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Citations for PolyScience :
1 - 46 of 46 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and pMD2.G (100 ng/well) plasmids using PEI MAX (Polyscience, 24765-100). Cells were incubated at 37C ...
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bioRxiv - Microbiology 2021Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Developmental Biology 2021Quote: ... En bloc staining was done in the dark with 1% UA (cat# 21447, Polyscience Europe GmbH) in water at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells were transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Microbiology 2020Quote: A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
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bioRxiv - Cell Biology 2021Quote: ... 4.5 μl X-tremeGENE 9 in 100 μl serum-free OptiMEM) or PEI transfection reagent (1 mg ml−1 stock, Polyscience 24765-2 ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: ... or 100 pmol/mL of SC-siRNA and linear polyethylenimine (PEI) with a molecular weight of 25 kDa (23966-1; Polyscience, USA) in a 1:3 ratio (μg of siRNA ...
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bioRxiv - Molecular Biology 2024Quote: 1.6 µg of expression plasmid DNA in 100 µl of 150 mM NaCl were mixed with 100 µl of 150 mM NaCl supplemented with 7 µl of 1 mg/ml PEI (polyethylenimine ‘MAX’ Polyscience, Inc.). After 30 min incubation at a room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Transfections were performed with 800 ng of single or 1600 ng of mixed plasmid DNA complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Immunology 2023Quote: ... using Polyethylenimine (PolyScience 23966-100) or FuGENE (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 4% v/v formaldehyde (methanol-free; Polyscience) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... Transfection was conducted with 4 μl of PEI (Polyscience) following the manufacturer’s manual and 1μg of CR plasmids and 1μg of rgRNA plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... root meristems were excised and fixed in 4% formaldehyde (Polyscience) in PBS pH 7.2 for 24 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 800 ng of mHilpda_mCherry plasmid complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum-free DMEM ...
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bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... 4.5 μm fluorescent beads (100 beads/μL, YG-beads, Polyscience) were added to the sample as an internal particle concentration standard ...
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bioRxiv - Biochemistry 2020Quote: ... Next day cells were transfected with 750 ng of Hilpda_sYFP2 complexed to polyethylenimine (PEI) (Polyscience Inc., PA, USA) in serum free medium ...
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bioRxiv - Synthetic Biology 2023Quote: ... for 24 hours after which transfection took place using 187.5 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transiently transfected at a concentration of 140 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL polyethylenimine (PEI, Polyscience) in PBS was used for the transfection at a ratio of 3:1 (μL:μg ...
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Biochemistry 2021Quote: ... Forty microliters of 2 mg/mL PEI “Max” (Polyscience) was diluted in 1.25 mL of OptiMEM and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.2 µL of 1 mg/mL PEI MAX (Polyscience #24765), and 100 µL of 1x PBS were mixed ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 300 µL of 1 mg/mL polyethylenimine linear 25k (Polyscience, 23966) was diluted into 5 mL OptiMEM in a separate tube ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Immunology 2022Quote: ... in the presence of 198 µl PEI (1mg/ml, PEI MAX, Polyscience). At 48 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were co-transfected using PEI-MAX (1μg/ml; Polyscience, 324765) with VSV-G (Addgene#8454) ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with physiological saline followed by an ice-cold 0.1M phosphate-buffered (pH 7.4) fixative containing 4% PFA and 0.1% glutaraldehyde (Polyscience Europe GmbH, Eppelheim, Germany) for 15min ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of HEK293T cells was carried out using 1 mg/ml Polyethyleneimine MAX (Polyscience). Mycoplasma contamination was monitored by DAPI staining.
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto assay plates and transfected using polyethyleneimine (PEI, Polyscience, 1 mg/mL). Details on the conditions for each experiment can be found in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
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bioRxiv - Cell Biology 2020Quote: ... infected cells were en bloc stained with uranyl acetate and lead aspartate then samples were dehydrated in acetone solutions of increasing concentrations (30-100%) and embedded in PolyBed 812 resin (Polyscience Inc., Warrington, PA, USA) using flat-embedding molds (EMS ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then transfected with either Reelin-HA or control plasmid using 1.0 mg/ml polyethyleneimine MAX (Polyscience). After 2 days ...
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bioRxiv - Microbiology 2023Quote: ... DNA (1 µg/ml final transfection volume) was added followed by addition of linear polyethylenimine (PEI25k, Polyscience, Inc.) (1mg/ml ...
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bioRxiv - Immunology 2019Quote: ... for IgG was mixed with 25 mL Opti-MEM containing 2,250 µg polyethylene imine MAX (MW 40,000; Polyscience - 24765-1). After incubation for 20 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a total of 800 ng of plasmids encoding both Target-AID and gRNA were combined with 2.5 µL of 1 mg/mL PEI MAX (Polyscience #24765) and 200 µL of 1x PBS and applied to each well after 5–10 min incubation at room temperature.
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bioRxiv - Genetics 2023Quote: ... 15 μg DNA was incubated in 600 μL Opti-MEM and 60 μL of 1 mg/mL linear PEI (Polyscience, 23966-2) for 15 minutes at room temperature before adding to cells dropwise ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...