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Citations for Viagen Biotech :
1 - 43 of 43 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... in Direct PCR Lysis Buffer (Viagen) at 55° C for 2h ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened by PCR using primers that flank the SUMO4 gene using genomic DNA purified with direct PCR buffer (Viagen). Clones that displayed reduced size of SUMO4 PCR product were sequenced by Sanger sequencing to confirm disruption of the SUMO4 locus ...
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bioRxiv - Genomics 2023Quote: ... Individual nematode was isolated and washed with 10% bleach for 10 seconds and transferred into with 200µl PCR tube containing lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated using Viagen Direct PCR (Viagen). The presence of oncogenic and silent mutations in exons and insertions or deletions in introns of BRAF ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping of Fgd4fl/+ mice was performed by PCR on DNA isolated from tail biopsies using Direct PCR lysis reagent (Viagen Biotech Inc., USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in direct PCR reagent (Viagen 301-C) supplemented with 1 μL proteinase-K (Viagen 501-PK) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were digested in Direct PCR mix (Viagen #102-T) with 1.5% Proteinase K (Quiagen #19133 ...
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bioRxiv - Molecular Biology 2019Quote: gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA for PCR was extracted using DirectPCR Lysis Reagent (Viagen Biotech) plus 10 µg/ml proteinase K (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated with direct PCR lysis reagent (Viagen Biotech) with Proteinase K (O/N 55 °C) ...
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bioRxiv - Cell Biology 2023Quote: ... were lysed in Direct PCR (Tail) lysis buffer (Viagen, Biotech, #102-T) supplemented with 40 µg/mL Proteinase K (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 20 mg of each tissue were dissolved in direct PCR buffer (VIAGEN) with proteinase K overnight at 65°C ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA of was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... After adding 200 μl of Direct PCR Tail (Lysis buffer) (Viagen, 102-T) and 4 μl of proteinase K (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... A fraction of the expanded population was lysed in Direct PCR Lysis reagent (Viagen) with proteinase K (Roche) ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102-T), 1x Taq buffer (Syd Labs #MB042-EUT) ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Mice were genotyped using PCR on cut toes lysed in Direct Tail lysis buffer (Viagen) with primers as indicated in Supplemental Table S2.
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102- T), 1× Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Genomics 2023Quote: ... PCR using genomic DNA extracted from embryonic sacs digested with DirectPCR Lysis Reagent (Viagen, 301-C) containing Proteinase K (final concentration 6 U/mL ...
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bioRxiv - Neuroscience 2023Quote: Genotyping was done as follows: Genomic DNA was extracted from tail biopsies using PCR reagent (Viagen) supplemented with Proteinase K (1 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells collected from 96-well plates using Direct PCR lysis reagent (Viagen) supplemented with 1 mg/mL (w/v ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA: single worm DNA extract with lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were generated using cell lysate for each cell line as template (DirectPCR lysis reagent, Viagen), and then purified by using QIAquick 96 PCR Purification Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Mice were routinely genotyped using genomic DNA extracted from tail biopsies using the Direct PCR Lysis tail reagent (Viagen) supplemented with 5 mg/ml proteinase K (Worthington) ...
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bioRxiv - Cell Biology 2022Quote: ... Mice were routinely genotyped using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5□mg/mL proteinase K (Worthington) ...
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bioRxiv - Developmental Biology 2020Quote: Ear clips or yolk sacs were lysed either overnight (ear clips) or for 2 hours (yolk sacs) at 55°C in 200 μl of Direct PCR tail lysis buffer (Viagen) supplemented with 200 μg/ml Proteinase K (20 mg/ml stock solution) ...
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bioRxiv - Genetics 2021Quote: Crude lysates for conventional PCR genotyping were prepared from ear biopsies or yolk sacs using DirectPCR Lysis Reagent (Viagen Biotech). Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix ...
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bioRxiv - Cell Biology 2022Quote: Mice were genotyped by NGS using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5 mg/mL proteinase K (Worthington) ...
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bioRxiv - Developmental Biology 2024Quote: ... Established colonies were manually picked and expanded for screening indel mutations using PCR amplification of a region spanning the targeted gRNA region (genomic DNA isolated using DirectPCR Lysis Reagent (Tail) (VIAGEN), primers ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotypes were determined by polymerase chain reaction (PCR) after O/N digestion using DirectPCR (Tail) Lysis buffer (Viagen, Los Angeles, CA) per manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and genomic DNA was extracted using Direct PCR Lysis reagent (Viagen, 201-Y; 25 µl/well) with Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was isolated by enzymatic digestion of tail clips using Direct-PCR Lysis Reagent (Viagen Biotech, Los Angeles, CA, USA). Genotypes were determined by performing PCR amplification of genomic DNA using allele-specific primers ...
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bioRxiv - Immunology 2023Quote: ... Mouse genotypes were assessed by PCR from genomic DNA isolated from ear clips via digestion in DirectPCR Digestion Buffer + Proteinase K (Viagen Biotech) using the following primer pairs ...
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bioRxiv - Cancer Biology 2024Quote: Mouse genotypes were determined by PCR analysis of DNA derived from earclips or embryonic tails using direct tail lysis buffer (Viagen Biotech) and Proteinase K ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Microbiology 2022Quote: Crude lysates of single worms were produced as follows: 10 μl lysis buffer (100X stock solution: 1000 μl Direct PCR Lysis Reagent (Cell) (Viagen, 301-C), 50 μl 1 M DTT (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... tissue samples were collected via tail clipping and DNA was extracted using Direct PCR Lysis Reagent (Cat no. 101 101 T, Viagen Biotech, USA), and 1.5 µL of the lysate from each sample was used for PCR ...
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bioRxiv - Physiology 2024Quote: ... Genotyping was performed via PCR from tail clip biopsies after proteinase K digestion in DirectPCR tail lysis buffer (Viagen Biotech 102-T). PCR of genomic DNA was performed using Apex Taq Master Mix and allele-specific primers as previously described73.
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bioRxiv - Genomics 2022Quote: DNA for genotyping was extracted from tail clips as follows: tail clips were incubated overnight at 55° C in a solution of 200uL digestion/lysis buffer (Viagen Direct PCR (tail), Los Angeles ...
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bioRxiv - Cell Biology 2020Quote: ... The mice genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotyping of mice was performed by PCR with specific primers provided by the vendors using lysed tail from each mouse [0.3 cm tail in 100 mL of PCR Direct (tail) solution (Viagen Inc., Los Angeles, CA) with 1 mg/mL proteinase K at 55°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Single colonies were transferred to 96-well plates to be expanded and genotyped by PCR after crude DNA extraction with the DirectPCR Lysis Reagent (Viagen Biotech #302-C). Clones with a correct insert were further verified by Sanger sequencing and Western blot.