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Citations for R&D Systems :
1051 - 1100 of 1363 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Physiology 2024Quote: ... the platelets were incubated at 4°C over night with antibodies against CXCR4 (MAB172, Clone # 44716, R&D Systems, Minneapolis, Minnesota, USA), ACKR3 (ab72100 ...
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bioRxiv - Neuroscience 2024Quote: ... All the sections were incubated overnights at 4 °C with primary antibodies including: rat anti podocalyxin (1:400, MAB1556, R&D Systems), goat anti PDGFRβ (1:250 ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 1 h with 5% dried skim milk in TBS containing 0.1% Tween 20 (T-TBS) and incubated overnight at 4°C with specific primary mAbs and pAbs: Nluc (mouse mAb; R&D Systems) for Nluc-expressing viruses ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Physiology 2024Quote: ... in 0.1% Tween-20 in PBS and incubated in the following antibodies overnight at 4°C: Goat anti-GLI3 (1μg/ml, R&D Systems, #AF3690) or Rabbit anti-alpha-tubulin (1μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ml of serum (collected and combined from n=4 mice) was used to probe the Mouse Cytokine Proteome Profiler Array (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... All of the experiments with MEFs were performed at passage 4 (P4) and at 60-70% confluence on glass fibronectin (1030-FN-05M, R&D Systems) coated coverslips or plastic culture plates (Starlab).
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bioRxiv - Cell Biology 2024Quote: ... TGF-β1 was reconstituted in sterile 4 mM HCl + 0.1% BSA according to manufacturer’s instructions (R&D Systems Cat #240-B-002). Indicated conditions were treated with the TGF-β1 inhibitor SB 431542 (Sigma-Aldrich Cat# S4317 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were embedded in Cultrex® RGF BME Type 2 (cat no. 3533-005-02, R&D systems) and placed in a 37°C incubator for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... and FABP-2 were measured from plasma using standard ELISA kits from R&D Systems (DC140, DC1630 &DFBP20). Positive testing controls for each ELISA kit were also included (R&D Systems QC20 ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... or recombinant human cathepsin L (CatL, 2 ng/ul, pH 5.5, 37°C for 1h; R&D Systems) as described previously (5) ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tumors were homogenized with a tissue homogenizer in 700μL of Cell Lysis Buffer 2 (R&D Systems) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: Mature adipocytes were cultured in complete medium supplemented with AZA (R&D Systems; 5, 10, 20µM; 2 days) and rhPDGF-AB (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 mM sodium pyruvate and 0.1 mM 2-mercaptoethanol) containing 50 ng/ml of ACTA (R&D Systems), 3 μM CHIR99021 (Tocris Bioscience ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Cancer Biology 2022Quote: For recombinant E6 proteins and sdAbs: 2 µg of E6 (R&D Systems Inc., Cat. # AP-120-025), MBP (Abnova ...
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bioRxiv - Cell Biology 2020Quote: ... to RAW264.7 or 2 ng/mL transforming growth factor β-1(240-B/CF, R&D System, USA) to THP-1 for 24-36 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were grown in DMEM/F12 (HyClone, SH30023.02) supplemented with 2 %v/v N21 (R&D system, AR008), 20 ng/mL EGF (R&D system ...
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bioRxiv - Immunology 2023Quote: ... we detected C5a with 2 μg/ml anti-C5a detection antibody (C5a DuoSet ELISA kit, R&D systems), followed by an incubation of 1:5000 streptavidin-HRP (Southern Biotech) ...
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bioRxiv - Immunology 2023Quote: About 200 colonic crypts were seeded in 25 μL Cultrex BME Type 2 (R&D systems, Minneapolis, MN) domes and cultured in Advanced DMEM/ F-12 supplemented with N2 ...
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bioRxiv - Neuroscience 2022Quote: ... Human TDP-43 DuoSET ELISA kit DuoSet Ancillary Reagent Kit 2 (#DY008, R&D Systems, Minneapolis, MN, USA), and tetraspanin exosome marker CD81 (CSB-EL004960HU ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: ... all necessary diluents and buffers were from the DuoSet® Ancillary Reagent Kit 2 (R&D Systems, #DY008). The assays were performed at RT unless otherwise noted ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA), and in the presence or the absence of CH-223191 or FICZ ...
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bioRxiv - Cancer Biology 2022Quote: ... Control or shLATS1/2 cells were counted and resuspended in 1% methylcellulose medium (R&D system Cat. # HSC001) prepared in complete growth medium ...
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bioRxiv - Immunology 2023Quote: ... Using a SARS-CoV-2 spike RBD mAb of known concentration (MAB10580; R&D Systems, Minneapolis, Minnesota, USA) as a molecular standard ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were stimulated with 2 ng/mL recombinant human TGF-β1 (R&D Systems, cat. # 240-B) diluted in starvation medium for varying durations of 30 ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured in RP10 supplemented with 300 IU/ml recombinant human IL-2 (R&D Systems) for 72 h unless otherwise noted ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets from the peritoneal lavage and bone marrow were lysed using Lysis 2 buffer (R&D systems), containing HALT protease inhibitors (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... were washed in 1X PBS then incubated in goat anti-GAS1 (2 mg, R&D Systems; Cat#AF2644) antibody diluted in 1X PBS for 2 h at room temperature on a rotating platform ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with human IL-13 R alpha 2 antibody (AF146, R&D Systems, Minneapolis, MN, USA) followed by donkey anti-goat IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were split 1:2 in SF-D media containing rhVEGF (5 ng ml-1; R&D Systems), rhBMP4 (10 ng ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Physiology 2020Quote: ... non-esterified fatty acid (NEFA) serum levels were measured by colorimetric assay (Randox, UK) and dipeptidyl peptidase 4 (DPP4; R&D Systems, UK) levels were measured by ELISA.
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
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bioRxiv - Microbiology 2021Quote: Sterile tissue culture plates were coated overnight at 4°C with 5 µg/mL recombinant CD62P protein (R&D Systems, #137-PS-050). The following day the wells were washed 3X with PBS before being blocked with 1% BSA in PBS at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were fixed in 4% paraformaldehyde solution as previously described.39 Membranes were immunoblotted for AXL (AF154 R&D Systems at 1:500), MerTK (ab52968 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked in 3% bovine serum albumin for 30 min and incubated with the following primary antibodies overnight at 4°C: β-III tubulin (1:1000, MAB1195, R&D system), rabbit anti-MAP2 (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.