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Citations for Labcyte :
1 - 29 of 29 citations for 7H 1 2 3 Triazolo 4 5 d pyrimidin 7 one 3 6 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... combinations of DMSO or 11 does of sotrastaurin and DMSO or 7 doses of ingenol-3-angelate were dispensed in quadruplicate using an Echo555 (Labcyte). Immunostaining and quantification of the B7-H3 protein expression level were performed as described above in the high-content imaging screening assay method section ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with BRM011, BRM014, or BRM017 (11-point, 3-fold serial dilutions) in triplicate using an Echo550 (Labcyte). Viability was assessed on Day 0 and Day 5 using CTG according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 nL of compounds were dispensed (2 x 2.5 nL fixed dispensing using an Echo550 (Labcyte)) from a concentration stock of 1 mM (100% DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... The plates were briefly centrifuged at 1000 rpm and incubated at 37°C with 5% CO2 for an additional 6 days using MicroClime Environmental lids (Labcyte, San Jose, CA). Plates were placed at room temperature for 30 min to equilibrate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... is transferred into empty wells in columns 3 and 13 of an Echo® Qualified 384-Well Polypropylene Source Microplate (Labcyte P-05525). Compounds are diluted 2-fold by transferring 40 μL of each sample into 40 μL DMSO in the adjacent well (columns 4 and 14 ...
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bioRxiv - Biochemistry 2020Quote: ... was added 7-amino-methylcoumarin (62.5 nL) and inhibitor or DMSO (62.5 nL) using an ECHO 655 acoustic dispenser (LabCyte). To that was added assay buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 nL of 1 mM MLN4924 in DMSO was added to every well using an acoustic liquid handler (Labcyte Echo 500), and the plates were returned to the incubator for an additional 17 hours ...
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bioRxiv - Immunology 2020Quote: ... and compounds in DMSO were dispensed into columns 5-48 via acoustic dispensing (Labcyte Echo 655, San Jose, CA). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
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bioRxiv - Biochemistry 2022Quote: To a 1536-well black plate was added 7-amino-4-methylcoumarin (0.25 mM, 20 nL, 250x) and inhibitor (20 nL, 250x) to columns 5-48 using an ECHO 655 acoustic dispenser (LabCyte). To the control columns 1-2 was added 7-amino-4-methylcoumarin (0.25 mM ...
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bioRxiv - Biochemistry 2019Quote: ... To limit the concentration error due to the extremely low assay volume (5 µl) peptide dilution series as well as ATP and CK1δ addition were performed by an Echo 550 (Labcyte) ultrasonic liquid handler ...
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bioRxiv - Biochemistry 2022Quote: The DSF assay plate was constructed by dry-spotting 5 nL of SYPRO Orange with an acoustic dispenser (Echo 555, Labcyte) into a 384-well PCR plate ...
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bioRxiv - Cell Biology 2019Quote: ... 150 nl of an siRNA oligo solution at 5 μM concentration were spotted at the bottom of imaging plates using an Echo525 instrument (Labcyte). In these cases ...
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bioRxiv - Microbiology 2020Quote: ... DNA extracts were normalized to 5 ng total input per sample using an Echo 550 acoustic liquid handling robot (Labcyte Inc), and 1/10 scale enzymatic fragmentation ...
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bioRxiv - Systems Biology 2024Quote: ... DNA extracts were normalized to 5 ng total input per sample using an Echo 550 acoustic liquid handling robot (Labcyte Inc), and 1/10 scale enzymatic fragmentation ...
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Immunology 2021Quote: ... Serum or plasma samples were diluted in sample buffer (1% milk in PBS-T) and dispensed using acoustic dispensing technology employing the ECHO 555 (Labcyte). Thereby ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 or 7.5 nl of a 10 mM stock of the compounds dissolved in DMSO were arrayed and dispensed into square flat-bottom black 384-well plates containing 1 µl DMSO/well using an Echo 550 (Labcyte), before being sealed and stored at −80°C.
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bioRxiv - Biochemistry 2021Quote: ... 2.5 or 7.5 nl of a 10 mM stock of the compounds dissolved in DMSO were arrayed and dispensed into square flat-bottom black 384-well plates containing 1 μl DMSO / well using an Echo 550 (Labcyte), before being sealed and stored at −80°C.
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bioRxiv - Cancer Biology 2020Quote: ... compounds were first stamped in triplicate into 384 well plates for a final concentration of 1 μM using an Echo Acoustic Dispenser (Labcyte). Cells and media were then dispensed into plates using a WellMate (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... Compounds were stamped into 384 well plates for a final concentration of 1 μM using an Echo Acoustic Dispenser (Labcyte). Cells were then plated at a density of 2,000 cells/well using a WellMate (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... 10 nl of 1 uM barcoded DRUG-seq RT primers were then dispensed into each well using an Echo 555 Liquid Handler (Labcyte). Plates were sealed (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... at concentration ranges of 1 nM to 100 M using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte, Sunnyvale, CA), with DMSO being back-added to a final concentration of 0.1% ...
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bioRxiv - Synthetic Biology 2019Quote: ... Reactions were distributed by electronic pipette and TetR dilutions were distributed into cell-free reactions in volumes of 1 µL using an Echo 525 acoustic liquid handler (Labcyte Inc.).
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bioRxiv - Synthetic Biology 2022Quote: ... 384-well plates were prepared for screening by dispensing 1 mM IPTG and 0.5 mM small molecules using an acoustic liquid handler (Labcyte Echo 550). This scheme will generate four data points per variant ...
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bioRxiv - Immunology 2020Quote: ... and serum was pre-diluted 1:20 in sample buffer and transferred to antigen-coated 1536-well assay plates using acoustic dispensing technology (Labcyte ECHO 555) with serial dilutions ranging from 1:5–1:640 (mucosal samples ...
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bioRxiv - Cancer Biology 2022Quote: ... were stamped in triplicate into 384-well plates at a final concentration of 1 μM using an Echo Acoustic Dispenser (Labcyte, Indianapolis, IN, USA). Cells and media were subsequently dispensed into plates using a WellMate (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: Dose-response experiments were performed as previously described.18;33 Compounds (Supplementary Table 1) were pre-printed in 384-well cell culture microplates using the Echo 550 liquid handler (Labcyte Inc., San Jose, CA, USA). Each compound was tested at five different concentrations in ten-fold increments ranging from 1 nM - 10000 nM (0.1 nM - 1000 nM for copanlisib) ...