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Citations for NanoTemper :
1 - 49 of 49 citations for 7 S 17 S dihydroxy 8 E 10 Z 13 Z 15 E 19 Z Docosapentaenoic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... “hot region” from 0.5 t 1.5 s) using the MO.AffinityAnalysis V2.3 software (NanoTemper Technologies). Curve fitting for data presentation was performed by GraphPad Prism Version 6.07 (‘one site – total binding’).
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bioRxiv - Biophysics 2022Quote: ... Data of three independent replicates were analysed using the signal from an MST-on time at 1.5-2.5 s (MO.Affinity Analysis software version 2.3, NanoTemper Technologies).
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bioRxiv - Immunology 2023Quote: ... Obtained MST traces were analyzed at an MST-on time of 1.5 s using the MO.Affinity Analysis version 2.2.4 (NanoTemper Technologies) for each of the three potential interaction pairs ...
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bioRxiv - Molecular Biology 2023Quote: MST experiments to measure the binding affinities between TadAs and the 19-nt DNA substrate were performed on a Monolith NT.115 system (NanoTemper Technologies, Germany) using the nano BLUE detector ...
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bioRxiv - Biochemistry 2021Quote: ... Samples of 15 μL were placed in high sensitivity quarts capillaries (Nanotemper) and the temperature was ramped with 1 °C/min from 20 °C to 95 °C ...
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bioRxiv - Microbiology 2022Quote: ... to 0.1 µM purified ClpC1NTD-FITC was assayed in CPD supplemented with 0.05% Tween 20 and 8 mg/ml BSA by microscale thermophoresis using a Monolith NT.115 (NanoTemper) (Jerabek-Willemsen et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Intrinsic protein fluorescence at 330 nm and 350 nm was monitored between 15 and 90 °C in the Prometheus NT.48 instrument (Nanotemper), and the Tm values calculated within the accompanying software by taking the turning point of the first derivative of the F350:F330 ratio as a function of temperature.
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bioRxiv - Biochemistry 2022Quote: ... Then the mixture was incubated overnight at 16 °C followed by centrifugation at 15,000 g for 15 min before loaded into the Premium Capillaries (NanoTemper Technologies). The thermophoresis analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeling was performed by incubating PlaF with 15 μL dye (440 μM stock solution) for 2.5 h using the NHS labeling Kit (NanoTemper, Munich, Germany). PlaF was then transferred into a purification buffer using PD-10 columns ...
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bioRxiv - Biophysics 2022Quote: Reactions were incubated for 15 minutes at room temperature in the dark and loaded into Monolith NT.115 Capillaries (NanoTemper Technologies GmbH). Microscale thermophoresis measurements were performed using a Monolith NT.115 (NanoTemper Technologies GmbH ...
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bioRxiv - Microbiology 2019Quote: ... The mixtures were incubated for 15 min and added into glass capillaries and loaded into a Monolith NT.115 instrument (NanoTemper Technologies, Germany), the Kd values were determined using NanoTemper Analysis software (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... were incubated with 0 mM to 50 mM GMP/GDP-mannose at room temperature for 15 minutes before measuring the Tm using a Tycho NT.6 instrument (NanoTemper Technologies, Germany). All experiments were done in two biological repeats and three technical repeats ...
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bioRxiv - Biochemistry 2021Quote: ... Ammonium sulfate suspension of the MDH was centrifuged at 23,000 × g for 1 min at 4 °C and the precipitate was resuspended in the labeling buffer to gain 90 μL of 10 μM MDH and mixed with 10 μL of 300 μM of dye (NanoTemper Technologies) prepared in dimethyl sulfoxide to create 100 μl of dye-protein solution ...
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bioRxiv - Biophysics 2022Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
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bioRxiv - Biophysics 2021Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
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bioRxiv - Biochemistry 2021Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...
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bioRxiv - Biophysics 2023Quote: ... 10 µL samples were aspirated in capillaries (TY-C001, Nanotemper, Germany), and intrinsic fluorescence was measured at 350 nm and 330 nm while the temperature was ramped from 35 °C to 95 °C at a rate of 30 °C/min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10 ul was sampled in a Monolith.NT115 capillary (NanoTemper MO-K022) and measurements were performed at 25°C using standard parameters ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... 10 μl were loaded onto the Monolith NT.115 Premium Capillaries (NanoTemper). The MST signal was recorded with NT.Control v2.1.31 software and data was fitted to a Kd model with MO.Affinity Analysis v2.3 software.
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bioRxiv - Biophysics 2021Quote: ... 10 mM NaCl and transferred to capillaries (Standard treated, NanoTemper Technologies GmbH, Germany), measurements were performed at 37 °C with a constant blue LED power of 20% and at MST infrared laser powers of 40 % to induce thermophoretic motion ...
