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Citations for Miltenyi Biotec :
1 - 43 of 43 citations for ALLYL 11 12 EPOXY STEARATE TECH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Human IL-11 (Miltenyi Biotec ref# 130-094-623) and human IL-1β (Enzo Life Sciences ref# ALX-522-056 ...
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bioRxiv - Cancer Biology 2020Quote: ... 12 LS columns (Miltenyi Biotec) were used per fetal liver sample ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... 12 ng/ml FGF-2 (Miltenyi Biotec) and 20 ng/ml Activin A (PeproTec ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 μg/mL Hoechst 33342 (Miltenyi Biotec, Germany) were used to stain the live cells ...
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bioRxiv - Bioengineering 2024Quote: Human peripheral blood was obtained through venipuncture under Georgia Tech Institutional Review Board (IRB)-approved protocols and isolated by negative selection according to manufacturer instructions (Miltenyi Biotec 130-104-434, 130-098-196). Trans-epithelial electrical resistance was measured as previously described with the EVOM2 and STX HTS for Corning 96 (World Precision Instruments)41 ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Cancer Biology 2024Quote: ... TYR or Mart-1/MLANA PepTivator peptide pools (15-mer peptide sequences with 11 amino acids overlap, Miltenyi Biotec) for 60 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Microbiology 2023Quote: ... IL-18 (1,5 ng/ml) and IL-12 (1,25 ng/ml) (Miltenyi Biotec). After 20 hours of culture at 37°C ...
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bioRxiv - Immunology 2024Quote: Cytokines quantification has been performed using the MACSPlex Cytokine 12 Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and tumor necrosis factor (TNF) were determined by MACSPlex Cytokine 12 Kit (Miltenyi Biotec). Supernatants of tumor cell lines were also used for the measurement of FGL-1 with the Human FGL1/Fibrinogen Like Protein 1 ELISA Kit (Assay Genie ...
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bioRxiv - Genetics 2024Quote: ... isolated from 8–12 weeks old mice with CD43 magnetic beads (MACS, Miltenyi Biotec), were cultured in RPMI medium (Gibco ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
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bioRxiv - Genomics 2024Quote: ... the cytotrophoblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin (APC)-conjugated anti-human leukocyte antigen (HLA)-ABC antibody (APC-anti-HLA-ABC, Miltenyi Biotec, #130-101-467, lot #5191002371). The Hofbauer/fibroblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin-conjugated anti-epidermal growth factor (EGFR ...
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bioRxiv - Genomics 2024Quote: ... The Hofbauer/fibroblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin-conjugated anti-epidermal growth factor (EGFR) antibody (APC-anti-EGFR, Miltenyi Biotec, #130-110-529, lot #5190107002). Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec ...
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bioRxiv - Genomics 2024Quote: ... The EGFR-Hofbauer/fibroblast fraction was washed and incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ phycoerythrin (PE)-conjugated anti-CD10 antibody (anti-CD10-PE, Miltenyi Biotec, #130-114-5-2, lot #5190109168). The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Mouse spleen (∼ 0.1g) from 8-12-week-old C57B6 mice was placed in C-Tube (Miltenyi Biotec). After mechanical disruption ...
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bioRxiv - Immunology 2020Quote: ... PBMCs or purified NK cells were stimulated for 16-18 hours with 10 ng/mL rhIL-12 (Miltenyi Biotec), 100 ng/mL rhIL-15 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... Hemispheres were cut into 8-12 small pieces and placed into gentleMACS C-tubes (Miltenyi Biotec, #130-093-237) with dissociation solution prepared as instructed by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissues of primary GBM tumors (n=12) from patients were collected into MACS Tissue Storage Solution (Miltenyi Biotec) by surgical excision and next ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Immunology 2021Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells (Miltenyi Biotec, Cat#130-104-454) from Bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis at indicated time points ...
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bioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Cell Biology 2020Quote: ... and stimulated for 18 h with IL-2 (20 ng/ml; National Cancer Institute Preclinical Repository) and/or IL-12 (10 ng/ml; Miltenyi Biotec) cytokines ...
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bioRxiv - Immunology 2020Quote: T cells were isolated from splenocytes of 8-12-week-old mice using a Pan T Cell Isolation Kit II (Miltenyi Biotec) with >90% purity ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2023Quote: ... or Kdm6aflox/flox LckCre (UTXTCD) 8- to 12-week-old female donor spleens using the CD4+ T cell Isolation Kit II (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells from each well was seeded into three or four Matrigel-coated 12-well culture plates and fibroblast growth media was replaced with StemMACS iPS-Brew XF stem cell culture media (Miltenyi Biotec) and changed every day ...
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bioRxiv - Cell Biology 2023Quote: ... Day 12 CD34+ hematopoietic cells were purified via magnetic-activated cell sorting (MACS) using human CD34 microbeads (#130-046-702, Miltenyi Biotec). As previously reported [48] ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Cancer Biology 2023Quote: Human AML and CB cells were seeded for 10-12 days in methylcellulose-based semi-solid medium (StemMACS HSC-CFU Media complete with EPO, Miltenyi Biotec, Germany) for assessment of the frequency of colony formation ...
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bioRxiv - Immunology 2024Quote: ... the absence of exogenous Th17- promoting cytokines (ii) with 10ng/ml of human recombinant polarizing cytokines (i.e.,TGF-β, IL-4, IL-12; Miltenyi biotec, Germany) or WNT10a ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Microbiology 2021Quote: ... supernatants were collected and stored at −80 °C until analysis for cytokine secretion using the human MACSplex cytokine 12-kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Murine neutrophils were isolated from bone marrow of 7–12-week-old mice using an Anti-Ly-6G MicroBeads kit (Miltenyi Biotec; Cat. No. 130-120-337) per the manufacturer’s instructions ...