Labshake search
Citations for Cell Biolabs :
1 - 35 of 35 citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... was mixed with collagen solution in a 1:4 ratio of cell suspension and collagen mixture provided in the cell contraction assay (Cell Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... incubated overnight with anti-His antibody (Cell Biolabs) and finally with horseradish peroxidase-conjugated goat anti-mouse IgG (Jackson Immuno-research Laboratory ...
-
bioRxiv - Developmental Biology 2022Quote: Collagen analysis was performed using the CytoSelect Cell Contraction Assay Kit (Cell Biolabs, Inc). Mitral valves were dissected from control and mutant hearts at E18.5 and were digested with 1mg/ml collagenase and dispase (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2019Quote: ... deGFP concentration was made using recombinant eGFP (Cell Biolabs Inc.).35 All reactions were performed in at least triplicate.
-
bioRxiv - Synthetic Biology 2021Quote: Recombinant GFP (MW, 29 kDa) was acquired from Cell BioLabs and prepared in 1x PBS with a concentration of 1 mg/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... We used HNE-His ELISA Kit (Cell BioLabs, Inc., San Diego, CA, USA) as described in Kandimalla et al 2016 ...
-
bioRxiv - Neuroscience 2023Quote: ... FIB-AIBP-His was transduced by the AAV-DJ/8 Helper-free system (Cell BioLabs) which is mimicking serotypes AAV8 and AAV9 with the inverted terminal repeats (ITRs ...
-
bioRxiv - Cell Biology 2022Quote: ... Collagen gel was prepared by mixing cell suspension and collagen solution and seeded into 45-well plate with Cytoselect Cell contraction Assay Kit (CELL BIOLABS) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... The FLAG-tagged mouse Pdx1 coding sequence was amplified by PCR and subcloned into a pMXs-Neo Retroviral vector (RTV-011, Cell Biolabs, Inc.). pGL3-basic-MafA plasmid (−10427/+22 from transcription start site ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Cell contractile capability was compared in primary VSMCs and HBMVPs treated with WIT003 or HET0016 following the protocol provided by a collagen gel-based cell contraction assay kit (CBA-201, Cell Biolabs, San Diego, CA). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: Cell contractile capabilities were compared using primary cerebral VSMCs (passages 2 – 4) with a collagen gel-based cell contraction assay kit (CBA-201, Cell Biolabs, San Diego, CA) following our optimized protocol 9,40,48,53,57,58 ...
-
bioRxiv - Cancer Biology 2024Quote: Cell migration ability was determined by CytoSelect™ 24-Well Cell Haptotaxis Assay (8 µm, Collagen I-Coated, Colorimetric Format; Cell Biolabs Inc, San Diego, CA, USA). Cells suspension were added to the upper chamber (3 × 104 cells in 300 µl blank medium) ...
-
bioRxiv - Biophysics 2021Quote: ... and the HEK 293AD human embryonic kidney cell line from Cell Biolabs, Inc (AD-100) ...
-
bioRxiv - Neuroscience 2020Quote: ... Type 2 serotypes of rAAV vectors were prepared using the AAV Helper Free Packaging System (Cell Biolabs, San Diego, CA, USA). The serotypes were produced in HEK 293 cells using a helper virus-free system and were purified using two CsCl2 density gradients and titrated by quantitative polymerase chain reaction ...
-
bioRxiv - Molecular Biology 2021Quote: ... and co-transfected with pAAV-DJ vector and pAAV-Helper vector into HEK 293T cells to generate recombinant adeno-associated virus expressing Mir20b or anti-Mir20b according to the manufacturer’s protocol (Cell Biolabs, San Diego, CA). The AAVs were purified with AAVpro® Purification Kit (Takara Bio ...
-
bioRxiv - Cancer Biology 2020Quote: ... the human MIR10B gene was cloned into the pMX-IRES-Blasticidin vector (Cell Biolabs Inc., RTV-016), downstream of the constitutive Pol-III U6 promoter ...
-
bioRxiv - Physiology 2023Quote: ... The AAV-HSA-GFP plasmid was developed by inserting a human HSA promoter and GFP (ZsGreen1) into the promoterless AAV vector (Cell BioLabs, Cat. No. VPK-411-DJ) using In-Fusion Cloning (Takara Bio ...
