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1 - 11 of 11 citations for Fluorescein alkynylamino atp since 2020

• Structures of BIRC6-Client Complexes Provide Mechanism of Smac-Mediated Release of Caspases

  Moritz Hunkeler, Cyrus Y. Jin, Eric S. Fischer, bioRxiv - Molecular Biology 2022

  Quote: ... Mg-ATP (R&D systems, B20) at 1 mM ...
• Clr4^SUV39H1 and Bdf2^BRD4 ubiquitination mediate transcriptional silencing via heterochromatic phase transitions

  Hyun-Soo Kim, et al., bioRxiv - Molecular Biology 2021

  Quote: ... Mg-ATP (R&D systems B-20) and 10x E3 Ligase Reaction Buffer (R&D systems B-71) ...
• Gram-negative bacterial infection increases lung cancer metastasis via Toll-like receptor activation and increased cancer cell proliferation post-tumor adhesion

  Roni F. Rayes, et al., bioRxiv - Cancer Biology 2021

  Quote: Fluorescein-conjugated monoclonal rat anti-mouse TLR2 IgG2a (R&D systems (Minneapolis, MN)) was used at 50 μg/mL ...
• IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota

  Ivo Röwekamp, et al., bioRxiv - Immunology 2023

  Quote: ... ATP and IL-33 were measured via ELISA (eBioscience, R&D Systems) according to the manufacturer’s instructions.
• Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains

  Kaitlin S. Radmall, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 5 mM ATP) with 0.1 μM recombinant yeast E1 (R&D Systems), ...
• Identification of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme

  Cameron Smith, et al., bioRxiv - Cell Biology 2024

  Quote: ... 100 nM HsUBA1 (E1) and 10 mM ATP in ubiquitin conjugation reaction buffer (R&D systems).
• PYK2 controls intestinal inflammation via activation of IRF5 in macrophages

  Grigory Ryzhakov, et al., bioRxiv - Immunology 2020

  Quote: ... Viability of intestinal biopsies was measured by TACS® TdT in situ (Fluorescein) TUNEL assay (#4812-30-K, R&D systems) according to manufacturer’s instructions ...
• Gram-negative bacterial infection increases lung cancer metastasis via Toll-like receptor activation and increased cancer cell proliferation post-tumor adhesion

  Roni F. Rayes, et al., bioRxiv - Cancer Biology 2021

  Quote: ... fluorescein-conjugated monoclonal rat IgG2a and fluorescein-conjugated monoclonal rat IgG2b were used at identical concentrations as isotype controls (all from R&D systems). All antibodies were incubated for 1hr with cells in 0.1M Tris-HCl ...
• Identification of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme

  Cameron Smith, et al., bioRxiv - Cell Biology 2024

  Quote: ... , and 5 uM of PfHEUL (PF3D7_0826100) HECT domain were incubated with 10 mM ATP in ubiquitin conjugation reaction buffer (R&D systems). All reactions were incubated at 37C for 1 hour ...
• Valosin-containing protein regulates the stability of amyotrophic lateral sclerosis-causing fused in sarcoma granules in cells by changing ATP concentrations inside the granules

  Kyota Yasuda, et al., bioRxiv - Cell Biology 2021

  Quote: ... Cells in ATP depletion were prepared by culturing the cells in a medium containing 100 μM CCCP (R&D systems, Minneapolis, MN) and 600 μM 2-deoxy-D-glucose (2DG ...
• Maternal separation stress experienced by parents affects ovarian function in first generation of mice

  Kajal Khodamoradi, et al., bioRxiv - Cell Biology 2020

  Quote: ... The level concentrations of IL-18 and IL-1β in ovarian tissue were measured using ELISA kits (Koma Biotech–Korea) and the level concentrations of ATP and GPx were measured with specific ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocols.