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Citations for NanoTemper :
1 - 49 of 49 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Biochemistry 2020Quote: ... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... PrgU-His was labeled with the RED 2nd generation His-tag labeling kit (NanoTemper Technologies) according to the manufacturer’s instructions with a final concentration in the assay of 50 nM (molar dye ...
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bioRxiv - Cell Biology 2023Quote: His-tagged MLCK proteins were labeled using a His-Tag Labeling Kit RED-tris-NTA (NanoTemper). Microscale thermophoresis was performed using a Monolith NT.115 (NanoTemper) ...
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bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ...
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bioRxiv - Neuroscience 2023Quote: ... was labelled via His-tag with Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH). The labelled complex was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Microbiology 2019Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... his-tagged TLNRD1 was diluted to 100 nM and coupled with His-tag NT-647 dye (RED-tris-NTA NanoTemper, München, Germany) at room temperature for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Recombinant METTL16 MTD (residues 1-291) protein was labelled via His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH, Germany). The labelled protein was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Microbiology 2020Quote: ... Monolith His-Tag Labeling kit (MO-L018) was from NanoTemper Technologies.
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bioRxiv - Biochemistry 2022Quote: ... The HIS-tag fluorophores RED-tris-NTA 2nd Gen (NanoTemper MO-L018) and Atto-647 (Sigma 02175) ...
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: ... using a His-tag Labeling Kit (NanoTemper Technologies, cat. no. MO-L018). For measurements using CBP30 ...
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bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
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bioRxiv - Biochemistry 2024Quote: ... containing 6.25 μM of His-tagged PyrE labeled with RED dye (Nanotemper Technologies, USA), with an equal volume of the respective ligand solution ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit Red-tris-NTA (NanoTemper Technologies) and diluted in MST buffer (NanoTemper Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies, catalog #: MO-L018) was used to label recombinant His-α1-ERD (MyBioSource ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: His6-CTxA1 was labeled using Monolith NT His-Tag Labeling Kit RED-tris-NTA (NanoTemper). CTxB labeled with Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Pathology 2021Quote: Spike RBD proteins were labeled using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies) following manufacturer protocol at 2:1 protein to dye ratio ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies, Germany). For Zn2+ titration ...
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bioRxiv - Physiology 2024Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit RED-Tris-NTA second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: His6-tagged caspases were labelled using the His-tag labelling Kit RED-tris-NTA 2nd generation (NanoTemper). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... as recommended by the Monolith His-Tag Labeling Kit RED-tris-NTA protocol (NanoTemper Technologies, MO-L008). The binding buffer used for reactions [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labeled using His-Tag Labeling Kit RED-Tris-NTA 2nd Generation (NanoTemper, Cat# MO-L018). The proteins (at fixed concentration ...
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bioRxiv - Biochemistry 2019Quote: ... C0-C7 was fluorescently labeled with a commercial His-tag labeling kit according to manufacturer instructions (Nanotemper technologies). His-labeled C0-C7 fluorescence was measured by a red LED at 60-90% excitation power (RED filter ...
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bioRxiv - Microbiology 2021Quote: ... TgPRP4K protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies). The labeled TgPRP4K protein was adjusted to 100 nM with a buffer containing 30 mM Hepes (PH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Biochemistry 2019Quote: ... was labelled with 100 μM Monolith NTTM His-Tag Labeling kit RED-tris-NTA (L008, NANOTEMPER, Munich, Germany) 30 min at room temperature (RT ...
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bioRxiv - Plant Biology 2022Quote: ... The N-terminal His-tag was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany). After 10 minutes incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Cell Biology 2024Quote: Purified protein was labeled using the His-Tag labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, MO-L018) following the manufacturer’s directions ...
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bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
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bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Biochemistry 2021Quote: 6xHis-eIF1 was labeled with a Cy5 fluorophore through non-covalent linkage to the His-tag moiety using the Monolith Protein Labeling Kit RED-tris-NTA 2nd generation (NanoTemper Technologies). 40S subunits (0.58 nM –1.2 μM ...
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018, NanoTemper Technologies, GmbH, Munich, Germany) per the manufacturer’s instructions with slight modifications (43) ...
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bioRxiv - Biochemistry 2023Quote: The BD5.1 protein was labelled with the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper Technologies, Catalog# MO-L018) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: The melting temperature (T m) of sE2 (1mg/ml) was calculated using the Prometheus NT.48 (Nanotemper, Munich, Germany) during heating in a linear thermal ramp (1°C ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... smegmatis β clamp (5μM) in the presence and absence of GRS (15μM) were measured in UV-capillaries using the Tycho NT6 (NanoTemper Technologies) where the protein unfolding is followed by detecting the fluorescence of intrinsic tryptophan and tyrosine residues at both emission wavelengths of 350 nm and 330 nm.