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Citations for Biotek :
1 - 33 of 33 citations for WesternBright Quantum HRP Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... HRP activity was measured at 450 nanometer using tetramethylbenzidine substrate (BioFX) and an ELISA plate reader (EL-808, Biotek). Half-maximum effective concentration (EC50 ...
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bioRxiv - Immunology 2020Quote: ... HRP activity was measured at 450 nanometer using tetramethylbenzidine substrate (BioFX) using an ELISA plate reader (EL-808, Biotek). Half-maximum effective concentration (EC50 ...
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bioRxiv - Immunology 2021Quote: ... either 25 μL of Nano-glo substrate or CTZ substrate was added to each well and the Biotek Synergy Mx plate reader (Biotek, VT) was used to measure luminescence ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescence intensities were monitored continuously during substrate hydrolysis on Synergy 2 (BioTek®) plate reader for 120 minutes.
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bioRxiv - Biochemistry 2019Quote: ... Substrate cleavage was monitored using an all-in-one microplate reader (Gen5; BioTek) by exciting at 380 nm and following the increase in fluorescence emission at 460 nm ...
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bioRxiv - Microbiology 2023Quote: ... and substrate degradation was measured at 405 nm using Synergy HT (BioTek, Agilent). The experiment was repeated three times for reproducibility.
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bioRxiv - Cell Biology 2023Quote: ... HRP activity was measured in an HTX microplate reader (Biotek, Winooski, VT) at a wavelength of 450 nm ...
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bioRxiv - Immunology 2022Quote: ... substrate was added and the absorbance of 405 nm was measured by a microplate reader (BioTek). ED50 (serum dilution ...
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bioRxiv - Immunology 2022Quote: ... HRP-mediated ECL luminescence was read using a Synergy Neo2 HTS plate reader (BioTek) to detect IgG ...
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bioRxiv - Cell Biology 2023Quote: ... Heme-reconstituted HRP activity was measured at 652 nm using a Synergy H1 plate reader (BioTek) and heme content was interpolated using a hemin-ApoHRP standard curve.
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bioRxiv - Cell Biology 2020Quote: ... the luminescence signal was detected using a HRP detection reagent and a microplate reader (Synergy H4, Biotek).
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bioRxiv - Microbiology 2021Quote: ... 75,000 effector cells/well were added to the plate and incubated for 6 h at 37°C.The luciferase substrate was added in the dark and the luminescence activity measured after 5 minutes using a Synergy Hybrid Reader (BioTek). The data was analysed using GraphPad prism 7.
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bioRxiv - Bioengineering 2022Quote: ... The substrate was added at 50 µL/well and chemiluminescence was measured in a microplate luminescence reader (BioTek, Synergy 2).
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bioRxiv - Bioengineering 2021Quote: ... The substrate was added at 50 μL/well and chemiluminescence was measured in a microplate luminescence reader (BioTek, Synergy 2).
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bioRxiv - Immunology 2019Quote: ... and then TMB substrate (Thermo) was added to observe changes in absorbance at 450 nm with a microplate spectrophotometer (BioTek).
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bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... The substrate was added at 50 µL/well and chemiluminescence was measured in a microplate luminescence reader (BioTek, Synergy 2).
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bioRxiv - Biochemistry 2020Quote: ... was used as a substrate for the peroxidase and intensity of the reaction was proportional to absorbance measured at 450 nm with SynergyMx (BioTek) after addition of 50 μl H2SO4 at 1M.
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bioRxiv - Bioengineering 2022Quote: ... Pellet was then resuspended in 1 mL of M9 medium and flasks were inoculated to the starting OD600 of 0.1 and incubated at 30 °C with agitation (233 RPM for cultures with substrate concentration 4 g/L and 275 RPM for cultures with substrate concentration 8 g/L) using NB205 orbital incubator (N-Biotek). The ratio of medium to flask volume was 1:5 (20 mL:100 mL ...
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bioRxiv - Microbiology 2022Quote: ... 355/460 nm for ACC substrates) was measured every minute for 30 min at 37 °C on a Cytation 3 plate reader (BioTek). Initial velocities were quantified by calculating the slope of the linear phase of the reaction as relative fluorescent units (RFUs ...
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bioRxiv - Microbiology 2023Quote: ... The activity of Renilla luciferase was read immediately after adding substrate h-Coelenterazine (Nanolight Technology) on a Synergy H1 Hybrid Multi-Mode Reader (BioTek). Experiments were performed in triplicate at least ...
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bioRxiv - Molecular Biology 2021Quote: ... 96-well plate).The cells were lysed by TNT lysis and added to luciferase substrate solution and luminescence was measured using a Synergy HT (BioTek, USA).For intracellular CFU enumeration ...
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bioRxiv - Neuroscience 2022Quote: ... chromogen substrate solutions were added and plate reading conducted in a microplate photometer (SyneryTM Neo2 Multi-mode Microplate Reader; BioTek Instruments) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... immediately after adding fluorogenic substrate with irradiation at 325 nm on a Biotek Synergy H1 plate reader (BioTek, Winooski, VT, USA). Normalized velocities were fitted in MATLAB Curve Fitting Toolbox (MathWorks ...
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Discovery and Optimization of Inhibitors for the Pup Proteasome System in Mycobacterium tuberculosisbioRxiv - Microbiology 2019Quote: ... followed by 5 μL substrate in buffer (5 μL, 750 nM) after 30 min incubation were added via a MultiflowFX dispenser (Biotek, Winooski, VT). Single point fluorescence of the reactions was measured after 60 minutes using a CLARIOstar plate reader (BMG Labtech ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody binding was quantified using the Pierce TMB Substrate Kit (Thermo, Cat. 34021) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody binding data were analyzed by nonlinear regression (One site specific binding with Hill slope ...
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bioRxiv - Immunology 2020Quote: ... Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL, cat. #50-76-00) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody data were analyzed by nonlinear regression (One site total binding ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL, cat. #50-76-00) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody data were analyzed by nonlinear regression (One site total binding ...
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bioRxiv - Molecular Biology 2023Quote: ... 200nM substrates (unmodified or preubiquitylated) was mixed in and the extract was incubated at 30C on a plate reader (Biotek Synergy H1). Signal was detected every minute by a build-in monochromator (ex ...
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bioRxiv - Immunology 2021Quote: ... The immune complex was detected by adding tetramethyl-benzidine substrate solution and the absorbance was determined at 450 nm on a microplate reader (BioTek Synergy H1 Hybrid).
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bioRxiv - Biochemistry 2020Quote: ... using the FRET-based substrate Abz-SAVLQSGFRK(DNP)-NH2 wherein peptidolysis was measured at 320/420 nm (ex/em) (Biotek® Synergy M2) in the presence and absence of 0-50 μM K777 in duplicate ...
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bioRxiv - Systems Biology 2022Quote: ... The fluorogenic substrate provided with the kit was added and the plate was read for 30 minutes using a fluorometer (Biotek Synergy H1 microplate reader) at Ex:380/EM:460 nm.