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Citations for PolyScience :
1 - 35 of 35 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... plasmid using PEI MAX (Polyscience). For small or large AAV preparations ...
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bioRxiv - Biochemistry 2021Quote: Plasmid transfections were conducted using polyethyleneimine (Polyscience) according to the standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the rep-cap AAV8 helper plasmid and an adenoviral helper plasmid was mixed and transfected using polyethylenimine (PEI) (Polyscience, USA). The cells were harvested in 3 ml of lysis buffer and lysed by four freeze–thaw cycles 72 hours after transfection ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 μg U6-ELS77 plasmids using polyethylenimine (Polyscience Inc.). Transfected cells were switched into Optimem (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... *SIM or *RING expression plasmids using PEI transfection reagent (Polyscience Inc). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µg lentiviral plasmid using PEI-Max (Polyscience, cat.no. 24765-1). Lentiviral supernatant was harvested 36 ...
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bioRxiv - Biochemistry 2021Quote: ... pcDNA-PB1 and wild-type or mutant pcDNA-PB2 plasmids using PEI (Polyscience) and Opti-MEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... by co-transfection of heavy and light chain expressing plasmids using polyethylenimine (Polyscience) and cells were cultured at 37 °C with shaking at 125 rpm and 8% CO2 ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.G (100 ng/well) plasmids using PEI MAX (Polyscience, 24765-100). Cells were incubated at 37C ...
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bioRxiv - Biochemistry 2021Quote: ... pcDNA-NP and pPOLI-NA-RT plasmid encoding neuraminidase (NA) vRNA segment using PEI (Polyscience) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with Strep-HA-VCF1 expression plasmids using PEI transfection reagent (Polyscience Inc). After 30 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 800 ng of mHilpda_mCherry plasmid complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were transfected with expression and packaging plasmids following standard calcium phosphate or polyethylenimine (Polyscience, 23966) protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then transfected with either Reelin-HA or control plasmid using 1.0 mg/ml polyethyleneimine MAX (Polyscience). After 2 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Cell Biology 2021Quote: ... This plasmid was then transfected into immortalized STING-knockout MEFs (Mukai et al., 2016) with PEI MAX (24765-1, Polyscience). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...
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bioRxiv - Microbiology 2020Quote: A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Cancer Biology 2020Quote: ... Retroviral particles were produced in HEK293T cells after co-transfection of retrovirus plasmid vector pWZL-Blast-Flag-Snail or control vector pWZL-Blast-Flag-Empty with packaging plasmids (VSVG, Gag/pol) using polyethylenimine (PEI) (Polyscience). After 48h and 72h ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with pLIB2-BSD plasmid bearing cDNA of human CLPTM1L or CLPTM1 by using PEI “Max” transfection reagent (Polyscience) and cultured for 12 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a total of 800 ng of plasmids encoding both Target-AID and gRNA were combined with 2.5 µL of 1 mg/mL PEI MAX (Polyscience #24765) and 200 µL of 1x PBS and applied to each well after 5–10 min incubation at room temperature.
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bioRxiv - Microbiology 2023Quote: HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
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bioRxiv - Immunology 2023Quote: ... and all plasmid constructs used throughout the study were transfected into cells using PEI transfection reagent (Polyscience, Cat; 24765-1) at a concentration of 1□µg/ml ...
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bioRxiv - Biophysics 2023Quote: ... We transfected HeLa cells (ATCC CCL-2) with 10 µg of each px459 v2.0 plasmid together with 40 µl of linear PEI (Polyscience 23966) using standard protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids containing shRNA or corresponding empty vector were cotransfected with lentivirus packaging plasmids (pLP1, pLP2, and pLP/VSVG) into HEK293T cells using linear polyethylenimine (23966, Polyscience) transfection method ...
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bioRxiv - Immunology 2022Quote: The heavy and light chain plasmids were then co-transfected into HEK293 cells us-ing PEI MAX® (Cat: 24765-1, PolyScience). The production supernatant was harvested af-ter 14 days post-transfection and purified using AKTA equipped with a PpL affinity col-umn (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... and AAV rep and cap plasmid for the construction PHP.eB capsid were co-transfected into HEK293T cells using polyethyleneimine (catalogue no. 24765; Polyscience, Inc., Warrington, PA). The cells and culture media were separately harvested 72 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and the pHGT1-Adeno1 helper plasmid carrying adenoviral genes (kindly provided by C. Cepko, Harvard Medical School, Boston, USA) using PEIMAX (Polyscience, #POL24765-1). Plasmids were mixed in 98 mL DMEM (Thermo Fischer ...
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bioRxiv - Biochemistry 2020Quote: ... Transfections were performed with 800 ng of single or 1600 ng of mixed plasmid DNA complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNAs were introduced into Lenti-X 293T or HEK293T cells using either polyethylenimine hydrochloride (PEI) (49553-93-7, Polyscience, Niles, IL, USA) or TransIT®-293 Transfection Reagent (MIR2700 ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...