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Citations for KeraFAST :
1 - 39 of 39 citations for Human Neuropilin 2 NRP 2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
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bioRxiv - Microbiology 2022Quote: ... BHK21/WI-2 cells (Kerafast #EH1011) were maintained in DMEM (Thermo Fisher #11965092 ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S-expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described.6 For the neutralization assay ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S expression plasmid and subsequently infected with VSV△G-firefly-luciferase as previously described [40] ...
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bioRxiv - Immunology 2021Quote: ... BHK21/WI-2 cells (Kerafast, EH1011) were transfected with the Wuhan-1 strain (Genbank # ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) were maintained in DMEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast ...
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bioRxiv - Immunology 2021Quote: ... BHK-21/WI-2 cells (Kerafast, EH1011) were transfected with the spike expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described (Whitt ...
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bioRxiv - Biochemistry 2023Quote: ... 20% glycerol] and 2 µL of Marathon (Kerafast) were added to the solution ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
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bioRxiv - Immunology 2022Quote: VSV pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously.66 BHK-21/WI-2 cells were plated into a 6 well plate ...
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bioRxiv - Immunology 2022Quote: VSV vectors pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously [51] ...
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bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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bioRxiv - Microbiology 2020Quote: ... and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene ...
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bioRxiv - Immunology 2021Quote: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011), African Green monkey kidney-derived Vero E6 cell line (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells were purchased from Kerafast (Boston, MA, USA) and cultured in D5+ media ...
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bioRxiv - Microbiology 2022Quote: Baby hamster kidney cells (BHK-21/WI-2, Kerafast # EH1011, Boston, MA, USA) and African Green Monkey Cell Line (VeroE6 ...
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bioRxiv - Immunology 2021Quote: ... and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Microbiology 2020Quote: ... and were infected 2 days post-transfection with >107 infectious units/well of VSVΔG-GFP (Kerafast, (22)) ...
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bioRxiv - Immunology 2021Quote: ... 2021) and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Immunology 2020Quote: ... HEK293T/17 monolayers transfected to express SARS-CoV-2 S truncated of the C-terminal cytoplasmic 19 amino acids (SARS-CoV-2-S-CΔ19) were inoculated with VSVΔG-GFP vector (rescued from pVSVΔG-GFP plasmid expression vector; Kerafast Inc.). After incubation for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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bioRxiv - Microbiology 2020Quote: ... Cells were rinsed with PBS one hour after infection and replace with complete media supplemented with 2 microliters of mouse hybridoma supernatant containing anti-VSV-G antibody I1 (Kerafast) to neutralize input virus ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were gently washed three times 2-hour post-infection and incubated in DMEM containing 1:1000 dilution of anti-VSV-G antibody (Kerafast: 8G5F11) to eliminate residual ΔG-VSV particles at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Reporter gene Renilla luciferase was cloned into the multiple cloning site MCS-2 of plasmid pVSV-ΔG-N/P-MCS2-2.6 (Kerafast, Boston Massachusetts), which encodes the positive-sense RNA of a replication-defective recombinant vesicular stomatitis virus (rVSV ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were subsequently briefly rinsed thrice with 2% BSA/0.05% Tween (in PBS) and incubated with mouse anti-DNA– RNA hybrid S9.6 (Kerafast, ENH001; 1:100) and rabbit anti-nucleolin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... Pseudoviruses were generated in BHK-21/WI-2 cells using a pseudotyped ΔG-GFP (G*ΔG-GFP) rVSV (Kerafast, Boston, MA, USA) in addition to spike sequences cloned into the paT7- Spike plasmid as described previously (21) ...
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bioRxiv - Microbiology 2019Quote: ... Human Pancreatic Ductal Epithelial (HPDE) cells (Kerafast H6c7) were maintained in keratinocyte SFM (serum-free medium ...
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bioRxiv - Genomics 2023Quote: The U-251 MG human cells (Kerafast, AccessionID: CVCL_0021) stably expressing dCas9-VPR were cultured in T150 tissue culture flasks (TPP ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal human pancreatic duct H6c7 cells were purchased from Kerafast, Inc ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... Normal human pancreatic duct cell line H6c7 was obtained from Kerafast, Inc ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UNCN1T cells (a human bronchial epithelial cell line; Kerafast; cat# ENC011) were cultured using BEGM media (Bronchial Epithelial Cell Growth Medium ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-human procollagen type 1 antibody (LF68, ENH018) was purchased from Kerafast Inc ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used include: human polyclonal anti-Zika virus antibody (Kerafast EVU302, MA) or mouse anti-ZikV E protein mAb (Fitzgerald ...
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bioRxiv - Cell Biology 2020Quote: Triple IP3R knockout human embryonic kidney cell line (TKO-HEK) from Kerafast (Boston, MA, USA; #EUR030) was grown in DMEM (#11995-065 ...
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bioRxiv - Cell Biology 2024Quote: ... we utilized a human immortalized (HuIm) RPTEC line (Clone TH1, passage 8, Cat No. ECH001, Kerafast, Boston, MA), as control ...
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human FN III 12,13 N-GST (“HBDII”, which is derived from heparin-binding domain II of FN; #EUR120, Kerafast). Sheared DNA molecules were then added dropwise with continuous stirring at 400∼500 rpm with the final concentration of 10 µg/ml ...