Labshake search
Citations for NanoTemper :
1 - 24 of 24 citations for 8 4 Chlorophenylthio guanosine 3' 5' cyclic Monophosphate triethylammonium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...
-
bioRxiv - Immunology 2024Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
-
bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 µL reactions were incubated at room temperature for 5 min and 5 µL of the mixture was loaded into NT.115 capillaries (NanoTemper). MST was performed in Monolith NT.115 (NanoTemper ...
-
bioRxiv - Microbiology 2022Quote: ... to 0.1 µM purified ClpC1NTD-FITC was assayed in CPD supplemented with 0.05% Tween 20 and 8 mg/ml BSA by microscale thermophoresis using a Monolith NT.115 (NanoTemper) (Jerabek-Willemsen et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... centrifuged for 5 min and loaded to standard capillaries (Nanotemper, #MO-K022). To investigate competitive effects of different ligands ...
-
bioRxiv - Microbiology 2020Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
-
bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
-
bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
-
bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
-
bioRxiv - Biochemistry 2022Quote: ... 13000 rpm), filled into Prometheus high-sensitivity capillaries (10 μL each, n=3) and transferred to a Prometheus Panta instrument (NanoTemper Technologies). DLS analysis of BbHtrA S/A rh and PDI were determined using Prometheus Panta’s size analysis function.
-
bioRxiv - Microbiology 2022Quote: ... The labeled protein was 1:1 incubated with a series dilution of ABT199 (4 uM to 0.122 nM) to perform the microscale thermophoresis experiments in Monolith NT.115 (NanoTemper) with the setting of 40% MST power and 40% excitation power at 25 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
-
bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
-
bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
-
bioRxiv - Biochemistry 2020Quote: ... The concentration of EMC2•OG-488 was fixed at 50 nM and a dilution series of EMC8 or EMC9 ranging from 4 nM to 150 µM was used in the hydrophilic treated capillaries (NanoTemper Technologies). A Monolith NT.115 instrument (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2022Quote: ... Measurements were done in triplicate at multiple concentrations (50 µM, 5 µM, and 0.5 µM) in Tycho standard capillaries (Nanotemper Technologies). Data analysis was performed via MO Affinity Analysis Software v2.1.3 (Nanotemper Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... around 5 μl of the samples were loaded in MonolithTM Capillaries and MST analysis was carried out on the Instrument (Nanotemper).
-
bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
-
bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
-
bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... selected linear/circular aptamers were equally diluted with 5’ Cy5 labeled complementary strand to reach the optimum concentrations of 5 nM where satisfying fluorescence intensity was obtained for KD determination using Monolith device (NanoTemper, Germany). Affinity of the selected aptamers was evaluated in 16 test tubes where spike/RBD proteins were diluted by double from the initial concentration 1 mM and then 5 nM of aptamer/Cy5 labeled complementary strand complex were added to each protein dilution in final volume of 20 μl ...
-
bioRxiv - Biochemistry 2023Quote: ... MnCl2 and DTT were added just before the experiment) and incubated at RT for 5 min before samples were loaded with premium capillaries (NanoTemper Technologies, cat. no. MO-K025). Data were recorded sequentially at 40% ...