Labshake search
Citations for NanoTemper :
1 - 23 of 23 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... The 16 capillary tubes (NanoTemper Technologies) were inserted into each sample tube to enable sample entry into the capillary ...
-
bioRxiv - Microbiology 2023Quote: ... loaded into Monolith™ capillary tubes (NanoTemper Technologies), and analyzed using the Monolith NT.115 MiscroScale Thermophoresis instrument with MO.Control software (NanoTemper Technologies) ...
-
bioRxiv - Molecular Biology 2021Quote: ... the samples were loaded into premium coated capillary tubes (Nanotemper) before undergoing MST in a Monolith™ NT.115 instrument ...
-
bioRxiv - Biochemistry 2022Quote: ... the samples were loaded into premium coated capillary tubes (Nanotemper) before undergoing MST in a Monolith™ NT.115 instrument ...
-
bioRxiv - Biochemistry 2024Quote: ... the samples were loaded into capillary tubes for Tycho NT.6 instrument (NanoTemper). Tryptophan fluorescence at emission wavelengths of 350 nm and 330 nm were measured as the sample temperature was ramped from 35 °C to 95 °C according to the preprogrammed instrument protocol ...
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: We transferred the samples to glass capillaries tubes (MO-K022, NanoTemper Technologies, Munich, Germany). We acquired MST traces ...
-
bioRxiv - Cell Biology 2019Quote: ... Tubes were shifted to RT and mixtures were transferred to 16 standard capillaries (Nanotemper, Germany) according to the manufacturer’s manual ...
-
bioRxiv - Biophysics 2020Quote: ... 40 nM of each labeled Plexin construct was mixed with a serial dilution of unlabeled GTPases or Plexin-B1 in 0.2 mL micro reaction tubes (NanoTemper) and then transferred to premium capillaries (NanoTemper) ...
-
bioRxiv - Biophysics 2022Quote: ... LLPS) were prepared in 1.5 ml Eppendorf tubes and loaded into 10 μl high sensitivity microcapillaries (NanoTemper Technologies, Germany) and subjected to DLS measurements ...
-
bioRxiv - Biochemistry 2021Quote: ... the mixtures were left to incubate for 10 min at room temperature and subsequently loaded in premium coated capillary tubes (NanoTemper). Measurements were performed with a Monolith NT.115 (NanoTemper ...
-
bioRxiv - Plant Biology 2021Quote: ... Each reaction mixture was incubated for 10 min in a PCR-tube followed by thermophoresis in standard MST-grade glass capillaries in a Monolith NT.115 instrument (Nanotemper Technologies).
-
bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...
-
bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
-
bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
-
bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The thermostability of MAPK14 was measured in the presence of different concentrations of MBZ in 50 mM HEPES buffer in the presence of 200 mM NaCl and 2% DMSO at pH 7.5 using a label-free fluorimetric analysis with a Prometheus NT.Plex instrument (NanoTemper Technologies), as described previously [52] ...
-
bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
-
bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
-
bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...