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Citations for NanoTemper :
1 - 48 of 48 citations for Cy7 Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... fluorescent dye (Cat no. L008, NanoTemper Technologies) and applied at the final concentration of 50 nM in His-SPN SEC buffer having 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: ... and labeled with RED-NHS dye (NanoTemper). PTX was prepared in 25 mM HEPES (pH 7.4) ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... using a RED-NHS 2ndgeneration labeling dye (NanoTemper). NHS ester groups covalently crosslink lysine residues ...
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bioRxiv - Plant Biology 2023Quote: The fluorescent dye NT-647 (MO-L001, NanoTemper Technologies) was used to label effector proteins GST or BSA ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Purified MEMO1 was labeled with Red-NHS dye (NanoTemper Technologies GmbH) and diluted in 10 mM MES ...
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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bioRxiv - Plant Biology 2022Quote: ... The RED-NHS 2nd generation amine reactive dye (MO-L011, NanoTemper Technologies) was used to label the purified SIRK1-ECD (2 µM ...
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bioRxiv - Microbiology 2019Quote: ... whilst LpoA was labelled on cysteines with NT647 RED-Maleimide reactive dye (Nanotemper) according to manufacturer’s instructions (Nanotemper ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Biophysics 2020Quote: ... Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies) in PBS-T (10 mM phosphate ...
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bioRxiv - Molecular Biology 2021Quote: Jagged1C2-EGF3,HA in SEC buffer was labelled with NT-547 dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... were labeled with the amine-reactive dye NT-647 Red-NHS (NanoTemper, Munich, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... containing 6.25 μM of His-tagged PyrE labeled with RED dye (Nanotemper Technologies, USA), with an equal volume of the respective ligand solution ...
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bioRxiv - Biophysics 2022Quote: ... was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... purified PurTCp was labeled with RED fluorescent dye NT-647 (RED-tris-NTA; NanoTemper Technologies) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper). A 1:1 serial dilution of unlabelled zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Cancer Biology 2019Quote: KDM4B was labeled with a cysteine reactive fluorescent dye (Protein Labeling Kit RED-MALEIMIDE, Nanotemper GmbH). A serial dilution of titrant was prepared in MST buffer containing 10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies, Germany). For Zn2+ titration ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by labelling with 30uM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at RT for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Purified DVD-actin was labelled using Monolith NT protein labelling kit RED-NHS (Amine Reactive) dye (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein (200 nM) was fluorescently labeled using a RED-tris-NTA 2nd generation dye (NanoTemper Technologies GmbH) and a 1:1 dye/protein molar ratio in the reaction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... followed by labeling with 30 μM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at Room Temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: ... followed by labeling with cysteine-reactive dye (30 mM; Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper) and incubated for 30 min at room temperature (RT ...
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bioRxiv - Molecular Biology 2022Quote: SiaQM in amphipol was labelled at a ratio of 0.8 dye / 1 protein following the protocol of the RED-NHS 2nd generation labelling kit (NanoTemper). Labelled SiaQM was held constant at 20 nM and SiaP was titrated at 16 concentrations starting at 2,900 μM and serially diluting 2-fold to 90 nM ...
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bioRxiv - Biochemistry 2021Quote: ... The purified PDZ domains were covalently labelled using a fluorescent dye reactive on amine following the manufacture’s protocol (Protein Labelling Kit RED-NHS, Nanotemper). A serial dilution of the WT and the P71L mutant of the E protein PBM peptide was prepared in the buffer containing PBS tween 20 0.05% ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD P291-Q296;AAG was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper), and the procedure was carried out as above with zRETECD P291-Q296;AAG -NHS-RED (50 nM ...
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bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... were fluorescently labelled following the manufacturer protocol for free amine coupling of the dye (RED-NHS 2nd Generation (amine reactive)) (NanoTemper) to lysine residues ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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bioRxiv - Molecular Biology 2023Quote: ... 650 nm fluorescent dye using the Monolith NT RED-NHS lysine labeling kit according to manufacturer’s protocol (NanoTemper, Munich, Germany). RED labeled untagged sP36 at 100 nM and His6-GlnK1 in 16 different concentrations ranging from 7.5 µM to 0.23 nM or 10 nM of sP36-RED and AmtB1-His6 in 16 dilutions ranging from 12.6 µM to 0.38 nM (all concentrations based on monomeric molecular mass ...
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bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
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bioRxiv - Biophysics 2022Quote: His-tagged NCTD was fluorescently labelled with NT-647 amine reactive dye from the Monolith NT Protein Labelling kit RED-NHS (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeling was performed by incubating PlaF with 15 μL dye (440 μM stock solution) for 2.5 h using the NHS labeling Kit (NanoTemper, Munich, Germany). PlaF was then transferred into a purification buffer using PD-10 columns ...
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bioRxiv - Biochemistry 2021Quote: ... Ammonium sulfate suspension of the MDH was centrifuged at 23,000 × g for 1 min at 4 °C and the precipitate was resuspended in the labeling buffer to gain 90 μL of 10 μM MDH and mixed with 10 μL of 300 μM of dye (NanoTemper Technologies) prepared in dimethyl sulfoxide to create 100 μl of dye-protein solution ...
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
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bioRxiv - Cancer Biology 2024Quote: ... 90 µl of 200 nM protein mixed 1:1 with 100 nM RED-tris-NTA 2nd generation dye (MO-L018, Nanotemper Technologies) and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... his-tagged TLNRD1 was diluted to 100 nM and coupled with His-tag NT-647 dye (RED-tris-NTA NanoTemper, München, Germany) at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
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bioRxiv - Biophysics 2023Quote: His-tagged NSP16 was diluted to 400 nM in nsp16 buffer D and mixed with an equal volume of 100 nM RED-tris-NTA 2nd Generation labeling dye (NanoTemper Technologies GmbH, München, Germany). The mixture was incubated on ice for 1 h and centrifuged at 15,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...