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1 - 8 of 8 citations for ATP citrate lyase Rabbit Recombinant mAb since 2019

• Functional and structural insights into the multi-step activation and catalytic mechanism of bacterial ExoY nucleotidyl cyclase toxins bound to actin-profilin

  Magda Teixeira-Nunes, et al., bioRxiv - Biophysics 2023

  Quote: ... 0.2 mM ATP) with 0.05 % Tween-20 on a Monolith NT.115 Microscale Thermophoresis (MST) device using standard treated capillaries (NanoTemper Technologies). Actin (final labelled-actin concentrations adjusted to 60 nM ...
• Quantitative proteomics identifies the universally conserved ATPase Ola1p as a positive regulator of heat shock response in Saccharomyces cerevisiae

  Stefan Dannenmaier, et al., bioRxiv - Cell Biology 2021

  Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
• Myosin-X and talin modulate integrin activity at filopodia tips

  Mitro Miihkinen, et al., bioRxiv - Cell Biology 2021

  Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ...
• Characterization of prohibitins as novel target to block asexual and sexual stage growth of Plasmodium falciparum

  Monika Saini, et al., bioRxiv - Molecular Biology 2022

  Quote: ... we evaluated the interaction of recombinant PfPHB1 and PfPHB2 proteins using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). Thermophoretic movement of a fluorescently labelled protein is measured by monitoring the fluorescence distribution ...
• Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia

  Sumiko Takao, et al., bioRxiv - Cancer Biology 2021

  Quote: Binding of purified recombinant KIX with fluorescein isothiocyanate (FITC)-conjugated peptides was measured by microscale thermophoresis using the Monolith NT.115 (NanoTemper Technologies). Assays were conducted in 50 mM sodium phosphate ...
• SARS-CoV-2 variants show temperature-dependent enhanced polymerase activity in the upper respiratory tract and high transmissibility

  Se-Mi Kim, et al., bioRxiv - Microbiology 2022

  Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
• Artemisinin-resistant Plasmodium falciparum Kelch13 mutant proteins display reduced heme-binding affinity and decreased artemisinin activation

  Abdur Rahman, et al., bioRxiv - Biochemistry 2024

  Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
• Small-molecule inhibitors of the RNA m⁶A demethylase FTO potently support the survival of dopamine neurons

  Simona Selberg, et al., bioRxiv - Neuroscience 2021

  Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...