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Citations for NanoTemper :
1 - 24 of 24 citations for 4 Phenyl 3 buten 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
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bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
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bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Microbiology 2020Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
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bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
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bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biochemistry 2022Quote: ... 13000 rpm), filled into Prometheus high-sensitivity capillaries (10 μL each, n=3) and transferred to a Prometheus Panta instrument (NanoTemper Technologies). DLS analysis of BbHtrA S/A rh and PDI were determined using Prometheus Panta’s size analysis function.
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bioRxiv - Microbiology 2022Quote: ... The labeled protein was 1:1 incubated with a series dilution of ABT199 (4 uM to 0.122 nM) to perform the microscale thermophoresis experiments in Monolith NT.115 (NanoTemper) with the setting of 40% MST power and 40% excitation power at 25 °C ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of EMC2•OG-488 was fixed at 50 nM and a dilution series of EMC8 or EMC9 ranging from 4 nM to 150 µM was used in the hydrophilic treated capillaries (NanoTemper Technologies). A Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermostability of MAPK14 was measured in the presence of different concentrations of MBZ in 50 mM HEPES buffer in the presence of 200 mM NaCl and 2% DMSO at pH 7.5 using a label-free fluorimetric analysis with a Prometheus NT.Plex instrument (NanoTemper Technologies), as described previously [52] ...
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bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...