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Citations for Avidity :
1 - 28 of 28 citations for Human Serum Albumin Rabbit Polyclonal affinity purified since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... coli lysates by Ni-NTA affinity chromatography followed by site-specific biotinylation using BirA biotin-protein ligase (Avidity). The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Secreted protein was purified using HisPur Ni-NTA resin (Thermo) for immobilized metal affinity chromatography (IMAC) and biotinylated overnight at 4°C using biotin (Avidity), ATP ...
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bioRxiv - Immunology 2020Quote: ... Purified RBDhisavi was biotinylated in vitro using BIRA (Avidity.com) for subsequent formation of RBDhisavi-tetramers for B-cell staining experiments ...
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bioRxiv - Bioengineering 2022Quote: ... Purified samples were biotinylated using BirA biotin ligase (Avidity Inc.) at 1 µg of BirA enzyme per 10 nM of protein.
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bioRxiv - Immunology 2022Quote: ... Purified pMHC was biotinylated using the BirA enzyme (Avidity, USA).
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bioRxiv - Immunology 2022Quote: ... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... purified fractions were biotinylated using a BirA protein ligase kit (Avidity LLC). The biotinylated proteins were further purified by gel filtration (S200 ...
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bioRxiv - Biophysics 2022Quote: ... coli.19 The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Avi-Tagged FcRs (Duke Human Vaccine Institute) were biotinylated using BirA500 kit (Avidity) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: The purified protein was biotinylated by BirA using a BirA-500 kit (Avidity). Typically ...
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bioRxiv - Microbiology 2021Quote: ... Purified SARS2 D614G spike protein was biotinylated using BirA500 biotinylation kit from Avidity. To 50 ug of spike protein ...
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bioRxiv - Immunology 2019Quote: ... Purified peptide-HLA-A*02 was biotinylated in vitro by BirA enzyme (Avidity) and mixed with Streptavidin:RPE (Bio-Rad #STAR4A ...
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bioRxiv - Immunology 2024Quote: ... Avi-tagged Rhesus macaque FcγRs (Duke Human Vaccine Institute) were biotinylated using BirA500 kit (Avidity) per manufacturer’s instructions and tagged with streptavidin-PE ...
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bioRxiv - Biochemistry 2022Quote: ... the purified RBDs were biotinylated using the BirA biotin-protein ligase reaction kit (Avidity). The biotinylated proteins were re-purified and concentrated as described above ...
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bioRxiv - Immunology 2020Quote: ... purified PR8-HA and S proteins were biotinylated using BirA biotin-protein ligase (Avidity). Biotinylated PR8-HA and S proteins were fluorescently labelled by the sequential addition of streptavidin-conjugated to phycoerythrin (PE ...
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bioRxiv - Immunology 2020Quote: ... purified spike ectodomain trimers were biotinylated by addition of biotin-protein ligase (Avidity, Aurora, CO). Biotinylated spike ectodomain trimers were buffer exchanged into PBS ...
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bioRxiv - Immunology 2024Quote: ... The purified spike protein was biotinylated using the BirA biotin-protein ligase reaction kit (Avidity) following the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2023Quote: Biotinylation of purified Hsc70 was performed using the BirA bulk kit according to manufacturer’s protocol (Avidity LLC). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylation of the AviTag in full-length human FLCN-8xG-AviTag-PreScission-MBP was performed following according to the manufacturer’s (Avidity) suggested protocol and was performed following PreScission protease cleavage and prior to size exclusion chromatography ...
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins with an avi-tag were biotinylated by using BirA following the BirA500 kit’s protocol (Avidity, BirA500). Biotinylation was confirmed by performing a Coomassie gel shift assay according to Fairhead and Howarth ...
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bioRxiv - Immunology 2020Quote: ... Avi-tagged HAs were expressed and purified as described above and biotinylated using the Biotin ligase kit (Avidity) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Purified mini-HA was biotinylated using the Biotin-Protein Ligase-BIRA kit according to the manufacturer’s instructions (Avidity). Biotinylated mini-HA was then conjugated to streptavidin– PE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... purified spike ectodomain trimers and RBD domain protein were biotinylated by the addition of biotin-protein ligase (Avidity). Biotinylated proteins were buffer exchanged into PBS ...
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bioRxiv - Immunology 2023Quote: ... rhesus macaque FcγR2A and FcγR3A (acquired from the Duke Human Vaccine Institute Protein Production Facility) were biotinylated with a BirA biotin–protein ligase bulk reaction kit (Avidity). C1Q (Sigma ...
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bioRxiv - Immunology 2021Quote: ... spike protein probes were added one-by-one to FACS wash buffer (1x PBS, 2% fetal bovine serum) containing 5μM free d-biotin (Avidity, Cat. Bir500A). Both streptavidin-fluor conjugates were used to stain DMSO control samples to further verify the absence of significant frequencies of non-specific streptavidin-binding B cells.
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bioRxiv - Bioengineering 2019Quote: ... The recombinant extracellular domain of CD38 and PD-L1 was produced as a 6-His and AviTag™ fusion in HEK293 cells and purified by Ni-NTA followed by in vitro biotinylation by BirA biotin ligase (Avidity).
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bioRxiv - Biochemistry 2021Quote: ... 750 µg of purified uncleaved hSPAK-CCT or hOSR-CCT were mixed with 50 µl of SuperMix (containing ATP and D-biotin, Avidity, LLC) and 5 μg of BirA (Avidity ...
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bioRxiv - Synthetic Biology 2023Quote: Proteins with Avi-tags (GLNDIFEAQKIEWHE; see supplementary materials) were purified as described above and biotinylated in vitro using the BirA500 (Avidity, LLC) biotinylation kit ...