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Citations for EpiCypher :
1 - 42 of 42 citations for Recombinant Human HHLA2 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Plant Biology 2024Quote: ... recombinant mononucleosomes (EpiCypher) as substrates ...
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bioRxiv - Molecular Biology 2023Quote: One μg of recombinant (Epicypher) or cellular nucleosomes (see protein purification above ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Biochemistry 2024Quote: ... One ug of recombinant designer demethylated nucleosomes (EpiCypher) was incubated with the PHF21A-containing complexes from 293T cells ...
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bioRxiv - Cell Biology 2021Quote: Recombinant semi-synthetic designer nucleosomes (dNucs) were from EpiCypher. Two phases of dCypher® testing on the PerkinElmer AlphaScreen® platform were performed as previously described9 ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated histone peptides for the dCypher assays were from EpiCypher (Resources Table A). Histone peptides for other studies were synthesized by the Peptide Core Facility at the University of Colorado Denver or by GenScript with a free N-terminus and an amidated C-terminus ...
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bioRxiv - Biochemistry 2022Quote: ... and 5’ biotinylated H3K4me3 187 bp mononucleosomes (16-2316) were purchased from Epicypher, Inc. ...
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bioRxiv - Cell Biology 2019Quote: ... 15 uL of recombinant nucleosomes (SNAP-ChIP, EpiCypher, 19-1001) were fixed with fresh 1% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: Biotinylated modified mononucleosomes containing either H3K9ac (16-0314) or H3K9me3 (16-0315) were purchased from Epicypher. To obtain nuclear extract from irradiated Kc cells ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed twice more before incubation with recombinant p-AG MNase (Epicypher 15-1016) at 4°C for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Biochemistry 2022Quote: ... a recombinant ATPase over a concentration range (Figure 2-figure supplement 1G-J) was mixed with 10 nM EpiDyne biotinylated nucleosome remodeling substrate (EpiCypher), terminally positioned 6-N-66 (219 bp fragment ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biophysics 2020Quote: ... Histone proteins were purchased from EpiCypher. While the H3 histones were available as part of recombinant human histone octamers ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments done with excess ubiquitinated nucleosome used purified recombinant mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-K120Ub) (EpiCypher, 16-0370) at 400 nM and 25 nM SAGA ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein A-micrococcal nuclease (pA-MN; EpiCypher donation) was added to a concentration of 1:400 to nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... we used protein A/G-MNase from Epicypher. For 24h pupal samples with 40 and 75 wings ...
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bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... The following commercial loaded protein A-Tn5 transposase fusion protein (pA-Tn5) were used at recommended dilutions by the manufacturer: CUTANA™ pAG-Tn5 (Epicypher 15-1017; Lot 20142001-C1), or pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins (0.4 μM) were mixed with nucleosome (50N66, 0.03 μM - Epicypher) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). Nucleosome reconstitutions were performed by salt gradient dialysis64 ...
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bioRxiv - Biochemistry 2022Quote: ... coli (ISWI, ModT antigen and LCMS reference proteins) or obtained from EpiCypher Inc ...
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bioRxiv - Biophysics 2024Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). We followed the previously published protocol to prepare nucleosome reconstitutions [80] ...
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... The pAG-Tn5 fusion protein used in many of these experiments was a gift from Epicypher, Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... This was followed by washing and incubation with the protein A/G conjugated Tn5 (CUTANA pAG-Tn5, Epicypher) enzyme for one hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell-bead slurry was washed twice with Digitonin Buffer and incubated with 1x Protein-A/G-MNase (pAG-MNase; Epicypher) in Digitonin Buffer for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed in Wash buffer 300 (Wash buffer containing 300mM NaCl) two times and resuspended in Wash buffer 300 containing Protein A/G-TN5 (EpiCypher) and incubated for an hour at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...