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Citations for Viagen Biotech :
1 - 36 of 36 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... T cells were lysed using DirectPCR (cell) (Viagen Biotech, LA, USA) lysis buffer supplemented with proteinase K (10 µg/mL ...
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bioRxiv - Immunology 2022Quote: Lysis: Sorted cells were lysed in 40 µl DirectPCR Lysis Reagent (Cell) from Viagen Biotech with 0.4 mg Prot K ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspending the cells in a lysis solution (100 μL DirectPCR Lysis Reagent (Cell) (Viagen Biotech), 76 μL of water and 4 μL Proteinase K ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspending the cells in a lysis solution (100 μL DirectPCR Lysis Reagent (Cell) (Viagen Biotech), 76 μL of water and 4 μL Proteinase K ...
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bioRxiv - Cancer Biology 2022Quote: ... a snap frozen tumoroid cell pellet was lysed using DirectPCR Lysis Reagent for Cells (Viagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells pellets were lysed and gDNA extracted using DirectPCR lysis reagent CELL (Viagen, 301-C) and Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were generated using cell lysate for each cell line as template (DirectPCR lysis reagent, Viagen), and then purified by using QIAquick 96 PCR Purification Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech). 200ng DNA or the whole cell lysis from each cell population underwent 28-cycle PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... as single cells into 96-well plates to establish clonal populations and subsequently genotyped for deletions using DirectPCR Cell Lysis buffer (Viagen), and cells positive for deletions were expanded (Fig ...
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bioRxiv - Genomics 2023Quote: ... cells were lysed in DirectPCR lysis reagent (Viagen). Lyastes were subsequently assessed by running qPCR with primers annealing to the transposon sequences ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed using DirectPCR lysis reagent (Viagen Biotech) according to the manufacturer protocol and lysates were used in a screening PCR to identify edited cells ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in direct PCR reagent (Viagen 301-C) supplemented with 1 μL proteinase-K (Viagen 501-PK) ...
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bioRxiv - Developmental Biology 2023Quote: Tissue samples were lysed in DirectPCR lysis reagent (cell) (Viagen Biotech), and the LoxCode sequence amplified using a 3-round PCR ...
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bioRxiv - Systems Biology 2024Quote: ... cells were lysed with DirectPCR Lysis Reagent (Viagen Biotech 301-C). Genomic regions were PCR-amplified (Platinum SuperFi II PCR master mix ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA isolation was performed using DirectPCR Lysis Reagent (Cell) (Viagen Biotech), Proteinase K (EO0491 ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using 20 μL of DirectPCR Lysis Reagent Cell (Viagen) supplemented with 1:100 10 mg/mL Proteinase K (Bioline) ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using 20 μL of DirectPCR Lysis Reagent Cell (Viagen) and sgRNA target sites were amplified for TIDE analysis as described above (using primers SM8+9 (LAD2) ...
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bioRxiv - Immunology 2021Quote: ... 58α-β- T cell hybridomas were lysed using DirectPCR Tail Lysis Buffer (Viagen Biotech) with proteinase K (SIGMA ...
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bioRxiv - Immunology 2023Quote: ... 58α-β- T cell hybridomas were lysed using DirectPCR Tail Lysis Buffer (Viagen Biotech) with proteinase K (SIGMA ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from CD8+ T cells using the DirectPCR Lysis solution (Viagen Biotech) containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega) ...
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bioRxiv - Immunology 2019Quote: Sorted CD4+ and CD8+ T cells were lysed in DirectPCR Lysis Reagent (mouse tail) (Viagen Biotech) with 1mg/ml proteinase K (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Adherent cells were washed twice with Dulbecco’s PBS (-) (D-PBS (-)) and treated with DirectPCR Lysis Reagent (Viagen: 0.5X DirectPCR Lysis Reagent ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA of single-cell colonies was isolated using DirectPCR lysis solution (Viagen Biotech, Los Angeles, CA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted after lysing cells with 200μl of DirectPCR lysis reagent (Viagen biotech, 102-T) for 15min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells collected from 96-well plates using Direct PCR lysis reagent (Viagen) supplemented with 1 mg/mL (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by adding 180 μL of DirectPCR (Cell) Lysis Buffer (Catalog No. 301-C, Viagen Biotech, St. Louis, MO) and 20 μL of Proteinase K (Catalog No ...
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bioRxiv - Genetics 2021Quote: ... cells were washed with 1x Ca2+/Mg2+-free PBS and resuspended in 50 μl of DirectPCR Lysis Reagent (Viagen Biotech) containing 0.4 mg/ml Proteinase K (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Editing control wells were harvested and cells were lysed with 30 μl DirectPCR lysis buffer (Viagen cat. no. 301-C) supplemented with 1 mg/ml proteinase K (Bioline ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was removed prior to the addition of 20 μL DirectPCR Lysis Reagent Cell (Viagen, cat. no. 301-C) supplied with 1:100 10 mg/mL Proteinase K (Bioline ...
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and genomic DNA was extracted using Direct PCR Lysis reagent (Viagen, 201-Y; 25 µl/well) with Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Genomic DNA was extracted from virally transduced HUVEC cells by: collecting approximately 10,000 cells, washing in PBS (sigma-Aldrich, US) and then lysing with DirectPCR Lysis Reagent (Viagen Biotech, US) containing Proteinase K (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... followed by the addition of 180 μL of DirectPCR (Cell) Lysis Buffer (Catalog No. 301-C, Viagen Biotech, St. Louis, MO) and 20 μL of Proteinase K (Catalog No ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with 500 μL cold PBS and then lysed with 100 μL DirectPCR Lysis Reagent (Viagen Biotech, 102-T) with 1% Proteinase K (Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Crude extracts were prepared for each clone: cells from a full 96-plate well were lysed in 25 µL DirecPCR Lysis Reagent (302-C, Viagen Biotec) supplemented with 100 µg/mL proteinase K and incubated at 55°C ...
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bioRxiv - Microbiology 2022Quote: Crude lysates of single worms were produced as follows: 10 μl lysis buffer (100X stock solution: 1000 μl Direct PCR Lysis Reagent (Cell) (Viagen, 301-C), 50 μl 1 M DTT (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Knock-out efficiency at the polyclonal level and frameshift confirmation of the individual clones was performed by isolating the cell genomic DNA using the DirectPCR Lysis Reagent (Viagen Biotech, Los Angeles, USA) and treatment with 0.36 mg/mL of proteinase K ...