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Citations for Biotek :
851 - 900 of 987 citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Fluorescence intensity (F) and the final OD600 of each sample was recorded on a Synergy 2 Multi-Mode Microplate Reader (BioTek) using excitation and emission filters with wavelengths of 485±20 nm and 535±20 nm ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were quenched with 2 M sulfuric acid before determining absorbance at 450 nm using a Synergy BioHTX plate reader (BioTek). The background value from wells containing no serum was averaged and subtracted from all wells on the plate.
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bioRxiv - Microbiology 2023Quote: ... Bacterial growth was monitored during incubation at 30°C in microtiter plates in an Epoch 2 or Synergy H1M plate reader (Biotek).
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bioRxiv - Microbiology 2023Quote: ... by pipetting 250 µL and using a Biotek® Epoch™ 2 multi-well plate reader (Biotek Instruments, Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... then diluted to A600=0.1-0.001 into either 5-10mL broth or into 96-well plates (Costar), and growth was monitored by measuring A600 every hour (broth culture) or every 15min (Epoch 2 or SynergyMx, (Biotek) plate readers. ...
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bioRxiv - Microbiology 2024Quote: ... The area under the curve of kinetic substrate conversion curves over 2 hours was measured with a Synergy H1 plate reader (BioTek) and normalized to uninfected controls.
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bioRxiv - Microbiology 2024Quote: ... Culture growth was measured using chlorophyll a autofluorescence (excitation/emission: 440/680 nm) as a proxy for biomass using a Synergy 2 microplate reader (BioTek).
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bioRxiv - Bioengineering 2024Quote: ... The absorbance was measured at 450 nm for each sample and compared using a fluorescence microplate reader (BioTek, Synergy 2).
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bioRxiv - Bioengineering 2024Quote: ... The final co-cultures were then incubated at 37°C in an automated spectrophotometer (Biotek Synergy 2, Agilent Technologies, USA) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... was added to each well and after 2 hours in the incubator absorbance was measured using a microplate reader (BioTek, Synergy HT ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescence changes at excitation/emission of 535/595 nm for JC-10 aggregates and at 485/535 nm for JC-10 monomers were monitored with a Synergy 2 Reader (BioTek). Mitochondrial membrane potential was quantified as the fluorescence of JC-10 aggregates/monomers (595/535 nm).
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bioRxiv - Microbiology 2024Quote: ... subtilis pyruvate kinase activities in substrate and activator combinational titrations are conducted in 96 well plates and the absorbance at 340 nm was monitored by a Synergy 2 microplate reader (BioTek). The reaction mix contained 100 mM Tris-HCl (pH=7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 % (0.25 mg/mL) were placed in a 96-well microtiter plate and incubated in a Synergy 2 Multi-Mode Reader (BioTek) at 37°C under continuous shaking during 40 min ...
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bioRxiv - Bioengineering 2024Quote: ... The plate was then quickly spun down at 3000 rpm and scanned from 510 to 580 nm nm in increments of 2 nm on Synergy Neo2 Multi-Mode Plate Reader (BioTek). Values reported are averages of duplicate wells and are sample red shift wavelengths at maximum absorbance subtracting the blank reference (PBS only) ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescence increase resulting from degradation of peptide-AMC at 37 °C was monitored over time by means of a fluorometer (Synergy 2 Hybrid Multi-Mode Microplate Reader, BioTek) at 360-nm excitation and at 460-nm emission ...
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bioRxiv - Cancer Biology 2024Quote: ... the staining was eluted with 10% acetic acid solution and the optical density was measured at 570 nm in the spectrophotometer Epoch 2 (Biotek).
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in 4% PFA and stained with Hoechst nuclear stain before GFP-positive cells were counted using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) with the onboard software for calculations of percentage infected cells.
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bioRxiv - Microbiology 2020Quote: ... Infected cells were then maintained at 37°C for 14-16 h post infection before automatic counting of GFP-positive cells using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) and using the onboard software for calculations of percentage infected cells.
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bioRxiv - Cancer Biology 2021Quote: ... at 1.5×104 cells/ well in 50 μl of DMEM supplemented with 10% FBS using BioTek™ MultiFlo™ FX Microplate Dispensers (BioTek Instruments). Cells were treated with an FDA approved library (Selleckchem ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was quantified using the Take3 microvolume plate accessory on a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States), RNA purity was determined by analyzing A260/280 and A260/230 ratios ...
