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1 - 7 of 7 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019

• SARS-CoV-2 manipulates the SR-B1-mediated HDL uptake pathway for its entry

  Congwen Wei, et al., bioRxiv - Microbiology 2020

  Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
• The effector recognition by synthetic sensor NLR receptors requires the concerted action of multiple interfaces within and outside the integrated domain

  Xin Zhang, et al., bioRxiv - Plant Biology 2022

  Quote: ... the Kd (the dissociation constant) values between the effector and the RGA5 HMAs were measured using Monolith NT.115 (NanoTemper Technologies) with 30 % LED power and fitted with Nano Temper Analysis Software (Version 1.5.41) ...
• Mapping protein-metabolite interactions in E. coli by integrating chromatographic techniques and co-fractionation mass spectrometry

  Mateusz Wagner, et al., bioRxiv - Biochemistry 2024

  Quote: ... containing 6.25 μM of His-tagged PyrE labeled with RED dye (Nanotemper Technologies, USA), with an equal volume of the respective ligand solution ...
• The histidine kinase PdtaS is a cyclic di-GMP binding metabolic sensor that controls mycobacterial adaptation to nutrient deprivation

  Vignesh Narayan Hariharan, et al., bioRxiv - Microbiology 2019

  Quote: ... Equilibrated mixtures containing 5µM of PdtaS-EGFP and varying concentrations of c-di-GMP were loading onto hydrophobic capillaries (NanoTemper Technologies). The laser on and off times were set at 30s and 5s respectively ...
• Pyroptosis inhibiting nanobodies block Gasdermin D pore formation

  Anja Kopp, et al., bioRxiv - Biochemistry 2023

  Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
• Anti-cancer drug Tamoxifen interferes with Mycobacterium tuberculosis PhoPR mediated signaling and inhibits mycobacterial growth

  Abhishek Garg, et al., bioRxiv - Biochemistry 2023

  Quote: ... Serial dilutions of TAM were made in 16 different capillaries containing 50 µM PhoR-GFP (such that the fluorescence in the NanoTemper™ Monolith is in the range of 1200-1400 U) ...
• Identification of RACK1A as a component of the auxin-ethylene crosstalk regulating apical hook development in Arabidopsis thaliana

  Qian Ma, et al., bioRxiv - Plant Biology 2024

  Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...