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Citations for EpiCypher :
1 - 40 of 40 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Plant Biology 2024Quote: ... recombinant mononucleosomes (EpiCypher) as substrates ...
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bioRxiv - Cancer Biology 2021Quote: ... After overnight incubation at 4°C with the primary FOXA1 antibody (13-2001, Epicypher), cell-bead slurry was washed twice more after which pA-MNase digestion was activated by placing samples on an ice-cold block and incubated with digitonin wash buffer containing 2 mM CaCl2 ...
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bioRxiv - Molecular Biology 2023Quote: One μg of recombinant (Epicypher) or cellular nucleosomes (see protein purification above ...
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bioRxiv - Biochemistry 2024Quote: ... One ug of recombinant designer demethylated nucleosomes (EpiCypher) was incubated with the PHF21A-containing complexes from 293T cells ...
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bioRxiv - Immunology 2022Quote: ... then resuspended and incubated at 4°C for 1 hour in CUT&RUN antibody buffer and 2.5 μL pAG-MNase (EpiCypher). ConA-bound-nuclei were then washed twice with CUT&RUN triton wash buffer ...
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bioRxiv - Systems Biology 2023Quote: ... an input of ∼400,000 cells per antibody condition and an overnight antibody incubation at 4°C with H3K4me3 (0.5 μg/ml, EpiCypher), H3K9me3 (0.9 μg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissected tissues were dissociated in 100µg/mL Liberase at 37°C for 10minutes and processed using EpiCypher’s CUTANA kit reagents (EpiCypher 15-1016) and protocol with the following modifications ...
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bioRxiv - Immunology 2024Quote: ... Cells were then permeabilized and incubated overnight on a nutator at 4°C with 1 μg of normal rabbit IgG (EpiCypher), KLF4 (proteintech ...
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bioRxiv - Cell Biology 2021Quote: Recombinant semi-synthetic designer nucleosomes (dNucs) were from EpiCypher. Two phases of dCypher® testing on the PerkinElmer AlphaScreen® platform were performed as previously described9 ...
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bioRxiv - Cell Biology 2019Quote: ... 15 uL of recombinant nucleosomes (SNAP-ChIP, EpiCypher, 19-1001) were fixed with fresh 1% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated overnight (4°C with gentle rocking) with 1 µg of antibody (For all 40 PCRP antibodies as above; RbIgG (EpiCypher 13-0042, lot 20036001-52); MsIgG (Invitrogen 10400C ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed twice more before incubation with recombinant p-AG MNase (Epicypher 15-1016) at 4°C for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments done with excess ubiquitinated nucleosome used purified recombinant mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-K120Ub) (EpiCypher, 16-0370) at 400 nM and 25 nM SAGA ...
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bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 10 min with Concanavalin A-conjugated magnetic beads (Epicypher) that were previously activated by repeated washings with a buffer containing 20 mM HEPES pH 7.9 ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then transferred to an 8-strip tube (EpiCypher 10-0009). Each sample was incubated with 0.5 µg primary or IgG control antibody (Supplemental Table 5 ...
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bioRxiv - Genomics 2023Quote: 10-20ng of W601 sequence (free and histone bound, cat. 16-0009, Epicypher) was combined with antibody in 200μL of PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% BSA) and 10 µL per antibody treatment of ConA-coated magnetic beads (Epicypher) added with gentle vortexing ...
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bioRxiv - Genetics 2022Quote: ... Thermo Scientific 78425) for 10 minutes on ice and immobilized onto Concanavalin-A beads (EpiCypher 21-1401). After blocking and washes ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 nM mononucleosomes (H3K9me0 and H3K9me3, Epicypher catalog nos. 16-0006 and 16-0315-20) constant in binding buffer [20 mM Hepes (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: Beads were prepared using 10 ul/sample of CUTANA Concavalin A Conjugated Paramagnetic Beads (EpiCypher, SKU:21-1401). Beads were transferred to a 1.5 ml tube and placed in a magnetic separation rack ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts, Epicypher Cat# 13-0031), Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns ...
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bioRxiv - Cancer Biology 2024Quote: A total of 500,000 cells per reaction were harvested and captured using 10 µL of pre-activated ConA beads (EpiCypher). Beads with attached cells were incubated at room temperature for 10 min to ensure complete adsorption ...
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bioRxiv - Biochemistry 2022Quote: ... a recombinant ATPase over a concentration range (Figure 2-figure supplement 1G-J) was mixed with 10 nM EpiDyne biotinylated nucleosome remodeling substrate (EpiCypher), terminally positioned 6-N-66 (219 bp fragment ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... Two microliters of uniquely barcoded i5 and i7 primers (10 μM) and 25 μl CUTANA High Fidelity 2x PCR Master Mix (EpiCypher, #15-1018) were added to each sample and mixed ...
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bioRxiv - Genetics 2022Quote: ... loaded with Illumina NEXTERA adapters (a kind gift from the Henikoff lab, via Madapura lab, diluted 1:250, or Epicypher CUTANA® pAG-Tn5, diluted 1:10) was prepared in Dig-300 Buffer (0.05% Digitonin ...