Labshake search
Citations for KeraFAST :
1 - 13 of 13 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
-
bioRxiv - Microbiology 2020Quote: ... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
-
bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
-
bioRxiv - Immunology 2023Quote: ... and the pcDNA3.1-spike protein plasmids and G*ΔG-VSV (Kerafast) with firefly luciferase in place of VSV-G were transfected into 293T cells (American Type Culture Collection [ATCC] ...
-
bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
-
bioRxiv - Microbiology 2023Quote: ... a BlueScript backbone plasmid under T7 polymerase promoter control encoding VSV Indiana backbone in which the VSV glycoprotein (G) gene has been replaced with GFP (pVSV-ΔG-GFP) was purchased from Kerafast. The pVSV-ΔG-GFP was modified by cloning the codon optimized ORF of RABV G protein of Egyptian strain (GenBank accession number MK760770.1 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293T/Spike cells were incubated with pseudotyped G*ΔG-GFP rVSV (Kerafast EH1024-PM) at MOI ∼3 for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... Keigi Fujiwara (University of Texas MD Anderson Cancer Center, Houston, TX) and IP3R1-null HEK293 cells (Kerafast, Inc., Boston, MA) (Alzayady ...
-
bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
-
bioRxiv - Immunology 2022Quote: SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was generated by nucleofection of BHK cells (maintained in DMEM/F12 with 10%FBS and 5%TPB) with Spike-expressing plasmid followed by transduction with G-pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast) 18-24 hours later ...
-
bioRxiv - Immunology 2023Quote: ... CRL-3216) was transfected with the spike protein-expressing plasmids and then infected with G*ΔG-VSV virus (Kerafast, EH1020-PM). After culturing ...
-
bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...