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Citations for EpiCypher :
1 - 43 of 43 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Differential SMARCC1 binding between E11.5 (40-43s) control (Gli3+/+; n=3) and Gli3-/- (n=4) samples was conducted using an E.coli spike-in (EpiCypher 18-1401) of 0.125ng/sample ...
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bioRxiv - Systems Biology 2023Quote: ... an input of ∼400,000 cells per antibody condition and an overnight antibody incubation at 4°C with H3K4me3 (0.5 μg/ml, EpiCypher), H3K9me3 (0.9 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL i5 universal primer (EpiCypher), 2 µL i7 barcoded primer (EpiCypher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL i7 barcoded primer (EpiCypher), 0.005 ng Escherichia coli spike-in DNA (made in-house) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... One ug of recombinant designer demethylated nucleosomes (EpiCypher) was incubated with the PHF21A-containing complexes from 293T cells ...
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bioRxiv - Genomics 2022Quote: ... we added 4 ul of SNAP-ChIP K-MetStat Panel (EpiCypher) to every 10 ug of chromatin ...
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bioRxiv - Biophysics 2023Quote: H3K27ac nucleosomes were assembled using the LIN28B DNA (unlabeled or Cy5-labeled) and histone octamer with H3K27ac modification (custom purchased from Epicypher). For H3K27-trimethyl nucleosomes ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg/mL Glycogen with E.coli spike in control (Epicypher, 18-1401)).
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bioRxiv - Cancer Biology 2021Quote: ... After overnight incubation at 4°C with the primary FOXA1 antibody (13-2001, Epicypher), cell-bead slurry was washed twice more after which pA-MNase digestion was activated by placing samples on an ice-cold block and incubated with digitonin wash buffer containing 2 mM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... and 5’ biotinylated H3K4me3 187 bp mononucleosomes (16-2316) were purchased from Epicypher, Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... a 1:5 dilution of K-MetStat Panel spike-in nucleosomes (EpiCypher, cat. # 19-1002) was added to each reaction prior to addition of antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of SNAP-ChIP K-MetStat panel and K-AcylStat panel nucleosomes (EpiCypher) respectively ...
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bioRxiv - Immunology 2022Quote: ... then resuspended and incubated at 4°C for 1 hour in CUT&RUN antibody buffer and 2.5 μL pAG-MNase (EpiCypher). ConA-bound-nuclei were then washed twice with CUT&RUN triton wash buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were then permeabilized and incubated overnight on a nutator at 4°C with 1 μg of normal rabbit IgG (EpiCypher), KLF4 (proteintech ...
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bioRxiv - Genetics 2023Quote: ... 2 μl /1×106 cells of the following antibodies were used for immunoprecipitations: H3K4me3 (EpiCypher 13-00041), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Biochemistry 2023Quote: All PTM-defined nucleosomes (Supplementary file 5; homotypic unless stated otherwise) were from the dNucTM or versaNuc® portfolios (EpiCypher). PTMs were confirmed by mass-spectrometry and immunoblotting (if an antibody was available ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041, 1:50], or rabbit IgG negative control antibody [Epicypher, 13-0042, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Biochemistry 2022Quote: ... a recombinant ATPase over a concentration range (Figure 2-figure supplement 1G-J) was mixed with 10 nM EpiDyne biotinylated nucleosome remodeling substrate (EpiCypher), terminally positioned 6-N-66 (219 bp fragment ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated overnight (4°C with gentle rocking) with 1 µg of antibody (For all 40 PCRP antibodies as above; RbIgG (EpiCypher 13-0042, lot 20036001-52); MsIgG (Invitrogen 10400C ...
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bioRxiv - Molecular Biology 2024Quote: ... beads were washed twice with 200 µl 0.25 % Digitonin wash buffer and resuspended in Digitonin wash buffer containing 2 µl CUTANA™ pAG-MNase (15-1016, EpiCypher, 15-1016) and incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina sequencing libraries were prepared from ∼5 ng of purified DNA using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001 & 14-1002) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... anti-BRG1 (13-2002, EpiCypher, 1:50 dilution), and non-immune rabbit IgG (2729 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-H3K4me3 (13-0041, EpiCypher, 1:50 dilution), anti-H3K27me3 (9733 ...
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bioRxiv - Bioengineering 2023Quote: ... Next each of 3 100ul aliquots (∼1/3 of each 24 well) of cells were processed for H3K4me3 antibody (Epicypher, #13-0041), H3K27ac antibody (Epicypher ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended 50 µg of 601 nucleosome positioning sequence DNA (EpiCypher) in 50 µL nuclease-free 2M NaCl ...
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bioRxiv - Developmental Biology 2023Quote: ... 137 was performed using the CUTANA ChIC / CUT&RUN Kit Version 3 (EpiCypher, 14-1048) and CUTANA CUT&RUN Library Prep Kit (EpiCypher ...
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bioRxiv - Systems Biology 2023Quote: ... and IgG (0.5 μg/ml, EpiCypher) antibodies ...
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were resuspended in 50 μl secondary antibody buffer (goat anti-rabbit IgG (Epicypher, 13-0047) diluted 1:100 in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... H3K4ME3 (EpiCypher, 13-0041K, 0.5 mg/ml), BRD4 (Cell signaling ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were washed following the ChIC/CUT&RUN Kit Version 3 (EpiCypher, Inc, Catalog No. 14-1048) user manual before the binding to Ty1 BB2 MAb ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% digitonin) and resuspended in 50 μl cold Dig-Wash buffer with 1 μl pAG-MNase (EpiCypher, 15-1016) per reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... and IgG (EpiCypher, 13-0042K, 0.5 mg/ml). The cells were then incubated overnight on a nutator at 4 °C and permeabilized using a buffer containing 5% digitonin ...
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bioRxiv - Biochemistry 2023Quote: ... the permeabilized nuclei were divided equally and incubated in 50 µL antibody buffer with either 0.5µg of control rabbit IgG antibody (Epicypher 14-1048) or anti-c-Myc antibody (ThermoFisher Scientific 13-2500 ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5μg of rabbit glucocorticoid receptor antibody [abcam, ab225886, 1:20] or rabbit IgG negative control antibody [Epicypher, 13-0041k, 1:50]). The remaining steps were performed according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... bead-cell complexes were washed twice with 200 μl cold dig-washing buffer (washing buffer supplemented with 0.01% digitonin) and resuspended in 50 μl cold dig-washing buffer with 1 μl pAG-MNase (EpiCypher, Cat #15-1016). After incubation at room temperature for 10 min ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to each sample (RELA antibody [Santa Cruz, sc-109, 1:25] or rabbit IgG negative control antibody [EpiCypher, 13-0041, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended in 50μL Digitonin Buffer containing 700ng/mL CUTANA pAG-MNase (Epicypher), and incubated for 10 mins at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissected tissues were dissociated in 100µg/mL Liberase at 37°C for 10minutes and processed using EpiCypher’s CUTANA kit reagents (EpiCypher 15-1016) and protocol with the following modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of nucleosomes is maintained above 20 μg/ml to avoid dissociation due to dilution38 using commercial nucleosomes (Epicypher #160009). Final constructs were dialyzed overnight against 25 mM HEPES pH 7.6.