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Citations for Viagen Biotech :
1 - 41 of 41 citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells collected from 96-well plates using Direct PCR lysis reagent (Viagen) supplemented with 1 mg/mL (w/v ...
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bioRxiv - Immunology 2022Quote: ... in Direct PCR Lysis Buffer (Viagen) at 55° C for 2h ...
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bioRxiv - Molecular Biology 2019Quote: ... Mice were genotyped using Direct Tail lysis buffer (Viagen) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated using Viagen Direct PCR (Viagen). The presence of oncogenic and silent mutations in exons and insertions or deletions in introns of BRAF ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in direct PCR reagent (Viagen 301-C) supplemented with 1 μL proteinase-K (Viagen 501-PK) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were digested in Direct PCR mix (Viagen #102-T) with 1.5% Proteinase K (Quiagen #19133 ...
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bioRxiv - Molecular Biology 2019Quote: gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated with direct PCR lysis reagent (Viagen Biotech) with Proteinase K (O/N 55 °C) ...
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bioRxiv - Cell Biology 2023Quote: ... were lysed in Direct PCR (Tail) lysis buffer (Viagen, Biotech, #102-T) supplemented with 40 µg/mL Proteinase K (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 20 mg of each tissue were dissolved in direct PCR buffer (VIAGEN) with proteinase K overnight at 65°C ...
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bioRxiv - Microbiology 2023Quote: ... or DirectPCR™ Lysis Reagent (Direct buffer) (Viagen Biotech Inc., CA, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA of was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... After adding 200 μl of Direct PCR Tail (Lysis buffer) (Viagen, 102-T) and 4 μl of proteinase K (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... A fraction of the expanded population was lysed in Direct PCR Lysis reagent (Viagen) with proteinase K (Roche) ...
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bioRxiv - Genetics 2019Quote: ... Lysis buffer was prepared by diluting one part direct-lysis reagent (301-C, Viagen Biotech) in two parts destilled water (e.g ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Mice were genotyped using PCR on cut toes lysed in Direct Tail lysis buffer (Viagen) with primers as indicated in Supplemental Table S2.
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bioRxiv - Genomics 2023Quote: ... Purified DNA: single worm DNA extract with lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping was performed using genomic DNA prepared from ear punch biopsies with the Direct DNA (Tail) (Viagen Biotech, USA). The Kcnq2Thr274Met/+ animals were maintained and studied on the 129Sv genetic background ...
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bioRxiv - Immunology 2021Quote: ... Mice were routinely genotyped using genomic DNA extracted from tail biopsies using the Direct PCR Lysis tail reagent (Viagen) supplemented with 5 mg/ml proteinase K (Worthington) ...
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bioRxiv - Cell Biology 2022Quote: ... Mice were routinely genotyped using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5□mg/mL proteinase K (Worthington) ...
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bioRxiv - Immunology 2022Quote: ... DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech). 200ng DNA or the whole cell lysis from each cell population underwent 28-cycle PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: Ear clips or yolk sacs were lysed either overnight (ear clips) or for 2 hours (yolk sacs) at 55°C in 200 μl of Direct PCR tail lysis buffer (Viagen) supplemented with 200 μg/ml Proteinase K (20 mg/ml stock solution) ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened by PCR using primers that flank the SUMO4 gene using genomic DNA purified with direct PCR buffer (Viagen). Clones that displayed reduced size of SUMO4 PCR product were sequenced by Sanger sequencing to confirm disruption of the SUMO4 locus ...
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bioRxiv - Cell Biology 2022Quote: Mice were genotyped by NGS using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5 mg/mL proteinase K (Worthington) ...
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bioRxiv - Genomics 2023Quote: ... Individual nematode was isolated and washed with 10% bleach for 10 seconds and transferred into with 200µl PCR tube containing lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and genomic DNA was extracted using Direct PCR Lysis reagent (Viagen, 201-Y; 25 µl/well) with Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was isolated by enzymatic digestion of tail clips using Direct-PCR Lysis Reagent (Viagen Biotech, Los Angeles, CA, USA). Genotypes were determined by performing PCR amplification of genomic DNA using allele-specific primers ...
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bioRxiv - Cancer Biology 2024Quote: Mouse genotypes were determined by PCR analysis of DNA derived from earclips or embryonic tails using direct tail lysis buffer (Viagen Biotech) and Proteinase K ...
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bioRxiv - Microbiology 2022Quote: Crude lysates of single worms were produced as follows: 10 μl lysis buffer (100X stock solution: 1000 μl Direct PCR Lysis Reagent (Cell) (Viagen, 301-C), 50 μl 1 M DTT (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... tissue samples were collected via tail clipping and DNA was extracted using Direct PCR Lysis Reagent (Cat no. 101 101 T, Viagen Biotech, USA), and 1.5 µL of the lysate from each sample was used for PCR ...
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bioRxiv - Genomics 2022Quote: DNA for genotyping was extracted from tail clips as follows: tail clips were incubated overnight at 55° C in a solution of 200uL digestion/lysis buffer (Viagen Direct PCR (tail), Los Angeles ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping of Fgd4fl/+ mice was performed by PCR on DNA isolated from tail biopsies using Direct PCR lysis reagent (Viagen Biotech Inc., USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotyping of mice was performed by PCR with specific primers provided by the vendors using lysed tail from each mouse [0.3 cm tail in 100 mL of PCR Direct (tail) solution (Viagen Inc., Los Angeles, CA) with 1 mg/mL proteinase K at 55°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... the test plate was washed twice with PBS and DirectPCR lysis reagent (Viagen) mixed 1:1 (vol ...
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bioRxiv - Genetics 2023Quote: ... as single cells into 96-well plates to establish clonal populations and subsequently genotyped for deletions using DirectPCR Cell Lysis buffer (Viagen), and cells positive for deletions were expanded (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μl of Proteinase K (20 mg/ml; Viagen Biotech, Los Angeles, CA; Cat: 501-PK) were added to the biopsy sample ...
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bioRxiv - Molecular Biology 2024Quote: ... Crude extracts were prepared for each clone: cells from a full 96-plate well were lysed in 25 µL DirecPCR Lysis Reagent (302-C, Viagen Biotec) supplemented with 100 µg/mL proteinase K and incubated at 55°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Single colonies were transferred to 96-well plates to be expanded and genotyped by PCR after crude DNA extraction with the DirectPCR Lysis Reagent (Viagen Biotech #302-C). Clones with a correct insert were further verified by Sanger sequencing and Western blot.
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bioRxiv - Molecular Biology 2021Quote: ... DNA from two-cell stage mouse embryos was extracted by transferring the embryos one hour and six hours after injection into 5 μl lysis buffer (DirectPCR buffer, Viagen, supplemented with 2.5 μg/ml Proteinase K ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was isolated from a 5 mm tail biopsy using a rapid digestion method where 95 μl of lysis buffer reagent (Viagen Biotech, Los Angeles, CA; Cat: 102-T) and 5 μl of Proteinase K (20 mg/ml ...