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Citations for Miltenyi Biotec :
1 - 46 of 46 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Dissected and trimmed tumors were washed in pre-cooled RNase-free H2O and transferred into MACS Tissue storage solution (Miltenyi Biotec, 130-100-008). Samples were shipped on ice overnight to Novogene ...
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bioRxiv - Microbiology 2024Quote: ... The harvested lungs were homogenized in 4.5 ml of distilled water with the gentleMACS™ Tissue Dissociator (Miltenyi Biotec, Bergish Gladbach, Germany) and the homogenates were plated on Middlebrook 7H10–oleic acid ADC (7H10-OADC ...
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bioRxiv - Genomics 2024Quote: ... rings and free merozoites was passed through LD columns (Miltenyi Biotec). The flow-through ...
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bioRxiv - Microbiology 2023Quote: ... Conidia suspensions were prepared by rinsing the plates with sterile distilled water and passed through a 20-µm cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany) to eliminate residual mycelium ...
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bioRxiv - Immunology 2023Quote: ... in RPMI before resting overnight in serum-free TexMACS medium (Miltenyi Biotec) supplemented with 1% Penicillin/Streptomycin at 37°C in a 96-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs were cultured under feeder-free conditions in StemMACs iPS-Brew (Miltenyi Biotec) on vitronectin-coated 6-well plates with daily medium changes ...
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bioRxiv - Immunology 2021Quote: ... with 4000 cells/well in serum free medium (SFM) containing 10 ng/ml BMP4 (Miltenyi Biotec), 10 ng/ml bFGF (Peprotech) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were neutralized with Ca2+/Mg2+ free DPBS with 0.5% BSA (Miltenyi Biotec, 130-091-376) and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec ...
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bioRxiv - Genomics 2021Quote: ... ESC were adapted to 2i media (serum-free N2B27 medium supplemented with MEK inhibitor PD0325901 [0.4 μM, Miltenyi Biotec] ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed twice with serum free medium prior to FACS analyses (MACSQuant Analyzer, Miltenyi Biotec, Bergisch Gladbach, Germany). A forward- and side-scatter gate was set to exclude dead cells with forward-scatter (FSC ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were maintained in a feeder-free format on Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec) with daily feeding ...
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bioRxiv - Bioengineering 2024Quote: iPS11 wild type (WT) and transgenic cell lines were grown in feeder-free conditions such as hiPSC Brew XF medium (Miltenyi Biotec) in Geltrex (Life technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein complexes with TTL3-GFP and free GFP as a control were isolated using the μMACS and MultiMACS GFP Isolation Kits (Miltenyi Biotec, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2021Quote: ... The large airways were removed and approximately ~500mg of airway free tissue was collected in a gentleMACS C-tube (Miltenyi Biotec, Auburn, CA) and 5mL digest buffer was added and placed on a gentleMACS octo dissociator for programmed runs ...
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bioRxiv - Cancer Biology 2020Quote: ... MSC were grown from the fragments retained in the cell strainers in serum-free StemMACS MSC Expansion Media XF (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells counts were determined by flow cytometer Novocyte (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Cancer Biology 2024Quote: ... + 10 U/μL DNase I DNase I (Roches)) or MACS Human Tumor Dissociation kit (DS_130-095-929, Miltenyi Biotec, Germany). The incubation was terminated when the digestive fluid turned turbid and the tissue block disappeared ...
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bioRxiv - Immunology 2021Quote: ... with DNAse for 30 minutes then dissociated via gentleMACS Dissociator (Miltenyi Biotec) and lymphocytes purified on a 44/67% Percoll (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... and DNAse I (BioConcept) enzyme mix and the gentleMACS Octo Dissociator (Miltenyi Biotec). For the isolation of immune cells from the lung tissue ...
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bioRxiv - Microbiology 2021Quote: ... and DNase I (Spectrum) using gentleMACS C tubes according to the manufacturer’s recommendations (Miltenyi Biotec). Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend) ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... cells were washed once more in Milli-Q water containing 1X BSA (Miltenyi Biotec #130-091-376) and 1X Annexin V Binding Buffer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... in cTubes containing lung dissociation kit reagents (DNAse and collagenase ref no.130-095-927, Miltenyi Biotec). Lungs ...
