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Citations for NanoTemper :
1 - 38 of 38 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and the thermal stability was monitored from 20°C to 90°C at a heating rate of 1.5°C/min using the PR.ThermControl software (NanoTemper).
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bioRxiv - Cell Biology 2021Quote: ... 10 μl of the protein samples at 10 μM were measured in triplicates with a temperature ramp set to 1 °C/min from 20 °C-80 °C using standard treated capillaries (NanoTemper Technologies (#PR-C002)) ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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bioRxiv - Biochemistry 2024Quote: ... coli was labeled using the Monolith Lys-Tag Labeling Kit RED-NHS second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Each sample was transferred into a capillary and measured from 35 °C to 95 °C using a Tycho N6T (Nanotemper). Data were analyzed in Prism (GraphPad Software).
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bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
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bioRxiv - Genetics 2024Quote: ... MST was performed at 37°C using premium capillaries (NanoTemper) at medium MST power with the LED excitation-power set to automatic (excitation ranged from 20-50%).
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bioRxiv - Genetics 2024Quote: ... MST was performed at 37°C using premium capillaries (NanoTemper) at 30% LED excitation and medium MST power ...
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bioRxiv - Cancer Biology 2019Quote: ... at 22°C temperature using a Monolith NT.115 (NanoTemper Technologies). An initial “Capillary Scan” was performed to scan for fluorescence across the capillary tray to determine the exact position of each capillary before the MST measurement was started ...
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bioRxiv - Biochemistry 2022Quote: ... with a 30 °C/min increase rate (NanoTemper® Technologies GmbH). The Inflection temperature (Ti ...
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bioRxiv - Microbiology 2021Quote: The experiments were performed at 20°C on a Monolith NT.115 (Nanotemper) with Premium Coated (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Measurements were performed at 22°C on a Monolith NT.115 instrument (NanoTemper Technologies) using the red channel ...
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bioRxiv - Molecular Biology 2022Quote: ... Thermophoresis measurements were collected at 25°C using a Monolith NT.115 (NanoTemper Technologies) set to medium LED power and 20% excitation power ...
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bioRxiv - Microbiology 2021Quote: ... MST/TRIC measurements were performed at 20°C using a Monolith NT.115 instrument (NanoTemper) at 25% LED power and 20% MST laser power ...
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bioRxiv - Biophysics 2020Quote: ... Measurements at 80°C required capillary sealing (Capillary Sealing Paste and Capillary Sealing Applicators; NanoTemper Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... thermal melting was performed from 20 to 90°C using a PrometheusNT.48 DSF (Nanotemper) using high sensitivity capillaries ...
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bioRxiv - Microbiology 2023Quote: ... Light scattering measurements were performed at 20 °C using Prometheus Panta (NanoTemper Technologies GmbH, Munich, Germany).
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bioRxiv - Physiology 2024Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Microbiology 2022Quote: MST experiments were carried out at 22 °C on Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany) as described in the review entitled Molecular interaction studies using microscale thermophoresis 37 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20% LED power in standard capillaries at 25°C on a Monolith NT.115 device (NanoTemper Technologies). The data was analyzed using the MO.affinity analysis software (V2.3 ...
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bioRxiv - Biochemistry 2024Quote: ... Measurements were performed at 25 °C and at medium MST power in Monolith Premium Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Experiments were run at 20% excitation and high MST power at 23.0°C on a Monolith NT.115Pico (NanoTemper). Each experimental compound was run in triplicate.
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Molecular Biology 2020Quote: ... Intrinsic protein fluorescence at 330 nm and 350 nm was monitored between 15 and 90 °C in the Prometheus NT.48 instrument (Nanotemper), and the Tm values calculated within the accompanying software by taking the turning point of the first derivative of the F350:F330 ratio as a function of temperature.
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bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... Equilibrated mixtures containing 5µM of PdtaS-EGFP and varying concentrations of c-di-GMP were loading onto hydrophobic capillaries (NanoTemper Technologies). The laser on and off times were set at 30s and 5s respectively ...
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bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Then the mixture was incubated overnight at 16 °C followed by centrifugation at 15,000 g for 15 min before loaded into the Premium Capillaries (NanoTemper Technologies). The thermophoresis analysis ...
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bioRxiv - Biochemistry 2024Quote: ... Measurements were performed in triplets at 25 °C and at medium MST power in Monolith NT.115 Capillaries (NanoTemper Technologies). The raw data were analyzed using the MO.Affinity Analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
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bioRxiv - Biochemistry 2021Quote: ... Measurements were conducted at 40% MST power and light-emitting diode (LED)/excitation power setting 20% in Premium Coated capillaries on the Monolith NT.115 at 25°C (Nanotemper Technologies). Each experiment was performed in at least three replicates ...
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bioRxiv - Cell Biology 2023Quote: ... Measurements were performed at 22 °C using medium MST power and 100% excitation power settings on the MO.control data collection software (NanoTemper Technologies GmbH). The data were analyzed and fit using the MO.Affinity Analysis software (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2021Quote: Microscale thermophoresis measurements were performed at 25°C using Monolith NT™ premium capillaries and the Monolith NT.115 device with a green filter (NanoTemper, Germany). Power of LED and IR-laser sources are indicated in figure legends ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...