Labshake search
Citations for NanoTemper :
1 - 47 of 47 citations for KGF 2 FGF 10 Human 171a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
-
bioRxiv - Biochemistry 2021Quote: ... Ammonium sulfate suspension of the MDH was centrifuged at 23,000 × g for 1 min at 4 °C and the precipitate was resuspended in the labeling buffer to gain 90 μL of 10 μM MDH and mixed with 10 μL of 300 μM of dye (NanoTemper Technologies) prepared in dimethyl sulfoxide to create 100 μl of dye-protein solution ...
-
bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
-
bioRxiv - Biophysics 2022Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
-
bioRxiv - Biophysics 2021Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
-
bioRxiv - Biophysics 2023Quote: ... 10 µL samples were aspirated in capillaries (TY-C001, Nanotemper, Germany), and intrinsic fluorescence was measured at 350 nm and 330 nm while the temperature was ramped from 35 °C to 95 °C at a rate of 30 °C/min ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 10 ul was sampled in a Monolith.NT115 capillary (NanoTemper MO-K022) and measurements were performed at 25°C using standard parameters ...
-
DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... 10 μl were loaded onto the Monolith NT.115 Premium Capillaries (NanoTemper). The MST signal was recorded with NT.Control v2.1.31 software and data was fitted to a Kd model with MO.Affinity Analysis v2.3 software.
-
bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
bioRxiv - Biophysics 2021Quote: ... 10 mM NaCl and transferred to capillaries (Standard treated, NanoTemper Technologies GmbH, Germany), measurements were performed at 37 °C with a constant blue LED power of 20% and at MST infrared laser powers of 40 % to induce thermophoretic motion ...
-
bioRxiv - Biophysics 2021Quote: ... 10 μl samples were manually loaded into nanoDSF Grade Standard Capillaries (NanoTemper Technologies) and transferred to a Prometheus NT.48 nanoDSF device (NanoTemper Technologies) ...
-
bioRxiv - Biochemistry 2019Quote: MST was measured on 10 μl reactions using standard capillaries (MO-K022, NanoTemper Technologies) on a Monolith NT.115 (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
-
bioRxiv - Immunology 2022Quote: ... and ∼10 µl of each diluted sample (in duplicates) was loaded into NanoDSF capillaries (NanoTemper Technologies). The temperature was raised from 20°C to 95°C at 1°C/min rate ...
-
bioRxiv - Biochemistry 2020Quote: ... Approximately 10 μL of each diluted sample was loaded into nanoDSF grade high-sensitivity capillaries (NanoTemper) in triplicate and placed on the instrument’s capillary loading tray ...
-
bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
-
bioRxiv - Biophysics 2020Quote: ... pH 8.2) was mixed with 10 µl of 300 µM NT647-N-hydroxysuccinimide fluorophore (NanoTemper Technologies) and incubated in the dark for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...
-
bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
-
bioRxiv - Biophysics 2021Quote: ... 10 μl of 100 nM labeled NHR2 in MST-buffer (included with the capillary set from NanoTemper) was mixed with 10 μl unlabeled NHR2 with concentrations ranging from 10.3 nM to 337.5 μM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The thermostability of MAPK14 was measured in the presence of different concentrations of MBZ in 50 mM HEPES buffer in the presence of 200 mM NaCl and 2% DMSO at pH 7.5 using a label-free fluorimetric analysis with a Prometheus NT.Plex instrument (NanoTemper Technologies), as described previously [52] ...
-
bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... Thermophoresis data were analyzed when fluorescence signals lies within the 10% acceptance range using MO Affinity Analysis software V2.1.2 (Nanotemper). Data were plotted using GraphPad Prism 4.0 software and fitted with nonlinear equation.
-
bioRxiv - Molecular Biology 2023Quote: ... The mixtures were incubated at room temperature for 10 min and transferred to 16 standard capillaries (Nanotemper, Germany). Data were acquired using medium MST power ...
-
bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10-µL of undiluted rAAV of each lot was loaded into a high sensitivity capillary (Nanotemper Cat#PR-C006) and run on a Nanotemper Prometheus NT.48 nanoDSF instrument ...
-
bioRxiv - Biochemistry 2019Quote: ... 10 μL of the different dilutions of samples were filled into hydrophilic treated capillaries (premium coated capillaries, NanoTemper Technologies) and measured after a 10 min equilibration at room temperature ...
-
bioRxiv - Biophysics 2022Quote: ... LLPS) were prepared in 1.5 ml Eppendorf tubes and loaded into 10 μl high sensitivity microcapillaries (NanoTemper Technologies, Germany) and subjected to DLS measurements ...
-
bioRxiv - Molecular Biology 2023Quote: ... a volume of 10 μl was then placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
-
bioRxiv - Biochemistry 2023Quote: ... Approximately 10 µL of each sample was loaded into Prometheus NT.48 Series nanoDSF high sensitivity capillaries (NanoTemper, Germany) and melting scans were performed using the Pr.ThermControl software (NanoTemper ...
-
bioRxiv - Bioengineering 2023Quote: ... The samples (10 µL at 0.2 mg/mL in HBS) were loaded into standard treated NT.115 capillaries (NanoTemper), and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper ...
-
bioRxiv - Cancer Biology 2023Quote: ... Aliquots (10 μl) of purified mTORC1_WT or mTORC1_EWEDdup variant were loaded into the Prometheus capillaries (Cat. PR-C002, NanoTemper Technologies), and the fluorescence intensity at 330 nm and 350 nm was recorded as a function of temperature from 10 °C to 90 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
-
bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
-
bioRxiv - Biochemistry 2022Quote: ... were done in the commercial MST buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2; Nanotemper Technologies) supplied with 0.05% Tween-20 ...
-
bioRxiv - Biochemistry 2021Quote: ... the mixtures were left to incubate for 10 min at room temperature and subsequently loaded in premium coated capillary tubes (NanoTemper). Measurements were performed with a Monolith NT.115 (NanoTemper ...
-
bioRxiv - Molecular Biology 2023Quote: ... pH6) in a volume of 10 μl and placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
-
bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
-
bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... The samples were loaded into the MonoLith NT.115 standard treated capillaries and incubated for 10 min before analysis using the Monolith NT.115 instrument (NanoTemper Technologies) at 21°C using medium laser power and 40 % LED power ...
-
bioRxiv - Cell Biology 2021Quote: ... proteins at 200 nM were incubated with varying concentrations of CaCl2 and ZnSO4 in a 10 µl mixture and loaded into NT.115 standard coated capillaries (NanoTemper Technologies). The buffers (25 mM HEPES ...
-
bioRxiv - Plant Biology 2022Quote: ... The complex was allowed to establish for 10 min at room temperature before the samples were loaded into the capillaries (MO-K025, NanoTemper Technologies). Binding was detected by a Monolith NT.115 instrument (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2022Quote: ... 13000 rpm), filled into Prometheus high-sensitivity capillaries (10 μL each, n=3) and transferred to a Prometheus Panta instrument (NanoTemper Technologies). DLS analysis of BbHtrA S/A rh and PDI were determined using Prometheus Panta’s size analysis function.
-
bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...