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Citations for Miltenyi Biotec :
1 - 41 of 41 citations for Toxoplasma gondii IgG antigen RH strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml rh interleukin (IL)-4 (130-093-921, Miltenyi Biotec)] and cultured at 37°C ...
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bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
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bioRxiv - Immunology 2020Quote: ... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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bioRxiv - Immunology 2019Quote: ... antigen-specific T cells were separated using the CD154 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s suggestions ...
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bioRxiv - Microbiology 2020Quote: ... αhuman-IgG-PE (Miltenyi Biotec). αHis-PE (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... but from the ancestral strain (PoolRef) (all pools 1 μg/ml each, Miltenyi Biotec). After 18 hours ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG MicroBeads (Miltenyi Biotec), and LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... and IgG APC IS11-3B2.2.3 (Miltenyi Biotec). Data of labelled cells were acquired using a Beckman Coulter Gallios instrument and FlowJo ™ software (BD ...
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bioRxiv - Immunology 2020Quote: ... anti-mouse IgG magnetic beads (Miltenyi Biotec) were added ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8 T cells were isolated from the spleen of pmel mice (having CD8 T cells bearing T cell receptors specific for the gp100 antigen) by magnet-assisted cell sorting (Naïve CD8+ T cell isolation kit, Miltenyi Biotec) and stained with Cell Trace Violet (CTV ...
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and suspensions were incubated with magnetic microbeads targeting the uNK specific antigen CD56 (#130-050-401, Miltenyi Biotec, Bergisch Gladbach, Germany) diluted 1:20 in wash buffer (1% BSA/PBS ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Immunology 2024Quote: ... Purified CD154+ T cells were expanded in presence of 1:100 autologous antigen-loaded irradiated feeder cells in TexMACS medium (Miltenyi Biotec), supplemented with 5% (v/v ...
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bioRxiv - Immunology 2020Quote: ... anti-igG-APC (Miltenyi Biotec, 130-119-772), anti-CD27-Brilliant Violet 605 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... On day4 transduced cells were positively selected by magnetic assisted cell sorting (MACS) via the cell surface antigen CD90.1 (Thy1.1) using microbeads (Miltenyi Biotec: 130-094-523); the manufacturer’s protocol was adjusted by using 4x less reagents and by maintaining cells at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... and subsequently with anti-rabbit IgG MicroBeads (Miltenyi Biotec), and for the PM purification ...
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bioRxiv - Immunology 2020Quote: ... rat IgG 2b k-PE (Miltenyi Biotec, Clone: ES26-5E12.4); armenian hamster IgG-APC (eBioscience™ ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... incubated with goat anti-rat IgG Microbeads (Miltenyi Biotec, Auburn, CA) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: Highly concentrated (97%) parasites (strains 3D7 and Dd2) were isolated by magnetic separation at the schizont stage (LD columns, Miltenyi Biotec, UK) and re-suspended in complete medium with either beta thalassaemic or non-thalassaemic erythrocytes at 0.05% Hct ...
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bioRxiv - Cell Biology 2022Quote: ... and then with anti-Rabbit IgG MicroBeads (Miltenyi Biotec, #130-048-602). The post-nuclear fractions were loaded on magnetic columns (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl of anti-rabbit IgG microbeads (Miltenyi biotec, cat# 130-048-602) were added to the sample followed by a 20 min incubation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with anti-rabbit IgG micro-beads (130-048-602, Miltenyi biotec) diluted in MACS buffer for 15 mins at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... before cells were incubated in anti-mouse IgG 2a+b microbeads (Miltenyi Biotec) for 20 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Negative cell populations will be incubated with anti-Mouse IgG beads (Miltenyi Biotec, 130-048-402), and then MS coulumn (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: ... Control devices were functionalized with biotinylated normal (non-specific) mouse IgG antibodies ((Miltenyi Biotec, Bergisch Gladbach, Germany) at 2 μg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... CV38-142 IgG used in the SPR assay was produced in CHO cells and was kindly provided by Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Immunology 2024Quote: CAR+ T-cells were purified using a biotin-conjugated AffiniPure Goat Anti-Mouse IgG F(ab’)₂ fragment specific antibody and anti-biotin microbeads (Miltenyi Biotec). In the case of dual knock-in TET2-TRAC CAR T-cells ...
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bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...