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bioRxiv - Biophysics 2021Quote: ... 10 μl samples were manually loaded into nanoDSF Grade Standard Capillaries (NanoTemper Technologies) and transferred to a Prometheus NT.48 nanoDSF device (NanoTemper Technologies) ...
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bioRxiv - Biochemistry 2019Quote: MST was measured on 10 μl reactions using standard capillaries (MO-K022, NanoTemper Technologies) on a Monolith NT.115 (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
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bioRxiv - Immunology 2022Quote: ... and ∼10 µl of each diluted sample (in duplicates) was loaded into NanoDSF capillaries (NanoTemper Technologies). The temperature was raised from 20°C to 95°C at 1°C/min rate ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 10 μL of each diluted sample was loaded into nanoDSF grade high-sensitivity capillaries (NanoTemper) in triplicate and placed on the instrument’s capillary loading tray ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Biophysics 2020Quote: ... pH 8.2) was mixed with 10 µl of 300 µM NT647-N-hydroxysuccinimide fluorophore (NanoTemper Technologies) and incubated in the dark for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...
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bioRxiv - Biophysics 2021Quote: ... 10 μl of 100 nM labeled NHR2 in MST-buffer (included with the capillary set from NanoTemper) was mixed with 10 μl unlabeled NHR2 with concentrations ranging from 10.3 nM to 337.5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Thermophoresis data were analyzed when fluorescence signals lies within the 10% acceptance range using MO Affinity Analysis software V2.1.2 (Nanotemper). Data were plotted using GraphPad Prism 4.0 software and fitted with nonlinear equation.
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bioRxiv - Molecular Biology 2023Quote: ... The mixtures were incubated at room temperature for 10 min and transferred to 16 standard capillaries (Nanotemper, Germany). Data were acquired using medium MST power ...
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bioRxiv - Molecular Biology 2020Quote: ... 10-µL of undiluted rAAV of each lot was loaded into a high sensitivity capillary (Nanotemper Cat#PR-C006) and run on a Nanotemper Prometheus NT.48 nanoDSF instrument ...
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bioRxiv - Biochemistry 2019Quote: ... 10 μL of the different dilutions of samples were filled into hydrophilic treated capillaries (premium coated capillaries, NanoTemper Technologies) and measured after a 10 min equilibration at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... LLPS) were prepared in 1.5 ml Eppendorf tubes and loaded into 10 μl high sensitivity microcapillaries (NanoTemper Technologies, Germany) and subjected to DLS measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... a volume of 10 μl was then placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
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bioRxiv - Biochemistry 2023Quote: ... Approximately 10 µL of each sample was loaded into Prometheus NT.48 Series nanoDSF high sensitivity capillaries (NanoTemper, Germany) and melting scans were performed using the Pr.ThermControl software (NanoTemper ...
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bioRxiv - Bioengineering 2023Quote: ... The samples (10 µL at 0.2 mg/mL in HBS) were loaded into standard treated NT.115 capillaries (NanoTemper), and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper ...
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots (10 μl) of purified mTORC1_WT or mTORC1_EWEDdup variant were loaded into the Prometheus capillaries (Cat. PR-C002, NanoTemper Technologies), and the fluorescence intensity at 330 nm and 350 nm was recorded as a function of temperature from 10 °C to 90 °C ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Biochemistry 2022Quote: ... were done in the commercial MST buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2; Nanotemper Technologies) supplied with 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2021Quote: ... the mixtures were left to incubate for 10 min at room temperature and subsequently loaded in premium coated capillary tubes (NanoTemper). Measurements were performed with a Monolith NT.115 (NanoTemper ...
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bioRxiv - Molecular Biology 2023Quote: ... pH6) in a volume of 10 μl and placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were loaded into the MonoLith NT.115 standard treated capillaries and incubated for 10 min before analysis using the Monolith NT.115 instrument (NanoTemper Technologies) at 21°C using medium laser power and 40 % LED power ...
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bioRxiv - Cell Biology 2021Quote: ... proteins at 200 nM were incubated with varying concentrations of CaCl2 and ZnSO4 in a 10 µl mixture and loaded into NT.115 standard coated capillaries (NanoTemper Technologies). The buffers (25 mM HEPES ...
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bioRxiv - Plant Biology 2022Quote: ... The complex was allowed to establish for 10 min at room temperature before the samples were loaded into the capillaries (MO-K025, NanoTemper Technologies). Binding was detected by a Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... 13000 rpm), filled into Prometheus high-sensitivity capillaries (10 μL each, n=3) and transferred to a Prometheus Panta instrument (NanoTemper Technologies). DLS analysis of BbHtrA S/A rh and PDI were determined using Prometheus Panta’s size analysis function.
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bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...