Labshake search
Citations for Miltenyi Biotec :
1 - 30 of 30 citations for Arsenazo I Trisodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
-
bioRxiv - Cancer Biology 2024Quote: ... + 10 U/μL DNase I DNase I (Roches)) or MACS Human Tumor Dissociation kit (DS_130-095-929, Miltenyi Biotec, Germany). The incubation was terminated when the digestive fluid turned turbid and the tissue block disappeared ...
-
bioRxiv - Microbiology 2021Quote: ... were pooled and dissociation was done using a collagenase I and II and a DNase I enzyme mix (all from BioConcept) and the gentleMACS Octo Dissociator (Miltenyi Biotec). Following this mechanical and enzymatical dissociation ...
-
bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
-
bioRxiv - Immunology 2020Quote: ... cells were labelled with APC IFN-I (Miltenyi Biotec) at 4◻C 30 minutes.
-
bioRxiv - Immunology 2021Quote: ... the Pan T Cell Isolation Kit I for mouse (Miltenyi Biotec) was used two consecutive times to reach >98% T cell purity after red blood cell lysis ...
-
bioRxiv - Developmental Biology 2023Quote: Images were acquired with an ultramicroscope I (LaVision BioTec, Miltenyi Biotec) coupled to a 2x objective (Olympus ...
-
bioRxiv - Immunology 2021Quote: ... I-Ek VioBlue (REA510) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Cells were analyzed with a BD FACSAria II ...
-
bioRxiv - Immunology 2020Quote: ... and DNAse I (BioConcept) enzyme mix and the gentleMACS Octo Dissociator (Miltenyi Biotec). For the isolation of immune cells from the lung tissue ...
-
bioRxiv - Neuroscience 2023Quote: ... were sacrificed at E14.5 and brain cortices dissected under a stereomicroscope in ice-cold Hanks’ Balanced Salt Solution (HBSS) and enzymatically dissociated using a Neural Tissue Dissociation Kit (Miltenyi Biotec) following manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... 500U/ml Collagenase I) with a tissue-lyser (Gentle MACS, Miltenyi Biotec, Bergisch Gladbach, Germany) and digested enzymatically for 20-30 min at 37°C while continuously shaking in a water bath ...
-
bioRxiv - Microbiology 2021Quote: ... and DNase I (Spectrum) using gentleMACS C tubes according to the manufacturer’s recommendations (Miltenyi Biotec). Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend) ...
-
bioRxiv - Immunology 2023Quote: ... Naïve OT-I cells were isolated from lymph nodes using magnetic CD8-microbeads (Miltenyi Biotec) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... 500U/ml Collagenase I) with a tissue-lyser (Gentle MACS, Miltenyi Biotec, Bergisch Gladbach, Germany, Germany) and digested enzymatically for 20-30 min at 37°C while continuously shaking in a water bath ...
-
bioRxiv - Cancer Biology 2023Quote: ... Healthy-adjacent tissues were dissociated using Tissue Dissociation Kit I (Miltenyi Biotec, cat.no. 130-110-201). After dissociation ...
-
bioRxiv - Immunology 2024Quote: ... OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... OT-I T cells were purified from spleens with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec), activated and cultured as previously described (11).
-
bioRxiv - Biophysics 2022Quote: ... and REA control (I)-APC human for the control of unspecific binding (Miltenyi Biotec, Ref. 130-104- 615). At least 10000 events were gated according to the FSC/SSC dot plot with the exclusion of dead cells.
-
bioRxiv - Immunology 2024Quote: ZFP36L2CKO and WT OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... Grafts were flushed with saline and digested with collagenase A and DNase I in a gentleMACS tissue dissociator (Miltenyi Biotec). Cells were stained with cocktails of fluorochrome-conjugated monoclonal antibodies including AF647- conjugated anti-mouse CD57 (polyclonal ...
-
bioRxiv - Microbiology 2021Quote: ... cultures of the two parasite lines for comparison were (i) synchronized to 80% late schizonts using MACS purification columns (Miltenyi Biotec) (ii ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
-
bioRxiv - Immunology 2022Quote: ... 0.1 mg ml−1 DNAase I in RPMI-1640 medium and enriched by depleting CD45+ cell and TER119+ cells using MACS microbeads (Miltenyi Biotec). Cells suspensions were incubated with anti-CD45 (30-F11 ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were transferred to a C Tube containing the enzyme mix I (enzyme P and buffer Z from the Adult Brain Dissociation Kit #130-107-677, Miltenyi Biotec, mixed according to manufacturer’s instructions) ...
-
bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
-
bioRxiv - Immunology 2024Quote: ... and presentation of SIINFEKL peptide in the context of MHC Class I on the surface of MC-38 cells was determined by flow cytometry (H-2Kb/SIINFEKL, REA1002, Miltenyi Biotec). The MC-38 cell line was maintained as a pool ...
-
bioRxiv - Immunology 2021Quote: ... were isolated from the spleen of naive Rdh10f/f Cd4Cre Rag1-/- OT-I CD45.1+ CD45.2+ (Rdh10CKO) or Rdh10f/f Rag1-/- OT-I CD45.1+ (control) mice using the Pan T Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany). For the adoptive transfer of memory T cells ...
-
bioRxiv - Immunology 2020Quote: ... The supernatant was removed and the pellet resuspended and processed as directed by the Multi-tissue Dissociation Kit I (Miltenyi Biotec, 130-110-201). Brains were enzymatically digested into a single cell suspension by rotating at 37°C for approximately 25 minutes with trituration halfway through incubation ...
-
bioRxiv - Genetics 2022Quote: ... 150 µl of 1M HEPES and 0.75 µl of 10 mg/ml DNAse I) and dissociated in gentleMACS Dissociator (Miltenyi Biotec Inc., cat. no. 130-093-235) using the Multi_E_01.01 program ...