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Citations for R&D Systems :
101 - 126 of 126 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Immunology 2024Quote: ... For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050, R&D Systems) at 1 µg/ml.
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... ex vivo lamina propria lymphocytes were cultured for 20 h at 37°C in complete RPMI with IL-23 (25 ng ml−1 R&D Systems) and IL-1β (20 ng ml−1 Peprotech ...
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bioRxiv - Immunology 2024Quote: ... 5 × 105 splenocytes were isolated from each group and incubated with 2 × 105 antigen stimulated BMDC and 2 µg/mL antigen for 20 h in the ELISPOT plate (R&D systems). ELISPOT measurement and analysis were conducted according to the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... The neutralizing capacity of the αVEGF-B monoclonal antibody was evaluated by stimulating confluent HUVECs pre-starved for 2 h in GFD media with a fixed amount of VEGF-B186 (50 ng/ml; R&D Systems) for 2 h in GFD media ...
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bioRxiv - Cancer Biology 2024Quote: ... Kuramochi) was evaluated 24 h after changing the medium using the Human IL-6 DuoSet ELISA Kit (DY206-05, R&D Systems) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: CCL3 and MPO concentrations were detected in immune cell supernatants after 24 h stimulation using Mouse CCL3/MIP-1 alpha DuoSet ELISA and Mouse Myeloperoxidase DuoSet ELISA kits (both R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Supernatants of three biological replicates were harvested 24 h after medium change and Proteome Profiler Human XL Cytokine array (R&D Systems) analysis was performed following the manufacturer’s protocol and signals were detected ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies consisted in the human anti-spike mAb 48 (1:1,000; a gift from H. Mouquet) or the mouse anti-p24 Gag MAB7360 (R&D Systems; 1:1000). Anti-human or mouse IgG secondary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
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bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
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bioRxiv - Immunology 2023Quote: Cytokine levels of TNFα was quantified in serum obtained from mice infected with and without Salmonella for 24 h by the mouse/rat TNF-A Valukine ELISA kit (R&D Systems, #VAL609) following the product protocol.
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bioRxiv - Biochemistry 2024Quote: ... cells were washed twice with cold FACS buffer and consequently incubated with anti-mouse F(ab)2 IgG (H+L) PE-conjugated antibody (R&D systems, F0102B) for 1h in the dark at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... fibroblasts were serum starved for 16 h and then treated with BMP2 and BMP4 (#355-BM-010 and #314-BP-010, R&D Systems, Minnesota, USA) or BSA at a concentration of 50 ng/mL of each ligand for 12 h ...
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bioRxiv - Microbiology 2024Quote: ... The UCP conjugates for chemokine monokine induced by gamma interferon (MIG) and complement factor H (CFH) were prepared with mouse anti-human MIG (49106, R&D systems, Minneapolis, USA), and mouse anti-human CFH (MAB4779 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytokines released from HCEC-1CT cells into cell culture supernatants after 5 h of incubation were measured using a Magnetic Luminex® assay (R&D SYSTEMS, Minneapolis, USA). Cell culture supernatant samples were collected after the incubation for cytokine gene transcription (details for incubation see 2.4. ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse SCLC cells were seeded into 6-well plates and cultured for 16 h in RPMI1640 + 10% BGS with or without 100 ng/mL of Wnt5a (R&D System, 645-WN-010) or recombinant Wnt3a (PeproTech ...
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bioRxiv - Microbiology 2022Quote: ... 5×105 isolated splenocytes were cocultured with different stimuli in 200 μL of RPMI-10% FBS complete medium for 48 h on IFN-γ-coated plates (Cat# EL485, R&D systems, Minneapolis, USA). Fifteen-mer overlapping peptides from SARS-CoV-2 spike glycoprotein (Cat# PM-WCPV-S-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... was assessed on 5μL murine plasma using the human SimplePlex Assay as the kit is cross reactive with murine NF-H (R&D Systems, Protein Simple SPCKB-PB-000519) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... SH-SY5Y and SK-N-AS cells were pre-treated for 16 h with 1000 Units/ml Interferon Type 1 (Interferon α/β, R&D Systems (Minneapolis, MN, USA)) followed by 2,5 μM BV6 (Selleckchem ...