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Citations for Biotek :
201 - 250 of 813 citations for 3 4 Ethoxy 3 methoxy phenyl 3 thiophene 2 carbonyl amino propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and tested for fluorescence intensity via a fluorescence micro-plate reader with the excitation wavelength of 488 nm and the emission wavelength of 525 nm (Cytation 3 Cell Imaging Multi-Mode Reader, Biotek, US).
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bioRxiv - Microbiology 2024Quote: ... the biofilms were stained with 0.1% crystal violet and OD590 was measured using a microplate plate reader (Cytation 3, BioTek or Clariostar) (23) ...
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bioRxiv - Immunology 2021Quote: ... Cell migration was assessed by a fluorescence (excitation at 485nm, emission at 530nm) plate reader Cytation 3 Cell Imaging Multi-Mode Reader (Biotek Instruments, Inc). The actual chemotaxis values were obtained by subtracting random chemotaxis values (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... was added to each well and the absorbance was measured at 340nM for the duration of the experiment by a multi-mode imaging reader (BioTek, Cytation 3).
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bioRxiv - Microbiology 2020Quote: ... Fluorescence (Ex = 335 nm and Em = 450 nm) was measured every minute on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of the extracted DNA was conducted spectrophotometrically with the Take 3 plate in an Eon plate reader/incubator (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... Fluorescence microscopy images (4X and 40X magnification) were acquired with the Cytation™ 3 Cell Imaging Multi-Mode Reader (BioTek® Instruments). Macrophage-residing HIV-1 in VCCs was analyzed with 40X magnification images ...
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bioRxiv - Bioengineering 2023Quote: ... It was then incubated for 1 hour at room temperature before the absorbance was read at 570 nm using a microplate reader (BioTek, Cytation 3). The manufacturer’s standard protocol was used to calibrate the measurements.
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken at 300 rpm (orbital) for 3 min and BRET biosensor performance was recorded using the plate reader’s luminescence filter (BioTek Synergy H1, v2.04.11) with an integration time of 1 s ...
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bioRxiv - Microbiology 2024Quote: ... to initiate reactions and then the fluorescence signal (λex= 335 nm and λem= 450 nm) was monitored by Cytation 3 Multi-Mode Reader (BioTek, Winooski, VT) at 25 °C for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence (λex = 380 nm, λem = 460 nm) of the AMC product was monitored by Cytation 3 Multi-Mode Reader (BioTek, Winooski, VT) (54 ...
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bioRxiv - Microbiology 2024Quote: ... Quantity and quality of the extracted DNA was determined with the Take 3 plate in an Eon plate reader/incubator (BioTek, Winooski, VT) (Culliney and Schmalenberger ...
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bioRxiv - Biochemistry 2020Quote: ... 30 μL of a 10 nM solution of FphF in PBS/0.01%TritonX-100 was added and fluorescence (λex = 365 nm and λem = 455 nm) was read at 37°C in 1 min intervals on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... The reduction of resazurin to resorufin was measured fluorometrically at 540 nm excitation and 590 nm emission in a Cytation 3 microplate reader (BioTek Instruments Inc., USA). The results were expressed as % of untreated cells ...
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bioRxiv - Microbiology 2020Quote: ... Em = 450 nm) over a period of 30-60 minutes at 37°C on a Cytation-3 imaging reader (BioTek, Winooski, VT, USA). Concentrations of hydrolyzed products were determined using a standard concentration curve (unconjugated 4MU) ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C and fluorescence was measured (Ex = 410 nm, Em = 450 nm) over a period of 60 minutes on a Cytation 3 imaging reader (BioTek, Winooski, VT, USA). Reaction rates were determined by linear regression analysis of the initial linear phase of hydrolysis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Relative dopaminergic cell viability was assessed by counting MAP2- and TH-positive neurons in a blinded manner using images taken with an automated Cytation 3 Cell Imaging Reader (BioTek Instruments, Winooski, VT) equipped with a 4X objective ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor simultaneously Cleavage was measured using fluorescence (EX 380 nm/EM 460 nm) with Cytation 3 imaging reader (BioTek, Winooski, VT, USA) for 60 minutes and slope was used to calculate the inhibition curve ...
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bioRxiv - Synthetic Biology 2023Quote: ... 80 µL ONE-Glo EX Reagent was then added and the plate was orbitally shaken at 300 rpm for 3 min in a microplate reader (BioTek Synergy H1, v2.04.11). Firefly luminescence was collected using the microplate reader’s luminescence fiber ...
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bioRxiv - Cancer Biology 2021Quote: ... was added to each well for 3 h and the absorbance at 450 nm was measured on an Epoch™ Microplate Spectrophotometer (Biotek, Winooski, VT, USA). Cytotoxicity data were expressed as mean ± standard derivation (SD ...
