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Citations for Miltenyi Biotec :
1 - 50 of 50 citations for Round Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was designed and prepared in 96-well glass bottom plates (Miltenyi Biotec) using a protocol for solid phase reverse transfection as previously described16,42 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... polyA+ RNA was purified using the 96-well MultiMACS mRNA isolation kit on the MultiMACS 96 separator (Miltenyi Biotec, Germany) from total RNA with on column DNaseI-treatment as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Immunology 2022Quote: ... The library was selected for 3 rounds with streptavidin beads (Miltenyi Biotec) loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec) ...
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These samples were each added to 200 µL of filtered M9 salt solution inside the wells of a 96 well plate and BFP+ and YFP+ cellular events were quantified by flow cytometry using a Macsquant10 (Miltenyi biotec). The competition plates were then sealed with Breathe-Easier Sealing Film (Diversified Biotech) ...
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bioRxiv - Immunology 2022Quote: ... A second round of mechanical dissociation was performed using the gentleMACS Dissociator (Miltenyi Biotec), and dissociated cells were rinsed with 1X PBS (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... The resulting suspensions underwent rounds of filtering through mesh strainers (70 µm, Miltenyi Biotec cat ...
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bioRxiv - Immunology 2021Quote: ... Multi-96 Columns (Miltenyi Biotec) were placed into a MultiMACS M96 Separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... dendritic cells were purified with two rounds of MACS magnetic separation using mouse CD11c microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8 T cells were isolated from the LNs of NY 8.3 NOD mice using two rounds of the naïve 8.3 CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... while antibody rounds were either optically bleached or signal was removed using the REAlease® Release Reagent (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec). TCR tetramers were created by adding TCR to tetrameric AlexaFluor647-conjugated streptavidin (produced in-house ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMCs by three sequential rounds of magnetic separation using CD3 Microbeads (Miltenyi Biotec, 130-050-101), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The samples were mounted on in a glass bottom petri dish (Miltenyi Biotec) with a drop of SlowFade Diamond antifade mountant (Thermofisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... naïve 8.3 CD8 T cells from the LNs of non-diabetic NY8.3 NOD mice were purified using two rounds of the naïve CD8 T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2023Quote: ... Then Myelin Removal Beads II (130-96-733, Miltenyi Biotec) were used to remove myelin debris from single-cell suspensions ...
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bioRxiv - Neuroscience 2022Quote: ... 8 well chamber slide dishes (Miltenyi Biotec) were coated over night with 1 mg/ml poly-L-lysine in borate buffer (boric acid ...
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bioRxiv - Neuroscience 2021Quote: ... and myelin removal beads (Myelin Removal Beads II, 130-96-733, Miltenyi Biotec) were used according to the manufacturer’s protocol to prepare the cells for incubation with microbead-coupled anti-CD11b mAb ...
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bioRxiv - Neuroscience 2021Quote: ... and then myelin removal beads (Myelin Removal Beads II, 130-96-733, Miltenyi Biotec) were used according to the manufacturer’s protocol to prepare the cells for incubation with microbeads-coupled anti CD11b mAb ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Immunology 2023Quote: ... 5 % CO2 after which migrated cells in the bottom chamber were collected and acquired on a MACSQuant Analyzer 10 Flow Cytometer using the MACSQuantify software (Miltenyi Biotec). The percentage of specific migration was calculated by dividing the number of cells migrated to the lower well by the total cell input (50 µl cell suspension directly measured on the MACSQuant Flow Cytometer).
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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bioRxiv - Immunology 2023Quote: ... and FcgR block (0.5 µl/well, FcR Blocking Reagent mouse, Miltenyi Biotec) for 15 min at RT in the dark ...
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bioRxiv - Immunology 2024Quote: ... wells were stained in cold MACS buffer (Miltenyi Biotec, Cat. No.130-091-221) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... with 4000 cells/well in serum free medium (SFM) containing 10 ng/ml BMP4 (Miltenyi Biotec), 10 ng/ml bFGF (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... Sigma-Aldrich)-coated tissue culture treated 24 well plates and treated with IFN-γ (1ng/ml, Miltenyi Biotec) and TNFα (1ng/ml ...
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bioRxiv - Immunology 2022Quote: ... 100,000 cells per well were activated with anti-human TransAct (1:100, Miltenyi Biotec 130-111-160) in T cell medium (10 % FCS ...
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bioRxiv - Immunology 2021Quote: ... or overnight with 1 μg/peptide per well of spike associated (Cat: 130-127-951, Miltenyi Biotec Peptivator) peptides at 37°C ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from respective wells using NK cell isolation kit (Miltenyi biotec; Cat: 130-092-657) following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent ...
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bioRxiv - Immunology 2023Quote: ... a 24-well plate was pre-coated with anti-CD3 (OKT3) and anti-CD28 antibodies (15E8) (Miltenyi Biotec) at 1μg/mL on day 0 ...
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bioRxiv - Bioengineering 2023Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
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bioRxiv - Immunology 2021Quote: ... The right lungs as well as the right hemispheres of the brains were homogenized in 1 ml PBS using a gentleMACS Octo Dissociator (Miltenyi Biotec) and viral RNA was isolated from 140 µl cleared homogenate or BAL fluid using QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... The numbers of cells that migrated to the lower well after 1 hr incubation were counted using a MACSQuant flow cytometer (Miltenyi Biotec). The percent migration was calculated by the numbers of neutrophils that migrated into the bottom chamber divided by the total number of neutrophils in the starting cell suspension and multiplying the results by 100 ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were maintained in a feeder-free format on Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec) with daily feeding ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells from each well was seeded into three or four Matrigel-coated 12-well culture plates and fibroblast growth media was replaced with StemMACS iPS-Brew XF stem cell culture media (Miltenyi Biotec) and changed every day ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Microbiology 2022Quote: ... stained samples were diluted 10-fold in 1x PBS and cell concentrations as well as green fluorescence intensities were recorded on a MACSQuant Analyzer 10 (Miltenyi Biotec, Germany).
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bioRxiv - Immunology 2022Quote: ... Then 1.5x 105 cells/cm2 in 24-well dishes were seeded and mouse receptor activator of nuclear factor kb ligand (RANKL, Miltenyi Biotec, Germany) was added at the final concentration of 35 ng/ml to initiate osteoclasts (OC ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...