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Citations for KeraFAST :
1 - 41 of 41 citations for C Reactive Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... L and G proteins (Kerafast), all of which under T7 promoter control ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was expressed in LOBSTR (Kerafast) E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-Matrix Protein (Kerafast, Inc.; EMS009) or goat anti-Influenza A Virus (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... We incubated 48h at 4°C with rat anti-tdTomato (16D7, Kerafast) diluted 1:500 in a solution of 3% bovine albumin serum and 0.3% Triton X-100 prepared in PBS 1x ...
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bioRxiv - Immunology 2022Quote: ... Membranes were probed overnight at 4°C with anti-RNA:DNA hybrid (S9.6) (Kerafast) at 1:10,000 dilution or dsDNA antibody (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... eEF3 protein was detected with anti-eEF3 antibody (Kerafast ED7003) and Glucose-6-phosphate dehydrogenase (G-6-PDH ...
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bioRxiv - Microbiology 2020Quote: ... rat anti-MLV transmembrane protein/p15E (clone 42/114, Kerafast), rabbit anti-myc (Cell Signaling Technology) ...
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bioRxiv - Microbiology 2019Quote: ... IAV matrix protein monoclonal antibody (E10; catalog no. EMS009, Kerafast) at 1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
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bioRxiv - Microbiology 2022Quote: ... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
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bioRxiv - Immunology 2023Quote: ... and the pcDNA3.1-spike protein plasmids and G*ΔG-VSV (Kerafast) with firefly luciferase in place of VSV-G were transfected into 293T cells (American Type Culture Collection [ATCC] ...
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bioRxiv - Genetics 2023Quote: ... Newly synthesized proteins labeled with 1:1000 mouse α puromycin (Kerafast, EQ0001) and normalized to Ponceau staining ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated O/N at 4°C with a monoclonal S9.6 antibody (Kerafast, 1:100) diluted in 3%PBS-BSA ...
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bioRxiv - Microbiology 2021Quote: ... anti-matrix protein [E10] (M1 and M2, 1:1,000, Kerafast cat. no. EMS009), and anti-SARS-CoV-2 spike protein (RBD ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated overnight at 4°C with anti-M2 monoclonal antibody (E10; catalog no. EMS009, Kerafast). Three washes were performed prior to addition of goat anti-mouse Alexa Fluor-647 secondary antibody (catalog no ...
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bioRxiv - Genomics 2022Quote: ... 275 mM NaCl) and incubated overnight at 4°C in the presence of 1.5 µg of S9.6 purified antibody (Kerafast). Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the following primary antibodies overnight at 4°C for S9.6-HIV-1-IN_PLA: mouse anti-DNA–RNA hybrid S9.6 (1:250; Kerafast, ENH001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... together with Anti-Influenza A Non-Structural Protein 1 (NS1) Mouse Monoclonal Antibody (EMB005, Kerafast). Cells were then washed and incubated with a mixture of secondary antibodies ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight on a rotating wheel at 4 °C in presence of 1 μg of S9.6 antibody (Kerafast) and 20 μl of protein G sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37 °C with 8% CO2 and VSV-G pseudo-typed DG-luciferase (G*DG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1xDPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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bioRxiv - Microbiology 2023Quote: ... After 1.5 h at 37°C cells were washed with PBS and incubated with DMEM containing 1 μg/ml anti-VSV-G antibody (Kerafast, EB0010) for 24 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was evaluated as FFU/mL using the anti-VSV-Matrix protein 23H12 antibody (Kerafast Inc.) to detect infected cells.
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bioRxiv - Microbiology 2021Quote: ... VSV M protein was detected using anti-VSV M antibody clone [23H12] (KeraFast, Boston, MA, Cat.# EB0011) at 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and protein synthesis level was determined by Western blot using anti-puromycin antibody (KeraFAST, Cat#EQ0001, 1:1000). The combined intensity of proteins ranging from 20 to 200 kDa was quantified and normalized to the level of β-actin.
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bioRxiv - Molecular Biology 2021Quote: ... while the lower portion of the membrane was probed with anti-VSV matrix protein antibody (Kerafast, EB0011; loading control) diluted 1:2,500 in 5% skim milk solution ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses bearing mutant S proteins were produced using the ΔG luciferase recombinant vesicular stomatitis virus (rVSV) system (KeraFast EH1025-PM)(Whitt ...
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bioRxiv - Neuroscience 2020Quote: Detection of newly synthesized proteins by proximity ligation was performed as previously described6 ssing a mouse anti-puromycin (Kerafast, 1:500) antibody in combination with rabbit anti-beta actin (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The denatured TCA-treated proteins were subjected to immunoblot analysis for VSV M and EBOV GP protein levels using anti-VSV M (1:2500, Kerafast, EB0011) and anti-EBOV GP (1:1000 ...
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bioRxiv - Physiology 2020Quote: In vivo muscle protein synthesis was assessed using the non-isotopic SUnSET method and an antibody against puromycin (Kerafast, Boston, MA), as described previously [31 ...
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bioRxiv - Physiology 2020Quote: ... Protein synthesis was determined in the freely fed state using the SUnSET method and an antibody against puromycin (Kerafast, Boston, MA)25,26 ...
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bioRxiv - Immunology 2023Quote: ... CRL-3216) was transfected with the spike protein-expressing plasmids and then infected with G*ΔG-VSV virus (Kerafast, EH1020-PM). After culturing ...
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bioRxiv - Neuroscience 2019Quote: Detection of newly synthesized proteins by proximity ligation was carried out using anti-puromycin antibodies (mouse anti-Puromycin from Kerafast, 1:500-1000) in combination with protein-specific antibodies (rabbit anti-RapGEF4 from Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...