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Citations for Biotek :
1 - 47 of 47 citations for Valproic Acid Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acid concentration was measured using a microplate reader Synergy HT (Biotek, Germany), with the software Gen5™ (Biotek ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... with the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... and yields were quantified the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software ...
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bioRxiv - Plant Biology 2020Quote: ... The total amino acid content was then measured in a Synergy HT plate reader (BioTek, Germany) at a wavelength of 550 nm ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 mM nitrilotriacetic acid) and FP determined with a Synergy 2 plate reader (BioTek, Winooski, VT) as described (Patriarchi et al. ...
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bioRxiv - Microbiology 2020Quote: ... and RNA concentration was measured by nucleic acid quantification in Epoch Microplate Spectrophotometer (BioTek, Winooski, VT, USA). Transfer RNAs and 5S RNA were then removed using the MEGAclear Kit Purification for Large Scale Transcription Reactions (AMBION ...
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bioRxiv - Neuroscience 2024Quote: ... The extinction of the bicinchoninic acid (BCA) was measured at 562 nm utilizing the µQuant spectrometer (BioTek). A standard curve was set up using albumin to determine the protein concentration of each sample.
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bioRxiv - Cancer Biology 2021Quote: ... Dimerization of Homovanilic acid was recorded every 30 seconds for 20 minutes with a BioTek Synergy H1 platereader (BioTek) spectrophotometer with an Ex = 320 nm and Em = 420 nm.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). A standard curve was generated using rec 2-12C antibody (Absolute Antibody) ...
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bioRxiv - Immunology 2021Quote: ... Reactions were quenched with 1 N hydrochloric acid and absorbance was measured at 450 nm using a spectrophotometer (Biotek).
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bioRxiv - Microbiology 2023Quote: ... and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay, ThermoScientific) on an Eon spectrophotometer (BioTek). Total protein lysates were separated via SDS-PAGE and normalized across the isolates using Stain-FreeTM (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were destained in destaining solution (40% EtOH and 10% acetic acid) and crystal violet quantitated at OD595 (Biotek). To measure cell clonogenicity ...
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bioRxiv - Immunology 2021Quote: ... TMB substrate was added and incubated for 2-5 min and reaction was stopped with 50µl of 1M sulphuric acid and absorbance was measured at 450 and 630nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titre was defined as the final dilution of serum that caused ≥50% reduction in NP expression.
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bioRxiv - Microbiology 2019Quote: ... The reaction was stopped with 2M sulfuric acid and absorbance read at 450 nm with a Synergy H1 micro plate reader (BioTek).
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bioRxiv - Immunology 2020Quote: ... substrate the reaction was stopped with 1M sulfuric acid and absorbance was measured at 450 nm and 570 nm (reference wavelength) on the Cytation3 Imaging Reader (Biotek). The MN titers were expressed as half maximal inhibitory dilution (50% Inhibitory titer is ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 50µL of 3M hydrochloric acid and then absorbance (at 490nm) was immediately determined using Synergy H1 hybrid multimode microplate reader (BioTek). PRISM 7.0 was used to determine the effective concentration of each virus that would yield detectable NA activity ...
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bioRxiv - Microbiology 2022Quote: ... and sulfuric acid and quantified at 450 nm using a BioTek Synergy H1 plate reader (BioTek Instruments Inc., Winoski, VT). Values were analyzed in Prism 9.0 (GraphPad ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed twice with PBS and stained biofilms were resuspended in 7% acetic acid (v/v) and absorbance was read at 595 nm in a Synergy H1 hybrid multimode reader (BioTek).
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bioRxiv - Molecular Biology 2022Quote: ... stopping the reaction with 2M sulfuric acid and by measuring the absorbance at 450nm with a microplate spectrophotometer (Epoch BioTek).
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Microbiology 2021Quote: ... stopped using 3M hydrochloric acid (HCL) solution and plates were read at 490nm wavelength using ELISA plate reader (BioTek Instruments). The endpoint antibody titer was determined by using a cut-off value which is three-times the absorbance of first dilution of mock (uninfected ...
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Microbiology 2022Quote: ... The absorbance was quantified at 570 nm using 30% acetic acid in water as the blank by a plate reader (Synergy H1 from BioTek).