-
bioRxiv - Neuroscience 2019Quote: ... trans plasmids pAAV2/1 (Cell Biolabs Inc., Cat# VPK-421), pAAV2/2 (Cell Biolabs Inc. ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-GAPDH (1:1,000; Cat#: AKR-001, Cell Biolabs Inc.), rabbit-anti Influenza A H1N1 HA (1:1,000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Viruses were produced with Serotype 1 or DJ (Cell Biolabs, Inc.) and purified by CsCl gradients 67 ...
-
bioRxiv - Immunology 2023Quote: ... 800 µl of 1 x reagent B (Cell Biolabs; Cat; MET-5011) was added to the cells/ tissues and further incubated on ice for 10 mins with occasional vortexing ...
-
bioRxiv - Cancer Biology 2022Quote: The Plat-A retroviral packaging cell line (1 ξ 106 cells, Cell Biolabs) was seeded on collagen I-coated six-well plates (4810-010 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 μl per 100 μl culture medium of WST-1 reagent (Cell Biolabs, CBA-253) were added to each well ...
-
bioRxiv - Immunology 2021Quote: ... Adhesion of THP-1 cells was measured by Cytoselect Leukocyte-Endothelium Adhesion assay kit (Cell Biolabs, Inc) following suppliers protocol.
-
bioRxiv - Physiology 2022Quote: ... and subsequently incubated with primary antibody against DNP (Cell Biolab; 1:1000 dilution 5% FSG TBS-T) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... EV effects on neuronal membrane integrity were assessed using the ethidium homodimer (EthD-1) assay (Cell Biolabs, San Diego, CA), which is based on the detection of the membrane impermeable EthD-1 dye which fluoresces when bound to DNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then blocked for 1 hour at room temperature in blocking buffer containing 5% PhosphoBLOCKER blocking reagent (Cell Biolabs) in TBST ...
-
bioRxiv - Neuroscience 2024Quote: ... 2017) (Suppl. Table 1) targeting the open reading frame into AAV U6 GFP (Cell Biolabs Inc., San Diego, CA, USA) using BamH1 (New England Biolabs ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 ug of targeting vector was co transfected with 40 ug pDP8 and 200 ul PEI (1 mg/ml) in a P15 of 293AAV cells (AAV-100, Cell biolabs), at 40% confluence ...
-
bioRxiv - Molecular Biology 2023Quote: The pri-miR-132 and pri-miR-124 expressing constructs were made by amplifying the pri-miRNA fragments (Supplementary table 1) from miRNASelectTM pEGP-mmu-mirna expression vectors (Cell Biolabs) and subsequently cloning them into the BamHI-BsrGI sites of pRRLSIN.cPPT.SYN.WPRE.
-
bioRxiv - Physiology 2022Quote: ... muscle samples were bead homogenized by a Qiagen Tissuelyser in reducing sample buffer (Tris-HCl, pH 6.8; 2% SDS; 10% glycerol; 1% β-mercaptoethanol) containing protease inhibitors (Cell Biolabs, AKR-190). Samples were centrifuged at 10,000 rpm at 4 °C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... ref MS E-5100) and 8-hydroxy-2’-deoxyguanosine (“8-OHdG,” in ng.ml− 1; OxiSelectTM Oxidative DNA Damage Elisa kit, Cell Biolabs Inc., ref STA-320) were measured in duplicates from urine samples as indicators of the organisms’ response to environmental stress (Miller et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... shown to be effective against Ncan mRNA (Okuda et al., 2014) (Suppl. Table 1) targeting the open reading frame into AAV U6 GFP (Cell Biolabs Inc., San Diego, CA, USA) using BamH1 (New England Biolabs ...
-
bioRxiv - Neuroscience 2019Quote: ... were generated by the insertion of three different siRNA sequences (obtained from Dharmacon, Horizon Discovery, supplementary table 1) into AAV U6 GFP (CELL BIOLABS INC., San Diego, CA 92126, USA) using BamH1 and EcoR1 restriction sites ...