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Citations for PolyScience :
1 - 42 of 42 citations for 6H Pyrazolo 3 4 g benzothiazole 2 methyl 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... ∼2 × 107 3-μm latex NH2-beads (Polyscience) were activated with 8% glutaraldehyde for 4 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: 293T cells were transfected with overexpression constructs along with packaging vectors VSV-G and Delta8.9 using PEI reagent (PEI MAX, Polyscience #24765-2). Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μl Concavalin-A slurry (Polyscience, 86057-3) per sample were activated by washing the bead slurry three times in 1.5 mL Binding Buffer (HEPES pH 7.5 20 mM ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.G (100 ng/well) plasmids using PEI MAX (Polyscience, 24765-100). Cells were incubated at 37C ...
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bioRxiv - Genomics 2023Quote: ... Polyethylenimine (PEI) (Polyscience 23966-2) was added at a 3:1 PEI:DNA ratio and the mixture briefly vortexed and incubated at RT for 10-20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... ~2x 107 3-μm latex NH2-beads (Polyscience, Eppelheim, Germany) were activated with 8% glutaraldehyde for four h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 4% v/v formaldehyde (methanol-free; Polyscience) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... Transfection was conducted with 4 μl of PEI (Polyscience) following the manufacturer’s manual and 1μg of CR plasmids and 1μg of rgRNA plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... root meristems were excised and fixed in 4% formaldehyde (Polyscience) in PBS pH 7.2 for 24 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... contrasted with 2% uranyl acetate (Polyscience, 21447) and lead citrate(Reynold ...
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bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Neuroscience 2022Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were fixed in 2 % paraformaldehyde (Polyscience; Hirschberg an der Bergstrasse ...
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bioRxiv - Developmental Biology 2024Quote: ... elegans were mounted on 2% agarose (w/v in water) dry pads immersed in 2% polystyrene bead (0.1 mm diameter, Polyscience, 00876-15) diluted in M9 buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and pCMV-VSV-G-RSV-Rev (a gift of Dr. Miyoshi, RIKEN, Japan) by using the linear polyethyleneimine “Max” MW 40,000 (Polyscience). After two days ...
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bioRxiv - Biochemistry 2021Quote: ... Forty microliters of 2 mg/mL PEI “Max” (Polyscience) was diluted in 1.25 mL of OptiMEM and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2023Quote: ... and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Center) were co-transfected into Lenti-X 293T cells using polyethyleneimine (Polyscience). Virus-containing media were collected at 48 or 72 h after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
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bioRxiv - Microbiology 2021Quote: Virus stocks were obtained by transfecting of HEK 293T cells with proviral DNA in the presence or absence of pSVCMV-VSV-G using polyethylenimine (PEI; Polyscience, #23966). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Cell Biology 2019Quote: ... and pCMV-VSV-G-RSV-Rev were co-transfected into Lenti-X 293T cells using polyethylenimine (no. 24765-1, Polyscience Inc., Warrington, PA). To prepare the retrovirus ...
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bioRxiv - Cell Biology 2021Quote: ... and pCMV-VSV-G-RSV-Rev were co-transfected into Lenti-X 293T cells using polyethylenimine (no. 24765-1, Polyscience Inc., Warrington, PA). The infected cells were selected with media containing the following antibiotics ...
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bioRxiv - Microbiology 2022Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Developmental Biology 2024Quote: ... mounted in a 3 μL drop of M9 containing approximately fifty 25 μm diameter polystyrene beads (Polyscience Inc.) and sealed under a coverslip using Vaseline ...
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bioRxiv - Immunology 2021Quote: ... pαH-MERS RBD-Fc using 2 mg of polyethyleneimine (Polyscience, Cat# 24765). After 6 days of growth ...
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bioRxiv - Molecular Biology 2023Quote: ... Rev and Gag-pol using PEI reagent (PEI MAX, Polyscience, 24765-2). The supernatant was collected after 48 and 72 h ...
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bioRxiv - Bioengineering 2022Quote: ... The lentiviral vector was cotransfected into HEK293T cells (RCB2202, RIKEN, Japan) with psPAX2 and pCMV-VSV-G-RSV-Rev using Polyethylenimine Max (Polyscience Inc., Warrington, PA, USA). Culture supernatants containing the virus were collected at 48 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected using the PEImax (Cat# 24765-2, MW 40,000, Polyscience, Inc.) at a ratio of 1:3 of DNA ...
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Neuroscience 2019Quote: ... Toluidine blue was performed on sciatic nerve fixed in phosphate buffer 2% glutaraldehyde (Polyscience cat.07710) to assess the number of axons and the myelin thickness ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with physiological saline followed by an ice-cold 0.1M phosphate-buffered (pH 7.4) fixative containing 4% PFA and 0.1% glutaraldehyde (Polyscience Europe GmbH, Eppelheim, Germany) for 15min ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
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bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
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bioRxiv - Biophysics 2023Quote: ... We transfected HeLa cells (ATCC CCL-2) with 10 µg of each px459 v2.0 plasmid together with 40 µl of linear PEI (Polyscience 23966) using standard protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... 15 μg DNA was incubated in 600 μL Opti-MEM and 60 μL of 1 mg/mL linear PEI (Polyscience, 23966-2) for 15 minutes at room temperature before adding to cells dropwise ...