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bioRxiv - Cell Biology 2022Quote: ... 25 μL of xanthine 1x and the absorbance was read at 450 nm in a BioTek Cytation 3™ multiplate reader (BioTek Instruments Inc., USA). Results were expressed as % of the pool of controls.
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bioRxiv - Cell Biology 2024Quote: ... 100 µL of Tris (pH 10) was added and optical density was measured at 540 nm in Biotek Cytation 3 reader (Biotek Instruments, Winooski, VT, USA) and analyzed using the Wave Desktop 2.6.1 software (RRID:SCR_014526).
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bioRxiv - Bioengineering 2023Quote: ... We affixed the balloons to a glass microscope slide using tape and acquired images at 4x magnification with a Cytation 3 Image reader (BioTek Instruments Inc, Winooski, VT). All images were exported using a 16-bit resolution (65536 grey levels ...
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bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
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bioRxiv - Cancer Biology 2020Quote: ... was added and intensities were measured for each well using a Cytation 3 Multi Modal Reader with Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA). Along with cell viability ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence was measured at 340 nm excitation wavelength and 480 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent signal was measured at 548 nm excitation wavelength and 574 nm emission wavelength in a Cytation™ 3 microplate reader (BioTek Instruments Inc., Winooski, VT, USA). As a positive control ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and used to generate sequences with the desired amino acid substitution using Q5 Site-Directed Mutagenesis Kit (NEB, via BioTek, Praha, Czech Republic). Primers were designed using NEBaseChanger (https://nebasechanger.neb.com/) ...
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bioRxiv - Molecular Biology 2021Quote: ... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... removal by vegetative cells was measured by sampling supernatant and assaying AcA using the change in absorbance at 380 nm in the presence of 2--amino benzamidoxime (ABAO), (as inspired by aldehyde quantifications by Ressmann et. al, 2019) using an Epoch microplate reader (BioTek; Gen5 v3.10 software) [42] ...
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bioRxiv - Microbiology 2020Quote: ... the reaction was stopped with 50 μls of 3M hydrochloric acid and absorbance was measured at 490 nm (OD490) using a Synergy 4 (BioTek) plate reader ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were quenched with 2 M sulfuric acid before determining absorbance at 450 nm using a Synergy BioHTX plate reader (BioTek). The background value from wells containing no serum was averaged and subtracted from all wells on the plate.
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bioRxiv - Cancer Biology 2024Quote: ... the staining was eluted with 10% acetic acid solution and the optical density was measured at 570 nm in the spectrophotometer Epoch 2 (Biotek).
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... Culture OD600 was recorded every 4 minutes over 24 hours in an Epoch 2 plate reader (BioTek) set to 300 rpm and 30°C.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Cell Biology 2021Quote: ... Changes in intracellular Ca2+ concentrations were measured by recording the ratio of fluorescence intensities at 508/20 nm resulting from excitation of Fura-2 AM at 340/11 nm or 380/20 nm (center/bandpass) using a Synergy 4 multimode microplate reader (BioTek). Ratios were acquired every 0.7 seconds for 15 seconds before injection and 2 minutes after injection ...
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bioRxiv - Microbiology 2023Quote: ... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).
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bioRxiv - Cell Biology 2020Quote: Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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bioRxiv - Synthetic Biology 2023Quote: ... The absorbance at 405 nm was measured every 1 min for at least 30 min and up to 2 h using a Synergy 4 microplate reader (Biotek, Winooski, VT). SEAP activities were calculated as a function of the increase in absorbance at 405 nm over time42.
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bioRxiv - Immunology 2021Quote: ... Synergy 4 (BioTek), at an absorbance of 490 nanometers ...
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bioRxiv - Neuroscience 2024Quote: ... Synergy 4 (BioteK) plate reader was used to determine the concentration of LCN2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 images at the central callus and distal callus regions were taken using an automatic microscope (Agilent, BioTek Lionheart FX, Santa Clara, CA). Central callus regions were defined as proximal to the fracture site on either side of the bone ...
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bioRxiv - Cell Biology 2021Quote: ... Synergy 2 (BioTek).
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bioRxiv - Microbiology 2022Quote: ... measuring luminescence with Synergy 4 (Biotek). 1% of Triton-X100 and 0.25% DMSO were used as the positive and negative control ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acid concentration was measured using a microplate reader Synergy HT (Biotek, Germany), with the software Gen5™ (Biotek ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... with the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The nucleic acid concentration was determined using a microplate reader (Synergy H1; BioTek), and the quality of the RNA was assessed using Agilent 2100 Bioanalyzer system (Agilent Technologies) ...