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bioRxiv - Genetics 2024Quote: ... then washed with 3 mL washing buffer (PBS + 1% BSA + 0.2 U/μL RNase inhibitor) and filtered through 30 μm MARC SmartStrainer (Miltenyi Biotec) twice ...
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bioRxiv - Immunology 2024Quote: Frozen tissue pieces were quickly thawed in a water bath before transferring into a GentleMACS dissociation tube (Miltenyi Biotec) containing Advanced DMEM/F12 + 1:100 Glutamax (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... 0.01% BSA and 40 U/ml RNase inhibitor) and filter through 20 µm pre-separation filters (Miltenyi Biotec, 130-041-407) and were centrifuged with a swing bucket at 500xg ...
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bioRxiv - Immunology 2024Quote: ... Samples were digested (100µg/ml DNase and 0.4 mg/ml collagenase in 10% FBS/RPMI) using GentleMacs Dissociator (Miltenyi biotec), followed by filtering with 70μm strainer using 10% FBS/RPMI ...
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bioRxiv - Immunology 2020Quote: ... 20μg/mL DNase in 1x HBSS with MgCl2 and CaCl2) running the default Lung_01 protocol on a gentleMACS Dissociator instrument (Miltenyi Biotec). Tumor samples were then incubated at 37°C for 40min (rotating ...
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bioRxiv - Immunology 2024Quote: ... Grafts were flushed with saline and digested with collagenase A and DNase I in a gentleMACS tissue dissociator (Miltenyi Biotec). Cells were stained with cocktails of fluorochrome-conjugated monoclonal antibodies including AF647- conjugated anti-mouse CD57 (polyclonal ...
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bioRxiv - Molecular Biology 2024Quote: ... pooled honey bees (n=30) were dissociated with 15 mL of molecular grade water using gentleMACS™ M Tubes (Miltenyi Biotec, USA) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... 5ml of fresh ACME solution (15% methanol/10% glacial acetic acid/10% glycerol in Milli-Q water) were added to labelled polyps in gentleMACS™ C-tubes (Miltenyi Biotec). During the ACME incubation ...
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bioRxiv - Microbiology 2021Quote: ... were pooled and dissociation was done using a collagenase I and II and a DNase I enzyme mix (all from BioConcept) and the gentleMACS Octo Dissociator (Miltenyi Biotec). Following this mechanical and enzymatical dissociation ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue of single mice was digested with trypsin and DNase according to the guidelines of the Neural Tissue Dissociation Kit (T) (Miltenyi Biotec) using a Gentle MACS Dissociator (Miltenyi Biotec) ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... with 10 ml of sterile distilled water using a T-shaped inoculation spreader and spore suspensions were filtered through a 30-μm cell strainer (MACS, Miltenyi Biotec GmbH, Germany) to exclude mycelium ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of microdissected vmPFC grey matter was homogenized in 2 ml of cold homogenization buffer (1M sucrose + 1% BSA dissolved in DEPC-treated water) using the benchtop gentleMACS™ Dissociator (Miltenyi Biotec, Germany) with the rotating paddle set to the Lung 02.01 program ...
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bioRxiv - Neuroscience 2022Quote: ... At the end of the incubation the single cell suspension was washed with 500 μL wash buffer (0.02% BSA in 1X PBS with 80 U/mL Protector RNase Inhibitor) and passed through a 20 μM filter (MACS, Miltenyi Biotec cat#130-101-812) to a fresh 2 mL Protein LoBind Eppendorf ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Immunology 2024Quote: Mouse whole blood samples were processed as follows: 200 µL of blood was incubated with 2ml of 1x Red Blood Cell Lysis buffer diluted in water (Miltenyi Biotec #130-094-183) for 10min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were incubated in a water bath at 37°C for 35 min and processed using a MACS™ Dissociator (Miltenyi Biotec, 130-093-235). The homogenate was filtered through a 40 µm cell strainer ...
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bioRxiv - Genetics 2022Quote: ... 150 µl of 1M HEPES and 0.75 µl of 10 mg/ml DNAse I) and dissociated in gentleMACS Dissociator (Miltenyi Biotec Inc., cat. no. 130-093-235) using the Multi_E_01.01 program ...