and heated for 3 min at 85 °C before SDS-PAGE analysis ...
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bioRxiv - Biochemistry 2023Quote: ... was cultivated anaerobically in proline-containing CDMM (0.76 g/L consisting of 0.1 g/L added to the 0.66 g/L of the casamino acids as characterized for this casamino acids lot, Merck) and harvested by centrifugation in the late exponential phase ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... slides were washed 3×10 min in PBS and counterstained with PI (Merck, 1:500) and p-Phenylenediamine dihydrochloride (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and 188 μM L-α-phosphatidylcholine: L-α-phosphatidylinositol PC:PI (3:1) (Merck, Darmstadt, DE). 1.5 ml of 0.1 M potassium phosphate buffer ...
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bioRxiv - Plant Biology 2024Quote: ... at a dilution of 1:1000 in 3% BSA and 0.5% Triton X-100 (Merck) overnight (o/n) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured in LHC9/RPMI 1640 (1:1) without serum as previously described51,52 supplemented with rho-associated protein kinase 1 inhibitor (Y-27632, Merck, 5 mM), SMAD-signaling inhibitors ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM 2-mercaptoethanol] and then vortexed with an approximately equal volume of glass beads (212–300 mm) for 3 minutes (Merck KGaA, Germany). The homogenate was centrifuged at 12,000 g for 8 min at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... DMSO (0.1%; D4540), L-glutamic acid (L-glutamate, 1 mM; G1251) and Sodium Propionate (5mM; P1880) were obtained from Merck Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Micrococcin P1 was produced as described previously and solubilized to a concentration of 10 mg/ml in dimethyl sulfoxide (Sigma-Aldrich).30 Nisin A was obtained as part of a commercial fermentate which was solubilized to a concentration of 1 mg/ml in a 0.05% acetic acid solution (Merck). AuresinePlus was solubilized to a stock concentration 1 mg/ml in a buffer containing 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
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bioRxiv - Microbiology 2022Quote: ... 108 - 109 plaque-forming units (PFU) mL-1 in tryptone soya broth (TSB, Oxoid) or in quarter-strength (1/4) Ringer’s buffer (Merck) unless stated otherwise ...
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bioRxiv - Microbiology 2023Quote: ... 108 – 109 PFU (plaque-forming units) mL-1) in TSB (tryptone soya broth, Oxoid) or quarter-strength (1/4) Ringer’s buffer (Merck)) was used in this study ...
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bioRxiv - Microbiology 2022Quote: ... To each tube 1 μL of Tris((1-benzyl-4-triazolyl)methyl)amine (TBTA) solution (2.5 mM in DMSO; Merck), 10 μL of Tetrakis(acetonitrile)copper(I ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were permeabilised with 0.5% Triton X-100/PBS for 1 hour and blocked for 1 hour with 4% donkey serum/PBS (Merck), both at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Plant Biology 2024Quote: In some of the experiments the samples were exposed to 5 mM concentration of the phosphatase inhibitor NaF (CAS#: 7681-49-4; Merck, Darmstadt, Germany) that prevents the dephosphorylation of LHCII and therefore also the reversal of light acclimation from state 2 to state 1 (Rintamäki et al ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a mixture of the antibody constructs with a 10-fold molar excess of DFO*-NCS (5 mg/mL in DMSO) (ABX, Radeberg, Germany) was incubated ON at 4 °C with gentle shaking (Eppendorf ThermoMixer, Merck, Darmstadt, Germany). The DFO*-derivatized constructs were purified by SE-HPLC under the same conditions as described above.
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bioRxiv - Cell Biology 2024Quote: ... both untreated and treated cells at a concentration of 4-5×104/ml were placed in PBS with monochlorobimane (50 µM mCB; Merck/Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... cryoprotected for 48 h in 20% sucrose at 4°C and then snap frozen in isopentane (2-methylbutane, Merck GmbH, Austria) for 3 min at −60°C ...
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bioRxiv - Cell Biology 2022Quote: ... femurs and tibias of C57BL/6J mice were extracted and crushed on a mortar in PBS supplemented with 4%FBS and 2 mM EDTA and filtered through 0.45μm strainers (Merck Millipore, Cat#SLHV033RB). For B cells ...
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bioRxiv - Cell Biology 2024Quote: ... 0.8 μg/ml of 4-hydroxytamoxifen (4-OHT, Merck) was added to the culture medium for 48 hours to induce Cre-mediated excision of the promoter and first exon within the Oct4floxalleles ...
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bioRxiv - Plant Biology 2021Quote: ... 14-3-3 binding was detected using anti-GST monoclonal antibody (Merck).
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bioRxiv - Microbiology 2021Quote: ... were diluted 1:500 in 4% bovine serum albumin (BSA) (Sigma-Aldrich, Merck, UK).
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bioRxiv - Microbiology 2024Quote: ... and then treated with 1 mL of bleaching solution [4% Sodium hypochlorite (Merck 61842010001730) + 1N Sodium hydroxide (HiMedia MB095-100G ...
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bioRxiv - Neuroscience 2020Quote: Cell-cycle kinetic differences were assessed by labelling cortical progenitor cells using a nucleotide analog 5-ethynyl-2’-deoxyuridine (EdU; Merck) in vivo following 150 □g EdU injection into the peritoneal cavity of pregnant mice 1 hour before the sacrifice of the embryos ...
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Revisiting a GWAS peak in Arabidopsis thaliana reveals possible confounding by genetic heterogeneitybioRxiv - Genetics 2021Quote: ... 2 µl of each sample was injected into a SeQuant ZIC-pHILIC HPLC column (Merck, 100 × 2.1 mm; 5 µm), and the respective guard column operated with an Ultimate 3000 HPLC system (Dionex ...