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Citations for Biotek :
1 - 47 of 47 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Fluorescence polarization (FP) method was used to measure the binding affinity with Synergy 4 Microplate Reader (BioTek). Aliquots (5 nM ...
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bioRxiv - Molecular Biology 2020Quote: Fluorescence polarization (FP) method was used to measure the binding affinity using a Synergy 4 Microplate Reader (BioTek). Aliquots (5 nM ...
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Molecular Biology 2019Quote: ... and the kinetic curve was recorded using an enzyme-labeled instrument (Epoch, BioTek, USA) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... purified (EZNA Gel Extraction Kit, Omega Biotek), quantified (NanoDrop Technologies Inc. ...
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bioRxiv - Cancer Biology 2021Quote: Immunofluorescent labeled cells cultured in chamber slides were scanned using a Cytation 5 Imaging Multi-Mode Reader (BioTek). Cell nuclei were identified using DAPI and the abundance of γH2AX foci was determined using CellProfiler (v.4.1.3).
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were purified using Mag-Bind RXNPurePlus beads (OMEGA Biotek) (see Supplementary Information for preparation of magnetic bead size selection buffer ...
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bioRxiv - Microbiology 2023Quote: ... cDNA samples were purified using Totalpure NGS mag-bind (Omega-BioTek) at a 1.0x ratio (beads to cDNA volume) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
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bioRxiv - Bioengineering 2022Quote: ... 300 μL of the supernatant was removed and analyzed to determine the amount of FITC-labeled IgG using a Synergy H1 Hybrid Multi-Mode Plate Reader (BioTek). Release assays were conducted by adding 100 μL hydrogel to microcentrifuge tubes and then centrifuging until the hydrogel was situated in the bottom of the tube ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
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bioRxiv - Biophysics 2023Quote: All absorption and emission measurements for both labeled A2AAR and Rec were carried out at 25 °C on a Synergy H4 (Agilent BioTek) plate reader with Take3 Microvolume Plates ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments, Inc.) and confocal microscopy imaging respectively ...
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bioRxiv - Synthetic Biology 2021Quote: ... The approximately 8100bp amplicon was purified using a gel extraction kit (Omega Biotek). DNA concentration of both linear fragments was measured using the Take3 plate coupled with a Biotek Cytation 5 plate reader ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR products were purified using the EZNA Gel Extraction kit (Omega BioTek).
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bioRxiv - Microbiology 2022Quote: ... Libraries were purified using the Mag-Bind RXNPure plus magnetic beads (Omega Biotek) and finally ...
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bioRxiv - Synthetic Biology 2022Quote: The PCR2 products were purified using a 1.8x SPRI cleanup (Omega Biotek; M1378) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and purified by DNA purification and gel extraction kits (Omega Biotek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The pool was purified using the Mag-Bind RXNPure Plus magnetics beads (Omega Biotek), following the instructions provided by the manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then purified by DNA purification and gel extraction kits (Omega BioTek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Molecular Biology 2022Quote: ... and dsDNA fragments are extracted and purified from the gel (Omega Biotek, D2500-02). The purified DNA fragments are quantified by using a Nanodrop 2000 spectrophotometer.
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bioRxiv - Developmental Biology 2023Quote: Purified RNA was quantified and assessed for quality using Epoch 2 microplate spectrophotometer (BioTek). cDNA libraries were prepared from 500ng RNA per sample ...
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bioRxiv - Microbiology 2021Quote: ... The purified DNA samples were quantified on a microplate reader (Synergy LX, BioTek, Winooski, VT), and 500 ng of each DNA sample (20 ng/µl ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified from positive DH5α colonies with ENZA plasmid DNA mini kit (Omega Biotek) and submitted to Genewiz for sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... The product was purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). A second PCR reaction was used to add the index (for pooled sequencing ...
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bioRxiv - Microbiology 2023Quote: ... The pooled 16S amplicons were purified again using Mag-Bind magnetic purification beads (Omega Biotek) before denaturation of the libraries in preparation for loading into the Illumina V2 cartridge (2×250 bp paired-end reads ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA was purified using a Microelute RNA clean UP kit (Omega Biotek #6247-01). The following mixture was then prepared for injection into embryos ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were purified using the Mag-Bind RXNPure Plus magnetic beads (Omega Biotek, Norcross, GA, USA), following the instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... and mono-nucleosome bands were identified and selected under blue light and gel purified (Omega Biotek). Mononucleosomal DNA fragments were sequenced on an Illumina HiSeq 2500 instrument (2×125 paired-end sequencing) ...
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bioRxiv - Cancer Biology 2019Quote: ... 3µL of purified RNA was used for quantification using the Epoch plate reader (Biotek; Winooski, Vermont, USA). cDNA was made from purified total RNA using high capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... Samples were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were cloned into the pCR2.1 vector using the Original TA cloning kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The libraries were subsequently purified using Mag-Bind RXNPure Plus magnetic beads (Omega Biotek, Norcross, GA, USA), and then combined in equimolar amounts based on the quantification data obtained from the Qubit dsDNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Purified mAbs were analyzed for purity using 12% SDS-PAGE and concentration was determined by Take3 (BioTek instruments).
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bioRxiv - Immunology 2021Quote: ... Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Biochemistry 2020Quote: ... The −500 to +216 region corresponding to the HSPA1B promoter was amplified from the plasmid by PCR with Phusion polymerase (Thermo-Fisher #F530S) and purified via E.Z.N.A Gel Extraction Kit (Omega BioTek #D2500). The DNA was ethanol precipitated ...
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bioRxiv - Developmental Biology 2024Quote: ... and the band located at a 7.9 kb mark was cut and purified using the EZNA Cycle pure kit (Omega Biotek) Purified digested vector and inserts were ligated in a 1:30 vector/insert ratio using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was then purified according to the manufacturer’s instructions and quantified by absorbance on a BioTek Synergy H1 microplate reader (Biotek). Depending on the amount of RNA obtained ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5K cells/well in of purified OPCs were plated in PLO-laminin coated 384 well plates using the EL406 (BioTeK) peristaltic liquid dispenser ...
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bioRxiv - Biochemistry 2020Quote: ... Oligos were converted to double-strand DNA using qPCR and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). The pSC101 backbone was amplified with two separate primer pairs encoding BsaI cut sites that matched the insertion location of the oligos on the TtgR gene ...
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bioRxiv - Biochemistry 2022Quote: ... Oligonucleotides were converted to double-stranded DNA using 15 cycles of PCR amplification and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). A pSC101 backbone containing extant E ...
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bioRxiv - Immunology 2022Quote: ... and lambda chains.48 The products from the second PCR were analyzed by agarose gel electrophoresis and purified PCR products (ENZA cycle pure kit; Omega Biotek) were submitted to Genewiz for sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Purified total RNA was quantified on a Synergy 2 Multimode Microplate Reader using a Take3 micro-volume plate (BioTek, Winooski, VT). For cDNA synthesis ...
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bioRxiv - Immunology 2023Quote: ... The purified total RNA was eluted with 50 μL of elution buffer and quantified by absorbance using an Epoch spectrophotometer (BioTek, USA). Total RNA integrity was assessed by 1% agarose gel electrophoresis using 1× TAE buffer prepared with DEPC water.
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bioRxiv - Physiology 2019Quote: ... The final purified PCR products for use as templates for RNA probe synthesis were quantified on a SYNERGY 2 Microplate reader (Biotek, Winooski, VT).
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting was performed to separate purified human versus mouse cell fractions using the Mouse Cell Depletion Kit (Miltenyi Biotek, 130-104-694). For the proteomic experiments ...