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bioRxiv - Plant Biology 2020Quote: ... Fluorescence intensity was measured at the vascular bundles of leaves 5 mm from the detachment sight using the Lionheart FX Gen5 image analysis mode (BioTek, Winnoski, VT, USA). Untreated plants were used as control with 6 biological repeats ...
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bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... isopropyl alcohol to quantitatively measure the density of the stain in different groups at 520 nm using microplate reader (Gene 5 Bio Tek Spectrometer, BioTek, northern Vermont, USA). Isopropanol was used as a blank (26)
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bioRxiv - Neuroscience 2020Quote: ... Immunofluorescence images were acquired using a Cytation 5 Cell Imaging Multi-Mode Reader and analyzed with Gen5™ software (BioTek, Winooski, VT, USA).
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bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
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bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). 108 colony-forming units (CFUs ...
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bioRxiv - Microbiology 2023Quote: ... ODs were obtained by measuring absorbance at 600nM in a 96-well plate using Cytation Station 5 (BioTek Agilent Technologies, Santa Clara, CA). The liquid cultures were centrifuged at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor ...
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bioRxiv - Cancer Biology 2022Quote: ... The fraction of cells surviving was quantified using either a MacsQuant Analyzer 10 (Milltenyi Biotec, Germany) or by the Cytation 5 system (BioTek, Santa Clara, California). Cell viability was measured according to the manufacturer’s instructions for both instruments ...
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bioRxiv - Biophysics 2022Quote: ... 100 μL of CellTiter-Glo 2.0 was added to each well and luminescence signal was measured in a Cytation 5 imaging reader (BioTek instruments Inc., Winooske, USA) after 10 min incubation.
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
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bioRxiv - Cancer Biology 2024Quote: ... were seeded in 384-well plates in 25 µl of respective culture media using BioTek MultiFlo FX RAD (5 µl cassette) (Biotek, Winooski, VT, USA). Indicated concentrations of small molecules were added for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... the absorbance of each well at 540 nm was measured using a Cytation 5 cell imaging multimode reader (Agilent Biotek, Santa Clara, California). The mean absorbance values were used for the calibration curve and the samples ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by detection of the absorbance at 420 and 550 nm at every 2 min using the Viento Nano microplate reader (BioTek Instruments). The relative LacZ activity was calculated as follows ...
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bioRxiv - Physiology 2020Quote: ... The samples were incubated 37 °C for 2 hours and absorbance was measured at 562 nm using a microplate spectrophotometer (BioTek Epoch).
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bioRxiv - Biochemistry 2021Quote: ... The released pNP (extinction coefficient ε=16,000 M-1cm-1) was measured at 405 nm using Epoch 2 plate reader (Biotek, US) set to 37°C and subtracting the blank reading ...
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bioRxiv - Microbiology 2020Quote: ... Promoter activity was then followed by continuous measurement of the growth and GFP fluorescence (one measure every 30 min during 10 h) of the cultures with the Synergy™2 Multi-detection microplate reader (Biotek). Promoter activity was then estimated by dividing the fluorescence value by the OD600 value for each time point.
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bioRxiv - Physiology 2021Quote: ... A 1.5 h kinetics curve was monitored at 37° using a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT, USA). The absolute amount of cleaved substrate was calculated by an AMC standard curve ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped with 50 µL of 2N sulfuric acid and the absorbance at 450 nm was read with an Epoch 2 microplate reader (Biotek, USA).
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bioRxiv - Neuroscience 2021Quote: ... Purified total RNA was quantified on a Synergy 2 Multimode Microplate Reader using a Take3 micro-volume plate (BioTek, Winooski, VT). For cDNA synthesis ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNA concentration and purity was monitored by electrophoresis on 2% (w/v) agarose gels and spectrophotometrically by determination of A260/A280 ratios (Synergy HT Microplate Reader, BioTek, USA). Amplicon based metagenomics was performed using the NEBNext® Ultra™ IIDNA Library Prep Kit (Cat No ...
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bioRxiv - Immunology 2020Quote: ... The reaction was stopped with stop solution and absorbance was read at 450 nm using Epoch 2 microplate reader (BioTek, USA). Data are expressed as stimulation index (S.I ...
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bioRxiv - Neuroscience 2021Quote: ... Relative Light Units (RLU) were measured at 28°C using the Synergy 2 SL Microplate Reader with Gen5™ software (BioTek).