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Citations for Becton, Dickinson and Company :
2051 - 2100 of 2359 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000-350,000 donor cells or the same volume of empty HBSS+2% FBS vehicle were intraperitoneally (IP) injected using a 30 G insulin syringe (BD Biosciences cat# 328431). Transplanted cells were allowed 2 weeks to engraft prior to SCI.
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bioRxiv - Neuroscience 2024Quote: ... Cell counting was performed in a hemocytometer and 2 million cells from each spleen were used for immunostaining and analysis in a flow cytometer (BD LSRFortessaTM X-20).
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bioRxiv - Immunology 2024Quote: ... 2×106 cells were stained as described above and sorted using a BD FACSAria II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). In the control group ...
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bioRxiv - Biochemistry 2024Quote: Wild type RPE1 cells or RanGAP1ΔNES clones 2 and 10 were grown on 10 cm plates to 40-70% confluence and pulsed with 10 µM BrdU (BD Pharmigen flow kit) for 45 minutes ...
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bioRxiv - Bioengineering 2024Quote: The following antibodies were used for cell staining/blocking prior to the secretion incubation (20-30 minutes at 4°C in 2% FBS/PBS): i) one-cell discovery campaigns – Fc-block (5 μg/ml; BD Biosciences cat# 553142) and anti-mouse TACI-APC (3 μg/ml ...
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bioRxiv - Genomics 2020Quote: ... TFA4 strain was cultivated in nutrient Zobell medium (4 g L−1 peptone and 1g L−1 yeast extract Becton, Dickinson and Company, Sparks ...
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bioRxiv - Microbiology 2021Quote: ... After 48h single colonies were picked and inoculated into 15ml falcon tubes containing 3ml nutrient broth (5g/L peptone BD difco, BD Bioscience; 3g/L yeast extract BD difco, BD Bioscience), and were grown overnight at 28°C shaken at 250rpm ...
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bioRxiv - Cell Biology 2020Quote: ... Acid citrate dextrose (ACD) tubes (Sol. B, Vacutainer®, BD, Switzerland); coverslips (18 mm diameter ...
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bioRxiv - Biophysics 2022Quote: ... and 0.5% (wt/vol) casamino acids (BD Bacto, cat. No. 223050)] ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC) complex (BD), 0.5% glycerol and 0.05% Tween 80 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with an oleic acid–albumin–dextrose–catalase mixture (BD Biosciences) and mycobactin J (Allied Monitor ...
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bioRxiv - Immunology 2022Quote: ... Blood samples were collected in ethylenediaminetetraacetic acid (EDTA) evacuated tubes (BD), and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Microbiology 2022Quote: ... Media supplements – casamino acids and yeast extract were purchased from BD Scientific ...
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bioRxiv - Microbiology 2023Quote: ... as follows: 0.5 % (w/v) Bacto Casamino Acids Technical (CAA; BD) was incubated for 5 h with 5 g/L chelex ...
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bioRxiv - Immunology 2024Quote: ... Fluorescent acid-fast staining using TB Fluorescent Stain Kit M (BD) was performed in combination with neutral-lipid staining dye Nile red (Sigma ...
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bioRxiv - Systems Biology 2024Quote: ... 0.05% Tween80 and 10% oleic acid-albumin-dextrose-catalase (OADC, BD). Prior to macrophage infections ...
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bioRxiv - Immunology 2024Quote: ... Blood samples were collected in ethylenediaminetetraacetic acid (EDTA) evacuated tubes (BD), and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Cancer Biology 2024Quote: Peripheral blood was collected in ethylenediaminetetraacetic acid (EDTA) (Beckton Dickinson (BD), 367525)-containing tubes and was processed within 1-2 h of blood draw ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in 200 μL of FCM buffer including 20 μL 7-amino-actinomycin D (7-AAD) viability staining solution (BD) and incubated for 10-30 minutes before acquisition ...
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bioRxiv - Biochemistry 2021Quote: ... 1 g/L yeast extract (BD Biosciences, San Jose, CA), 0.4 mM L-cysteine ...
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bioRxiv - Systems Biology 2021Quote: ... which contains 1.7g/L Yeast Nitrogen Base (YNB) (BD Difco) and 5g/L ammonium sulfate (EMD) ...
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bioRxiv - Microbiology 2021Quote: ... 24 g/L potato dextrose (BD Biosciences, San Jose, CA) and 2% granulated agar (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... This medium contains 37g/L Brain Heart Infusion (BD Biosciences) supplemented with 5 mg/L of hemin ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mL/L insulin-transferrin-selenium (ITS Premix; BD Biosciences), 25 μg/mL bovine pituitary extract (Gemini Bio-Products) ...
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bioRxiv - Immunology 2020Quote: ... made by preparing 1 L Brain Heart Infusion Broth (BD), and immediately before starting cultures adding 1 mL bovine hemin stock (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... ClearColi were each grown in 1 L LB broth (BD) with ampicillin (50 mg/ml ...
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bioRxiv - Developmental Biology 2021Quote: Mouse ESCs were pre-differentiated to an epiblast-like state as previously described [2] by seeding fibronectin-coated dishes (BD Biosciences cat. no. 356008) at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat ...
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bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS 2% FBS and analyzed by flow cytometry using a FACSCanto and Novocyte flow cytometer (BD Biosciences and Acea Biosciences). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2020Quote: 2 × 106 cells were stimulated for 4 h at 23°C with 40 ng ml−1 PMA and 1 μg ml−1 ionomycin in the presence of 2 μM Monensin (Golgi-Stop, BD Biosciences, Cat: 51-2092KZ). Cells were washed twice with FACS buffer and stained for extracellular markers ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...