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Citations for R&D Systems :
1 - 49 of 49 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... human CXCL1/GROα duo-set or CCL5 duo-set or IGFBP3 duo-set (R&D Systems) were used to detect the concentrations of CXCL1 or CCL5 or IGFBP3 in the plasma following manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... human CXCL1/GROα duo-set or CCL5 duo-set or IGFBP3 duo-set (R&D Systems) were used to detect the concentrations of CXCL1 or CCL5 or IGFBP3 in the CM following manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Duo Set ELISA kits (R&D Systems) were used for IL36α ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR analysis of pluripotency markers was done using the Human Pluripotent Stem Cell Assessment Primer Pair Panel (R&D Systems, SC012) and amplification was done using GoTaq Green ...
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bioRxiv - Immunology 2020Quote: ... by ELISA using Duo-Set kits (R&D Systems), according to the supplier’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... human GDF-15 Duo Set (R&D Systems, cat.# DY957), human Serpin E1/PAI-1 Duo Set (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... human Serpin E1/PAI-1 Duo Set (R&D Systems, cat.# DY1786), and human total MMP-3 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... plasma was tested with mouse Flt3L ELISA Duo Set from R&D systems. For human IL-6 ...
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bioRxiv - Developmental Biology 2023Quote: ... set up in either vehicle or with BMP4 (314-BP, R&D Systems) and cultured on a transwell insert in a 6-well plate in DMEM with 10% FCS ...
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bioRxiv - Immunology 2019Quote: ... We established in vitro quantification of granzyme B (antibody sets from R&D Systems) and perforin (antibody sets from MABTECH) ...
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bioRxiv - Immunology 2019Quote: ... IL-1β and IL-6 were detected by ELISA duo sets (R&D Systems) following the manual ...
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bioRxiv - Immunology 2021Quote: ... Commercial DuoSet matched-antibody reagent sets were purchased from R&D Systems (Minneapolis, MN) for quantifying human IL-6 and human TNFα and used according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IL-6 and IL-8 was measured using duo set ELISAs from R&D Systems according to manufactures instructions.
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bioRxiv - Cell Biology 2023Quote: ELISA was performed using the human IL-6 Duo-Set (R&D Systems, cat.# DY206), human GDF-15 Duo Set (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... IL-12p40 cytokine release was quantified using the Duo Set ELISA kit from R&D Systems according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl plasma from each mouse was tested using the mouse CXCL1/KC duo-set (R&D Systems). The experiments were done following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... incubated 1 h in RT with HRP-conjugated secondary antibodies (R&D Systems), and followed by rinsing 3x 5 minutes with TBS-T ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... IL-10 and IL-6 were measured by sandwich ELISA using the Duo Set ELISA kit (R&D Systems, UK) (23 ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were activated in the presence of the following sets of cytokines and/or neutralizing antibodies (all from R&D Systems): Th0—media alone ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg total protein was collected from each treated kidney and IL6 protein level was determined by the mouse IL6 duo-set (R&D Systems) ELISA ...
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bioRxiv - Microbiology 2019Quote: ... Cytokine concentrations in cell-free lung homogenates were quantified using commercial enzyme-linked immunosorbent assay (ELISA) kits (IFNγ Duo Set #DY485 R&D Systems) according to manufacturer instructions.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Threshold gates were set with the same samples that were stained with the following isotype control antibodies: Normal chicken IgY Control (R&D Systems), and normal rabbit IgG control (Santa Cruz Biotechnology) ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine levels were assessed in the BAL with Duo-Set Enzyme-Linked Immunosorbent Assays (R&D Systems Inc., Minneapolis, MN). ELISAs were performed per manufacture’s instructions.
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bioRxiv - Microbiology 2022Quote: Distal ileal TNF-α and IL-6 were quantified using the mouse TNF-α enzyme-linked immunosorbent assay [ELISA] Ready-SET-Go kit (eBioscience; San Diego, CA, USA) and the Mouse IL-6 DuoSet ELISA (R&D Systems) respectively ...
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bioRxiv - Immunology 2024Quote: ... IL-1β and IFN-β were measured using mouse R&D Duo set ELISA kits according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... Enzyme-linked immunosorbent assay for human LTα was performed in triplicate using the DUO set ELISA kit (DY210, R&D Systems, Abingdon, UK). Protein was extracted from transfected meningeal cells using RIPA buffer (R0278 ...
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bioRxiv - Microbiology 2021Quote: The secreted TNF-α and IL-6 cytokines in the culture supernatants were quantified by ELISA using Duo Set ELISA (R&D systems) as per the manufactures instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The frozen cortex was homogenized according to the manufacturer’s of the Duo set IC active PP2A kit (reference DYC3309, R&D systems, Minneapolis, USA) and has been previously described (Gratuze et al ...
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants or sulcus fluid from patients with different disease stage were evaluated for MMP-9 content using ELISA Duo Set (DY911, R&D System, Wiesbaden, Germany.) following the manufactureŕs protocol.
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bioRxiv - Immunology 2022Quote: IL-10 and TNF-α levels were measured in the cell-free supernatants of cell cultures using a cytokine Duo-Set Kit (R&D Systems, Minneapolis, MI, USA), according the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at RT with anti-PAR/pADPr monoclonal antibody (R&D Systems cat# 4335-MC-100) diluted 1:250 in 3% BSA-PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated for 2 h at room temperature (RT) with biotinylated polyclonal goat anti-human TREM2 capture antibody (0.1 μg/ml; AF1828, R&D Systems). Following incubation with streptavidin-HRP (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Retinal organoids on day 90 of differentiation were incubated at RT for 1 hour in PBS with either a goat anti-ACE2 antibody (AF933, R&D Systems) or a normal goat IgG control (AB-108-C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked in PBS and Tween-20 with 1 % (w/v) nonfat dry milk for 1 h at RT followed by incubation with 1:50 PAX6 (AF8150; R&D Systems) or 1:500 FOXC1/2 (CPA3482 ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 1μg of mouse serum IgG for 10 min at RT then incubated with: AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), APC mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatant was collected and centrifuged (3000 rpm, 5 min, RT) and Secreted IL6 was measured using the Quantikine® ELISA kit (R&D Systems), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...