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bioRxiv - Cancer Biology 2023Quote: ... Colonies were solubilized in 10% acetic acid to quantify clonogenic capacity and read for absorbance at 590nm in a plate reader (BioTek).
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bioRxiv - Biochemistry 2022Quote: ... Reactions were quenched with 2 M sulfuric acid before determining absorbance at 450 nm using a Synergy BioHTX plate reader (BioTek). The background value from wells containing no serum was averaged and subtracted from all wells on the plate.
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bioRxiv - Immunology 2024Quote: ... Reaction was stopped using 0.5 M/1N sulfuric acid and absorbance measured at 450 nm with subtracting reference at 620 nm using ELISA plate reader (Epoc, BioTek). For K18-hACE2 mice challenged with D614G for unspecific binding ...
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bioRxiv - Microbiology 2021Quote: ... 170 μL of glacial acetic acid were added to each well and the plate was read in a Synergy microplate reader (BioTek, USA) at Abs = 570 nm ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Immunology 2020Quote: ... NET formation was stimulated with 100nM PMA and detected with SYTOX™ Green nucleic acid stain (Thermo) at 528nm during 6 hr incubation at 37°c in a Synergy HT (BioTek) plate reader (as previously described).[31] NET formation was confirmed by fluorescence microscopy.
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bioRxiv - Microbiology 2022Quote: ... intracellular NR dye was extracted using a destaining solution (50% ethanol, 1% acetic acid and water) and absorbance was measured at 540 nm using a spectrophotometer (Biotek, Elx800). Percentage of inhibition of each drug was calculated normalizing data against the negative control absorbance (100% value).
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bioRxiv - Cancer Biology 2023Quote: ... The chromogenic reactions were stopped by adding 50 µL per well of 10% sulfuric acid (H2SO4) and absorbance values were measured by a Synergy H1 Hybrid microplate reader (BioTek, USA). Absolute IFNγ concentrations in test samples were interpolated from a standard curve and represented using GraphPad Prism v10 Software.
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bioRxiv - Cell Biology 2019Quote: ... The reaction was then stopped by adding 50 µl of 2N sulfuric acid and data were acquired by reading the absorbance at 405 nm using a Synergy H1 microplate reader (BioTek Instruments Inc.). To normalize to cell number ...
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bioRxiv - Immunology 2021Quote: ... reactions were stopped with 0.16 M sulfuric acid and absorbance was read spectrophotometrically at 450 nm using the ELx808™ Absorbance Microplate Reader (BioTek, Winooski, VT). End-point titers were determined and expressed as the reciprocals of the highest dilution with OD reading above the cut-off value defined as the mean of the control group plus three standard deviations (SD).
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 50μl/well of 2M sulphuric acid (H2SO4) and optical densities (OD) read at 490nm with a BioTek Synergy4 reader (BioTek Instruments, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped with 2M of sulfuric acid and absorbance measured at 450 nm in a Cytation5® plate reader (BioTek, Winooski, VT, USA). The performance of blank ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Immunology 2022Quote: ... Antibody levels are presented as optical density absorbance values at 450 nm (BioTek ELISA plate reader).
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bioRxiv - Cancer Biology 2022Quote: ... was added after secondary antibody incubation for signal development detected at 450nm using an Epoch 2 microplate spectrophotometer (BioTek Instruments). If the compound interferes with PYGO2-H3K4me2 binding ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody binding was quantified using the Pierce TMB Substrate Kit (Thermo, Cat. 34021) and Synergy HT plate reader (BioTek, Winooski, VT). Antibody binding data were analyzed by nonlinear regression (One site specific binding with Hill slope ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescence of dextran was measured at 490 nm excitation/520 nm emission and fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4). Clearance volume in UT and FUS+MB treated Transwells was calculated as previously described [30] and data presented as fold change to UT.
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...
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bioRxiv - Immunology 2023Quote: ... Milford MA) was added for antibody detection and the optical density at 450nm was read using Synergy LX Multimode reader (BioTek, Winooski, VT). The plasma bNAb concentrations were calculated from standard curves using the Gen5 Software (v ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...