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Citations for R&D Systems :
251 - 300 of 1048 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Immunology 2020Quote: ... supernatants were collected and secretion of TGF β and MMP2/3/9 was assessed in the supernatant by TGFβ1 ELISA kit (R&D systems) or MMP2/3/9 luminex kit (eBiosciences ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Microbiology 2020Quote: ... hESCs were cultured to achieve 80-90% confluency, and treated with 3 μM CHIR99021 (CHIR, Stem-RD) and 100 ng/ml Activin A (R&D systems) in basal medium RPMI1640 (Cellgro ...
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bioRxiv - Systems Biology 2021Quote: ... HGF 50 ng/ml; NT-3 10 ng/ml; PDGF-BB 10 ng/ml; GDNF 10 ng/ml; All from R&D systems). Ligand treatment was started at the time of tetracycline induction ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative cytokine content of the decellularized mouse heart tissues (n=3) were obtained using the Mouse XL Cytokine Array Kit (ARY028, R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Pathology 2020Quote: ... was measured immediately before injection and at 3 days after injection using a Mouse Lipocalin-2/NGAL Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems) to ensure successful injection of folic acid ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC at 60-70% confluence were treated for 3 days with 100 ng/mL Activin A and 100 ng/mL bFGF (R&D Systems) in the presence of increasing levels of FBS (0% on day 1 ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... one million cells were suspended in 100 µl of 1:1 RPMI and BME-Type 3 (R&D Systems, # 36-320-1002P) per implantation ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: goat anti-Human/Mouse Caspase-3 (R&D Systems, Cat# AF-605-SP, 1:100-1:200), guinea pig anti-CNP1 (Synaptic Systems ...
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Bioengineering 2024Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...